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Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.)

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Summary

Protoplasts were isolated from embryogenic cell suspension cultures derived from proliferating shoot segments of a 20-year-old sandalwood tree (Santalum album Linn.). Under appropriate conditions, isolated protoplasts divided in liquid culture medium and produced embryogenic cell aggregates and globular embryos. Plating of cell aggregates on a fresh medium facilitated the differentiation of somatic emryos which further developed into plantlets.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3 :

Gibberellic acid

IAA:

indoleacetic acid

IBA:

indolebutrytic acid

MES:

2-(N-morpholino)ethane sulfonic acid

MS:

Murashige and Skoog's medium as modified in the text

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Authors and Affiliations

  1. Independent Research Group Schieder, Max-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut), Köln

    P. S. Rao & Peggy Ozias-Akins

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  1. P. S. Rao
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  2. Peggy Ozias-Akins
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Rao, P.S., Ozias-Akins, P. Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.). Protoplasma 124, 80–86 (1985). https://doi.org/10.1007/BF01279726

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  • DOI: https://doi.org/10.1007/BF01279726

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