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Technologies for Detecting Metals in Single Cells

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Metallomics and the Cell

Part of the book series: Metal Ions in Life Sciences ((MILS,volume 12))

Abstract

In order to fully understand the metallomics of an organism, it is essential to know how much metal is present in each cell and, ideally, to know both the spatial and chemical distributions of each metal (i.e., where within the cell is a metal found, and in what chemical form). No single technique provides all of this information. This chapter reviews the various methods that can be used and the strengths and weaknesses of each.

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Abbreviations

EDS:

electron dispersive spectroscopy

EELS:

electron energy loss spectroscopy

EPMA:

electron probe microanalysis

ESI:

electrospray ionization

FRET:

Förster resonance energy transfer

ICP:

inductively coupled plasma

IR:

infrared

MS:

mass spectrometry

MALDI:

matrix-assisted laser desorption/ionization

MRI:

magnetic resonance imaging

PIXE:

proton induced X-ray emission (sometimes particle instead of proton)

SE:

secondary electron

SIMS:

secondary ion mass spectrometry

SXRF:

synchrotron induced X-ray fluorescence

TOF:

time-of-flight

XANES:

X-ray absorption near edge structure

XAS:

X-ray absorption spectroscopy

XFM:

X-ray fluorescence microscopy

XRF:

X-ray fluorescence

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Acknowledgments

  Some of the measurements described in this chapter were supported by the National Institutes of Health, Grants GM-38047 and GM-70545. The XRF imaging work that was described was all measured at one or more national synchrotron laboratories, much of it at laboratories supported by the US Department of Energy Office of Science.

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Authors and Affiliations

  1. Departments of Chemistry and Biophysics, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, USA

    James E. Penner-Hahn

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  1. James E. Penner-Hahn
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Correspondence to James E. Penner-Hahn .

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  1. Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, 50019, Firenze, Italy

    Lucia Banci

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Penner-Hahn, J.E. (2013). Technologies for Detecting Metals in Single Cells. In: Banci, L. (eds) Metallomics and the Cell. Metal Ions in Life Sciences, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5561-1_2

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