Abstract
Anabaena variabilis can be cloned in the dark from fragments with one and few cells, with an efficiency of about 40%, on the nitrogen-free medium of Allen and Arnon solidified with 0.5% agarose and supplemented with 5 mM fructose. The organism can be grown exponentially (τ2≅36 h) in fermentor cultures in the dark, fixing N2, to a density of greater than 10 g dry weight/l.
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Peter Wolk, C., Shaffer, P.W. Heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium. Arch. Microbiol. 110, 145–147 (1976). https://doi.org/10.1007/BF00690221
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DOI: https://doi.org/10.1007/BF00690221