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Optimisation of Cultural and Nutritional Parameters for the Production of Protease from Newly Isolated Bacterial Strain Bacillus SDR 10

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Prospects in Bioscience: Addressing the Issues

Abstract

The newly isolated bacterial strain SDR-10 showing growth-associated protease production was a gram-positive rod-shaped bacterium producing endospores. The partial gene sequence of the 16S rRNA gene of the strain followed by sequence alignment confirmed the genus as Bacillus. Bacillus SDR-10 showed a maximum protease production in 24 h. The enzyme showed its optimum temperature of activity at 45°C and optimum pH at 10 and stability at this temperature and pH. Production of protease from the strain was enhanced by optimising the cultural and nutritional parameters. Wheat bran resulted in the highest protease production at a concentration of 0.6%. Inorganic nitrogen sources were poor in inducing enzyme production from the culture. Among the carbon sources studied, glucose was the best for protease production at a level of 1.0%. Factors affecting the enzyme production were maximum at growth pH 6.5 and inoculum size of 5%. Optimisation of the various factors resulted in enhancing the production from 56.51 units to 132.48 units (2.35%).

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Acknowledgement

The authors are thankful to the Environmental Technology Division, National Institute for Interdisciplinary Science and Technology, Thiruvan­anthapuram 695019, for the scientific assistance. We are also grateful to the Director, Department of Collegiate Education, Government of Kerala, India, and the Principal, University College, Thiruvananthapuram.

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Authors and Affiliations

  1. Department of Botany and Research Centre, University College, Thiruvananthapuram, 695034, Kerala, India

    Dinta Daniel, B. S. Rakhi, S. Subramaniyan & G. S. Sandhia

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  1. Dinta Daniel
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  2. B. S. Rakhi
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  3. S. Subramaniyan
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  4. G. S. Sandhia
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Correspondence to G. S. Sandhia .

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Editors and Affiliations

  1. , Dept of Biotechnology & Microbiology, Kannur University, Palayad P.O,Thalassery Campus, Kannur, 670661, Kerala, India

    Abdulhameed Sabu

  2. , Dept. of Biotechnology and Microbiology, Kannur University, Palayad P.O, Thalassery Campus, Kannur, 670661, Kerala, India

    Anu Augustine

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Daniel, D., Rakhi, B.S., Subramaniyan, S., Sandhia, G.S. (2012). Optimisation of Cultural and Nutritional Parameters for the Production of Protease from Newly Isolated Bacterial Strain Bacillus SDR 10. In: Sabu, A., Augustine, A. (eds) Prospects in Bioscience: Addressing the Issues. Springer, India. https://doi.org/10.1007/978-81-322-0810-5_8

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