Abstract
The cyanobiont of Cycas circinalis (identified as Nostoc sp.) was isolated and its heterotrophic metabolism was studied in free culture under nitrogen-fixing conditions. Morphology, growth rate, nitrogenase activity, biochemical composition, efficiency of assimilation of organic carbon and molecular nitrogen were determined under different conditions of energy and carbon supply. The study has revealed the high potential of the heterotrophic metabolism in this symbiotic cyanobacterium. Although low rates of metabolic activities were attained under heterotrophic conditions, the efficiencies of organic carbon utilization (0.48 g cell-carbon per g glucose-carbon in chemoheterotrophy, from 0.65 to 0.74 under photoheterotrophy) and of N2 assimilation (35.0 mg N2 fixed per g glucose used in chemoheterotrophy, from 58.3 to 61.9 under photoheterotrophy) displayed by this organism were among the highest ever found in diazotrophically grown micro-organisms. The isolate from C. circinalis was able to grow indefinitely in the dark under nitrogen-fixing conditions, maintaining a well balanced biosynthetic activity and the capacity to resume photosyntheticmetabolism quickly. The significance of the heterotrophic potential of this symbiotic Nostoc is discussed.
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References
Alexander M 1977 Introduction to Soil Microbiology. John Wiley and Sons, New York, 289 p.
Bottomley P J and Van Baalen C 1978a Characteristics of heterotrophic growth in the blue-green alga Nostoc sp. strainMac. J. Gen. Microbiol. 107, 309–318.
Bottomley P J and Van Baalen C 1978b Dark hexose metabolism by photoautotrophically and heterotrophically grown cells of the blue-green alga (cyanobacterium) Nostoc sp. strain Mac. J. Bacteriol. 135, 888–894.
Dalton H and Postgate J R 1969 Growth and physiology of Azotobacter chroococcum in continuous culture. J. Gen.Microbiol. 56, 307–319.
Dubois M, Gilles K A, Hamilton J K, Rebers P A and Smith F 1956 Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356.
Grilli Caiola M 1980 On the phycobionts of the cycad coralloid roots. New Phytol. 85, 537–544.
Haury J F and Spiller H 1981 Fructose uptake and influence on growth of and nitrogen fixation by Anabaena variabilis. J. Bacteriol. 147, 227–235.
Herbert D, Phipps P J and Strange RE 1971 Chemical analysis of microbial cells. In Methods in Microbiology. Eds. J R Norris and D W Ribbons. Vol. 5B, pp 209–344. AcademicPress, London.
Hoare D S, Ingram L O, Thurston E L and Walkup R 1971 Dark heterotrophic growth of an endophytic blue-green alga. Arch. Mikrobiol. 78, 310–321.
Ingram L O, Calder J A, Van Baalen C, Plucker F E and Parker P L 1973 Role of reduced exogenous organic compounds in the physiology of the blue-green bacteria (algae): photoheterotrophic growth of a “heterotrophic” blue-green bacterium. J. Bacteriol. 114, 695–700.
Jensen B B 1983 Energy requirement for diazotrophic growth of the cyanobacterium Anabaena variabilis determined from growth yield in the dark. J. Gen. Microbiol. 129, 2633–2640.
Khoja T and Whitton B A 1971 Heterotrophic growth of blue-green algae. Arch. Mikrobiol. 79, 280–282.
Koening F 1985 Shade adaptation in the blue-green alga Anacystis nidulans. 5th Int. Symp. Photosynthetic Prokaryotes, Grindelwald 22–28 September.
Lazaroff N 1973 Photomorphogenesis and nostocacean development. In The Biology of Blue-green Algae. Eds. N G Carr and B A Whitton. pp. 279–319. Blackwell Scientific Publications, Oxford.
Newton J W and Herman A I 1979 Isolation of cyanobacteria from the aquatic fern Azolla. Arch. Microbiol. 120, 161–165.
Ogawa T and Aiba S 1981 Bioenergetic analysis of mixotrophic growth in Chlorella vulgaris and Scenedesmus acutus. Biotech. Bioeng. 23, 1121–1132.
Payne W J 1970 Energy yields and growth of heterotrophs. Annu. Rev. Microbiol. 24, 17–52.
Pelroy R A, Kirk M R and Bassham J A 1976 Photosystem II regulation of macromolecule synthesis in the blue-green algaAphanocapsa 6714. J. Bacteriol. 128, 623–632.
Rippka R, Deruelles J, Waterbury J B, Herdman M and Stanier R J 1979 Generic assignments, strain histories and propertiesof pure cultures of cyanobacteria. J. Gen. Microbiol. 111, 1–61.
Smith A J 1982 Modes of cyanobacterial carbon metabolism. In The Biology of Cyanobacteria. Eds. N G Carr and B A Whitton. pp 47–85. Blackwell Scientific Publications, Oxford.
Smith A J 1983 Modes of cyanobacterial carbon metabolism. Ann. Microbiol. (Inst. Pasteur) 134 B, 93–113.
Stacey G, Van Baalen C and Tabita F R 1977 Isolation and characterization of a marine Anabaena sp. capable of rapid growth on molecular nitrogen. Arch. Microbiol. 114, 197–201.
Stewart W D P, Rowell P and Rai A N 1983 Cyanobacteria-eukaryotic plant symbioses. Ann. Microbiol. (Inst. Pasteur) 134 B, 205–228.
Tel-Or E, Sandovsky T, Kobiler D, Arad H and Weinberg R 1983 The unique properties of the symbiotic Anabaena in the water fern Azolla. In Photosynthetic Prokaryotes: Cell Differentiation and Function. Eds. G C Papageorgiou and L Packer, pp 303–314. Elsevier Scientific Publishing Company, New York.
Turner G L and Gibson A H 1980 Measurement of nitrogen fixation by indirect means. In Methods for Evaluating Biological Nitrogen Fixation. Ed. F J Bergersen. pp. 111–138. John Wiley and Sons, New York.
Vaara T, Sivonen K and Kurkela S 1984 Mixotrophic growth of Nostoc 268. In Advances in Photosynthesis Research. Ed. C Sybesma. pp. 809–812. Nijhoff and Junk Publishers, Dordrecht.
Vincenzini M, De Philippis R, Ena A and Florenzano G 1986 Ammonia photoproduction by Cyanospira rippkae cells entrapped in dialysis tube. Experientia 42, 1040–1043.
Wolk C P and Shaffer P W 1976 Heterotrophic micro-and macrocultures of a nitrogen-fixing cyanobacterium. Arch. Microbiol. 110, 145–147.
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Tredici, M.R., Margheri, M.C., Giovannetti, L., De Philippis, R., Vincenzini, M. (1989). Heterotrophic metabolism and diazotrophic growth of Nostoc sp. from Cycas circinalis . In: Skinner, F.A., Boddey, R.M., Fendrik, I. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 35. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0889-5_7
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DOI: https://doi.org/10.1007/978-94-009-0889-5_7
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