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MicroRNAs for Enhancement of CHO Cell Proliferation and Stability: Insights from Neuroblastoma Studies

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MicroRNAs as Tools in Biopharmaceutical Production

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Abstract

The large-scale production of protein demands cell lines that have high proliferative potential and minimal tendencies to undergo apoptosis or senescence. Efforts in biotechnology have emphasized improving the genetic characteristics of CHO cells to enhance protein production. Many of the features of cell lines that are desirable for biotechnology enterprises, however, are the subject of anti-cancer research. Anti-cancer therapy attempts to decrease cell proliferation rates and increase the potential for cell differentiation and apoptosis to occur. Thus, it is possible for biotechnology oriented research to gain considerable insight from therapeutic targets identified in cancer related studies. Mature microRNAs (miRNA) are 19–22 nt RNA sequences that negatively regulate gene expression at a post-transcriptional level which are emerging as potential therapeutic targets for neoplasia. MiRNAs are significantly involved with cell proliferation, apoptosis, differentiation, senescence, cell migration and invasion in the context of both normal developmental processes and in malignant diseases. They can both promote or retard all of these cellular phenotypes, dependent on cellular context. The purpose of this chapter is to review the phenotypic effects of miRNAs in cancer, using the childhood tumor neuroblastoma as a model, with a view towards understanding how manipulation of miRNAs in CHO cells might improve the phenotypic features of the lines for biotechnology purposes.

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Authors and Affiliations

  1. Cancer Genetics, Royal College of Surgeons in Ireland, Dublin, Ireland

    Raymond L. Stallings

  2. National Children’s Research Centre, Our Lady’s Children’s Hospital, Dublin, Ireland

    Raymond L. Stallings

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  1. Raymond L. Stallings
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Correspondence to Raymond L. Stallings .

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  1. , National Institute for Cellular Biotechn, Dublin City University, Dublin, Ireland

    Niall Barron

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Stallings, R. (2012). MicroRNAs for Enhancement of CHO Cell Proliferation and Stability: Insights from Neuroblastoma Studies. In: Barron, N. (eds) MicroRNAs as Tools in Biopharmaceutical Production. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5128-6_6

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