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Molecular Detection of Somaclonal Variation in Date Palm

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Date Palm Biotechnology

Abstract

Somaclonal variation is a general phenomenon in tissue culture-regenerated plants of many species. Several typical abnormal phenotypes have been detected in tissue culture-propagated date palms. Variation from the source phenotypes is undesirable in the commercial propagation of specific cultivars, and efforts have been made to eliminate the formation of these off-types. Some of these phenotypes resemble off-type phenotypes occurring in other crop plants, such as banana and oil palm. Genetic variation, and altered DNA methylation patterns and gene expression are associated with these abnormal phenotypes. However, the specific key factors involved in their generation are still unknown and efficient markers for their early identification do not exist. The newly available draft of the date palm genome sequence and high-throughput sequencing techniques will enable genomic and transcriptomic analyses of off-types, which in turn will provide a better understanding of the phenomenon of somaclonal variation.

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Acknowledgments

Research on somaclonal variation of date palms was supported by Research Grant Award no. IS-3886-06 from BARD, United States-Israel Binational Agricultural Research and Development Fund. This manuscript is contribution no. 114/2010 from the Volcani Research Center, Israel.

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  1. Department of Fruit Tree Sciences, Institute of Plant Sciences, Volcani Research Center, Bet Dagan, 50250, Israel

    Y. Cohen

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  1. , Department of Applied Biology, University of Helsinki, Latokartanonkaari 5, Helsinki, 00014, Finland

    Shri Mohan Jain

  2. , Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, 31982, Saudi Arabia

    Jameel M. Al-Khayri

  3. Middlebrook Ave 3726, Cincinnati, 45208, Ohio, USA

    Dennis V. Johnson

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Cohen, Y. (2011). Molecular Detection of Somaclonal Variation in Date Palm. In: Jain, S., Al-Khayri, J., Johnson, D. (eds) Date Palm Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1318-5_11

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