Skip to main content

Protoplast Culture of Hardwoods

  • Chapter
Cell and Tissue Culture in Forestry

Part of the book series: Forestry Sciences ((FOSC,volume 24-26))

Abstract

Hardwood trees, like most woody plants, present difficult challenges to the plant breeder. Not only are the plants unwieldy and thus difficult to manipulate in large numbers, but the life cycle with the attendant juvenile period of years to decades strongly limits the rapidity of improvement. In addition, many hardwood tree species are genetically diverse (heterozygous) and thus complex breeding strategies must be employed to stabilize a genetic character in a seed-propagated population. These characteristics make breeding hardwood trees both time-consuming and expensive. Since most of the hardwood species are not the focus of large forestry programs, such genetic improvement programs are usually not undertaken on an extensive basis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. AHUJA MR 1983 Developmental potential of mega and normal protoplasts in Populus. In I Potrykus, CT Harms, A Hinnen, R Hutter, PJ King, RD Shillito, eds, 6th Int Protoplast Symp, Basel, Experientia Suppl 45:28–29

    Google Scholar 

  2. AHUJA MR 1983 Isolation and culture of mega and normal protoplasts in Aspen. Silvae Genet 32:5–6

    Google Scholar 

  3. AHUJA MR 1984 Protoplast research in woody plants. Silvae Genet 33:32–37

    Google Scholar 

  4. AHUJA MR 1984 Isolation and culture of mesophyll protoplasts from mature beech trees. Silvae Genet 33:37–39

    Google Scholar 

  5. BINDING H, J Jorgensen, G KRUMBIEGEL-Schroeren, J Finger, G Mordhorst, G SUCHWIAT 1983 Culture of apical protoplasts from shoot cultures in the orders Fabales, Rosales, and Caryophyllales. In I Potrykus, CT Harms, A Hinnen, R Hutter, PJ King, RD Shillito, eds, 6th Int Protoplast Symp, Basel, Experientia Suppl 45:34–35

    Google Scholar 

  6. BRIGHT SWJ, DH NORTHCOTE 1974 Protoplast regeneration from normal and bromodeoxuridine-resistant sycamore cells. J Cell Sci 16:445–463

    CAS  Google Scholar 

  7. BURGER DW, WP HACKETT 1981 Protoplast culture of several citrus tissues. Hortscience 16:417

    Google Scholar 

  8. BURGER DW, WP HACKETT 1982 The isolation, culture, and division of protoplasts from citrus cotyledons. Physiol Plant 56:324–328

    Article  Google Scholar 

  9. CARLBERG I, K Glimelius, T ERIKSSON 1983 Improved culture ability of potato protoplasts by use of activated charcoal. Plant Cell Rep 2:223–225

    Article  Google Scholar 

  10. DORION N, B Godin, C BIGOT 1983 Isolation and culture of leaf protoplasts from Ulmus sp. In I Potrykus, CT Harms, A Hinnen, R Hutter, PJ King, RD Shillito, eds, 6th Int Protoplast Symp, Basel, Experientia Suppl 45:8–9

    Google Scholar 

  11. DOUGLAS G 1982 Protoplast isolation from totipotent cell-cultures of Populus hybrid TT32. In A Fujiwara, ed, Plant Tissue Culture 1982, Proc 5th Int Cong Plant Tissue Cell Culture, Tokyo, pp 605–606

    Google Scholar 

  12. HORSCH RB, JE Fry, NL Hoffmann, D Eichholtz, SG Rogers, RT FRALEY 1985 A simple and general method for transferring genes into plants. Science 277:1229–1231

    Google Scholar 

  13. HUHTINEN O, J Honkanen, LK SIMOLA 1982/83 Ornithine and putrescine-supported divisions and cell colony formation in leaf protoplasts of alders (Alnus glutinosa and A. incana). Plant Sci Lett 28:3–9

    CAS  Google Scholar 

  14. HURWITZ CD, GN AGRIOS 1984 Isolation and culture of protoplasts from apple callus and cell suspension cultures. J Am Soc Hortic Sci 109:348–350

    Google Scholar 

  15. KARUNARATNE SM, KJ SCOTT 1981 Mitotic activity in protoplasts isolated from Sorghum bicolor leaves. Plant Sci Lett 23:11–16

    Article  Google Scholar 

  16. KOBAYASHI S, H Uchimiy, I IKEDA 1983 Plant regeneration from ‘Trovita’ orange protoplasts. Jpn J Breed 33:119–122

    Google Scholar 

  17. KOHLENBACH HW, W WERNICKE 1978 Investigations on the inhibitory effect of agar and the function of active carbon in anther culture. Z Pflanzenphysiol 86:463–472

    CAS  Google Scholar 

  18. KOUIDER M, R Hauptmann, JM Widholm, RM Skirvin, SS KORBAN 1984 Callus formation from Malus x domestica cv. ‘Jonathan’ protoplasts. Plant Cell Rep 3:142–145

    Article  Google Scholar 

  19. KRISHNAMURTHY KV, RR Hendre, DA Godbole, VM Kulkarni, AF Mascarenhas, V JAGANNATHAN 1979 Isolation and regeneration of rose bud callus protoplasts (Rosa sp. cv. Soraya). Plant Sci Lett 15:135–137

    Article  Google Scholar 

  20. MORENO V, L Zubeldia, LA ROIG 1984 A method for obtaining callus cultures from mesophyll protoplasts of melon (Cucumis melo L.). Plant Sci Lett 34:195–201

    Article  Google Scholar 

  21. OHYAMA K, S OKA 1975 Isolation of protoplasts from mesophyll cells of Morus alba. Proc Crop Sci Soc Jpn 44:121–122

    Article  Google Scholar 

  22. OKAMURA M, T Hayashi, S MIYAZAKI 1984 Inhibiting effect of ammonium ion in protoplast culture of some Asteraceae plants. Plant Cell Physiol 25:281–286

    CAS  Google Scholar 

  23. OROZCO FJ, O SCHIEDER 1983 Isolation and culture of coffee leaf protoplasts. In I Potrykus, CT Harms, A Hinnen, R Hutter, PJ King, RD Shillito, eds, 6th Int Protoplast Symp, Basel, Experientia Suppl 45:52–53

    Google Scholar 

  24. OZEKI Y, A KOMAMINE 1981 Induction of anthocyanin synthesis in relation to embryogenesis in a carrot suspension culture: Correlation of metabolic differentiation with morphological differentiation. Physiol Plant 53:570–577

    Article  CAS  Google Scholar 

  25. OZIAS-AKINS P, PS Rao, O SCHIEDER 1984 Plant regeneration from embryonic suspension-derived protoplasts of sandalwood (Santalum album). In RH Henke, KW Hughes, MJ Constantin, A Hollaender, eds, Tissue Culture in Forestry and Agriculture, Proceedings of the Third Tennessee Symposium on Plant Cell and Tissue Culture, Plenum Press, New York, pp 338–339

    Google Scholar 

  26. PATEL KR, NS Shekhawat, GP Berlyn, TA THORPE 1984 Isolation and culture of protoplasts from cotyledons of Pinus coulteri D.Don. Plant Cell Tissue Organ Culture 3:85–90

    Article  Google Scholar 

  27. PEARCE RS, EC COCKING 1973 Behaviour in culture of isolated protoplasts from “Paul’s Scarlett” rose suspension culture cells. Protoplasma 77:165–180

    Article  Google Scholar 

  28. RADOJEVIC L, A KOVOOR 1978 Characterization and estimation of newly synthesized DNA in higher plant protoplasts during the initial period of culture. J Exp Bot 29:963–968

    Article  CAS  Google Scholar 

  29. RAO PS, P OZIAS-AKINS 1985 Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.) Protoplasma 124:80–86

    Article  Google Scholar 

  30. REDENBAUGH K, DF Karnosky, RD WESTFALL 1981 Protoplast isolation and fusion in three Ulmus species. Can J Bot 59:1436–1443

    Article  Google Scholar 

  31. REDENBAUGH MK, RD Westfall, DF KARNOSKY 1980 Protoplast isolation from Ulmus americana pollen mother cells, tetrads and microspores. Can J For Res 10:284–289

    Article  Google Scholar 

  32. ROBENEK H, E PEVELING 1977 Ultrastructure of the cell wall regeneration of isolated protoplasts of Skimmia japonica Thunb. Planta 136:135–145

    Article  Google Scholar 

  33. RUSSELL JA, BH Mccown, in preparation

    Google Scholar 

  34. RUSSELL JA, EL Zeldin, BH MCCOWN 1985 Leaf grinding improves release of mesophyll protoplasts. Hortscience 20:571

    Google Scholar 

  35. SAITO A 1976 Isolation of protoplasts from mesophyll cells of Paulownia fortunei Hemsl. and Populus euramericana cv. I-45/51. J Jpn For Sci 58:301–305

    Google Scholar 

  36. SAITO A 1980 Isolation of protoplasts from mesophyll cells of Paulownia and Populus. Bulletin of the Forestry and Forest Products Research Institute (Japan) 309:1–6

    Google Scholar 

  37. SAITO A 1980 Fusion of protoplasts isolated from somatic cells of tree species. Bulletin of the Forestry and Forest Products Research Institute (Japan) 309: 7–12

    Google Scholar 

  38. SCHIEDER O 1980 Somatic hybrids between a herbaceous and two tree Datura species. Z Pflanzenphysiol 98:119–127

    Google Scholar 

  39. SELLMER JC, JA Russell, EL Zeldin, BH MCCOWN 1985 Utilization of cytokinin response curves in tissue evaluation of Populus for biotechnology research. Hortscience 20:593

    Google Scholar 

  40. SHEPARD JF 1980 Mutant selection and plant regeneration from potato mesophyll protoplasts. In B Gengenbach, RL Phillips, CE Green, eds, Genetic Improvement of Crops: Emergent Techniques, Univ Minnesota press, Minneapolis, pp 185–219

    Google Scholar 

  41. SHEPARD JF, RE TOTTEN 1977 Mesophyll cell protoplasts of potato. Isolation, proliferation, and plant regeneration. Plant Physiol 60:313–316

    Article  PubMed  CAS  Google Scholar 

  42. SITA GL, BS RANI 1983 Preliminary studies on isolation and culture of protoplasts from sandalwood (Santalum album). In I Potrykus, CT Harms, A Hinnen, R Hutter, PJ King, RD Shillito, eds, 6th Int Protoplast Symp, Basel, Experientia Suppl 45:4–5

    Google Scholar 

  43. SKELSEY AF 1984 Biotechnology in agriculture — New tools for the oldest science. Joint Council on Food and Agricultural Science, USDA, 36 pp

    Google Scholar 

  44. SMITH MAL 1984 Application of Microculture and Protoplast Culture Techniques to Physiological Analysis of Woody Crops, PhD Thesis, Univ Wisconsin, Madison

    Google Scholar 

  45. SMITH MAL, BH MCCOWN 1982/83 A comparison of source tissue for protoplast isolation from three woody plant species. Plant Sci Lett 28:149–156

    Google Scholar 

  46. SMITH MAL, JP Palta, BH MCCOWN 1984 The measurement of isotonicity and maintenance of osmotic balance in plant protoplast manipulations. Plant Sci Lett 33:249–258

    Article  CAS  Google Scholar 

  47. SONDAHL MR, MS Chapman, WR SHARP 1980 Protoplast liberation, cell wall reconstitution, and callus proliferation in Coffea arabica L. callus tissues. Turrialba 30:161–165

    Google Scholar 

  48. STICKLEN MB, RD Lineberger, SC DOMIR 1985 Isolation and culture of Ulmus x ‘Homestead’ protoplasts. Hortscience 20:571

    Google Scholar 

  49. STICKLEN MB, RD Lineburger, SC DOMIR 1985 Isolation and culture of protoplasts of Ulmus x ‘Homestead’. Plant Sci 41:117–120

    Article  CAS  Google Scholar 

  50. STRAUSS A, I POTRYKUS 1980 Callus formation from protoplasts of cell suspension cultures of Rosa ‘Paul’s Scarlett’. Physiol Plant 48:15–20

    Article  Google Scholar 

  51. SUGAWARA Y, K Mori, H Matsushima, M TAKEUCHI 1983 Enhancement of cell division in Marchantia protoplast culture by activated charcoal. Z Pflanzenphysiol 109:275–278

    CAS  Google Scholar 

  52. TAKEDA J, M SENDA 1984 Relationship of the electrophysio-logical property of tobacco cultured cells to their regeneration ability. Plant and Cell Physiology 25:619–624

    CAS  Google Scholar 

  53. TREMBLAY FM, JB Power, M LALONDE 1985 Callus regeneration from Alnus incana protoplasts isolated from cell suspensions. Plant Sci 41:211–216

    Article  Google Scholar 

  54. UPADHYA M 1975 Isolation and culture of mesophyll proroplasts of potato (Solanum tuberosum L.). Potato Res 18:438–445

    Article  Google Scholar 

  55. VARDI A, P SPEIGEL-ROY, E GALUN 1975 Citrus cell culture: Isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. Plant Sci Lett 4:231–236

    Article  Google Scholar 

  56. VARDI A, P SPEIGEL-ROY, E GALUN 1982 Plant regeneration from Citrus protoplasts: variability in methodological requirements among cultivars and species. Theor Appl Genet 62:171–176

    Article  Google Scholar 

  57. VENKETESWARAN S, V GANDHI 1980 Protoplast isolation and culture of tree genera for biomass production. Eur J Cell Biol 22:501

    Google Scholar 

  58. VERMA DC, SR WANN 1983 Isolation of high yields of viable protoplasts from quaking aspen seedlings and cultured loblolly pine cell suspensions, In I Potrykus, CT Harms, A Hinnen, R Hutter, PJ King, RD Shillito, eds, 6th Int Protoplast Symp, Basel, Experientia Suppl 45:10–11

    Google Scholar 

  59. WU SC, AH KUNIYUK 1985 Isolation and culture of almond protoplasts. Plant Sci 41:55–60

    Article  CAS  Google Scholar 

  60. ZAPATA FJ, KC Sink, EC COCKING 1981 Callus formation from leaf mesophyll protoplasts of three Lycopersicon species: L. esculentum. cv. Walter, L. pimpinillifolium and L. hirsutum. f. glabratum. Plant Sci Lett 23: 41–46

    Article  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Martinus Nijhoff Publishers, Dordrecht

About this chapter

Cite this chapter

McCown, B.H., Russell, J.A. (1987). Protoplast Culture of Hardwoods. In: Bonga, J.M., Durzan, D.J. (eds) Cell and Tissue Culture in Forestry. Forestry Sciences, vol 24-26. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4484-8_3

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-4484-8_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8497-0

  • Online ISBN: 978-94-009-4484-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics