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MicroRNAs: tiny targets for engineering CHO cell phenotypes?

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Abstract

The ability of microRNAs to influence gene expression is now recognized as a fundamental layer of regulation within the cell. MicroRNAs have a major impact on most biological processes and have generated considerable interest as potential biomarkers as well as therapeutic or engineering targets. In this review we provide a brief overview of their biogenesis, genomic organization and mode of action, followed by a description of the methods and approaches to studying their expression. We go on to consider some of the approaches to utilizing them as tools and their potential application in the bioprocessing area, with particular emphasis on Chinese hamster ovary cell engineering.

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Acknowledgement

The authors’ research is supported by funding from Science Foundation Ireland.

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  1. National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland

    Niall Barron, Noelia Sanchez, Paul Kelly & Martin Clynes

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  1. Niall Barron
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  2. Noelia Sanchez
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  3. Paul Kelly
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  4. Martin Clynes
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Correspondence to Niall Barron.

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Barron, N., Sanchez, N., Kelly, P. et al. MicroRNAs: tiny targets for engineering CHO cell phenotypes?. Biotechnol Lett 33, 11–21 (2011). https://doi.org/10.1007/s10529-010-0415-5

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