Abstract
Embryonic stem (ES) cells can self-renew in culture and are pluripotent, giving rise to a variety of differentiated cell types. More complete utilization of this potential, however, requires more efficient differentiation of ES cells into specific lineages, including neural stem (NS) cells, which can generate functioning cells suitable for the functional recovery of damaged tissues, including neurons. The development of methods to effectively differentiate ES cells into highly homogeneous NS cells via NS spheres, and of proteomic methods of protein identification may allow the elucidation of differentially expressed genes in NS cells, thus providing insights into the molecular events associated with the transition from ES cells to NS cells. This in turn will help facilitate clinical applications of these ES cell-derived NS cells to treat neurological diseases.
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Acknowledgements
The authors express their thanks to Professor Hisashi Hirano of Yokohama City University for liquid chromatography-tandem mass spectrometry with education, training, and promotion of proteomics (Nakamura and Hirano 2008).
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Akama, K., Nakayama, T., Otsu, M., Toda, T., Inoue, N. (2012). Neural Stem Cells Differentiated from Embryonic Stem Cells: Proteomic Identification of Expressed Genes. In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 5. Stem Cells and Cancer Stem Cells, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2900-1_25
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DOI: https://doi.org/10.1007/978-94-007-2900-1_25
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