Abstract
The results presented in this chapter are mostly based on our own work with Hyoscyamus muticus carried out during the years 1982–1998 in our laboratories in Finland and Italy.
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References
Abraham TW, Leete E (1995) New intermediate in the biosynthesis of the tropane alkaloids in Datura innoxia. J Am Chem Soc 117: 8100–8105
Altabella T, Angel E, Biondi S, Palaz6n J, Bagni N, Pinol MT (1995) Effect of the rol genes from Agrobacterium rhizogenes on polyamine metabolism in tobacco roots. Physiol Plant 95: 479–485
Altamura MM, Torrigiani P, Falasca G, Rossini P, Bagni N (1993) Morphofunctional gradients in superficial and deep tissues along tobacco stem: polyamine levels, biosynthesis and oxidation, and organogenesis in vitro. J Plant Physiol 142: 543–551
Altman A (1989) Polyamines and plant hormones. In: Bachrach U, Heimer YM (eds) The physiology of polyamines, vol II CRC Press, Boca Raton, pp 121–146
Bagni N, Altamura MM, Biondi S, Mengoli M, Torrigiani P (1993) Polyamines and morphogenesis in normal and transgenic plant cultures. In: Roubelakis-Angelakis KA, Tran Thanh Van K (eds) Morphogenesis in plants. Plenum Press, New York, pp 89–111
Bagni N, Bongiovanni B, Franceschetti M,Tassoni A (1997) Abscisic acid mutants affect polyamine metabolism. Plant Biosyst 131: 181–187
Baldwin IT, Zhang Z-P, Diab N, Ohnmeiss TE, McCloud ES, Lynds GY, Schmelz EA (1997) Quantification, correlations and manipulations of wound-induced changes in jasmonic acid and nicotine in Nicotiana sylvestris. Planta 201: 397–404
Ben-Hayyim G, Damon J-P, Martin-Tanguy J, Tepfer D (1994) Changing root system architecture through inhibition of putrescine and feruloyl putrescine accumulation. FEBS Lett 342: 145–148
Berlin J (1990) Screening and selection for variant cell lines with increased levels of secondary metabolites. In: Charlwood BV, Rhodes MJC (cds) Secondary products from plant tissue cultures. Oxford Science Publication, Clarendon Press, Oxford, pp 119–137
Bhadra R, Vani S, Shanks JV (1993) Production of indole alkaloids by selected hairy root lines of Catharanthus roseus. Biotechnol Bioeng 41: 581–592
Biondi S, Mengoli M, Mott D, Bagni N (1993) Hairy root cultures of Hyoscyamus muticus: effect of polyamine biosynthesis inhibitors. Plant Physiol Biochem 31: 51–58
Biondi S, Formale S, Oksman-Caldentey K-M, Eeva M, Agostani S, Bagni N (2000) Jasmonates induce over-accumulation of methylputrescine and hydroxycinnamoyl amide content in Hyoscyamus muticus L. root cultures. Plant Cell Rep 19: 691–697
Brodelius P, Funk C, Häner A, Villegas M (1989) A procedure for the determination of optimal chitosan concentration for elicitation of cultured plant cells. Phytochemistry 28: 2651–2654
Burtin D, Michael AJ (1997) Over-expression of arginine decarboxylase in transgenic plants. Biochem J 325: 331–337
Chang P-F L, Xu Y, Narasimhan ML, Cheah KT, Paino D’Urzo M, Damsz B, Kononowicz AK, Abad L, Hasegawa PM, Bressan RA (1997) Induction of pathogen resistance and pathogenesis-related genes in tobacco by a heat-stable Trichoderma mycelial extract and plant signal messengers. Physiol Plant 100: 341–352
Chilton M-D, Tepfer DA, Petit A, David C, Casse-Delbart F, Tempé J (1982) Agrobacterium rhi- zogenes inserts T-DNA into the genomes of the host plant root cells. Nature 295: 432–434
Christen P, Aoki T, Shimomura K (1992) Characteristics of growth and tropane alkaloid production in Hyoscyamus albus hairy roots transformed with Agrobacterium rhizogenes A4. Plant Cell Rep 11: 597–600
Cocking EC (1985) Protoplasts from root hairs of crop plants. Bio/Technology 3: 1104–1106
Deno H, Yamagata H, Emoto T, Yoshioka T, Yamada Y, Fujita Y (1987) Scopolamine production by root cultures of Duboisia myoporoides II. Establishment of a hairy root culture by infection with Agrobacterium rhizogenes. J Plant Physiol 131: 315–323
DeScenzo RA, Minocha SC (1993) Modulation of cellular polyamines in tobacco by transfer and expression of mouse ornithine decarboxylase cDNA. Plant Mol Biol 22: 113–127
DiCosmo F, Misawa M (1985) Eliciting secondary metabolism in plant cell cultures. Trends Biotechnol 3: 318–322
Dräger B (1995) Identification and quantification of calystegines, polyhydroxyl nortropane alkaloids. Phytochem Anal 6: 31–37
Dräger B, Funck C, Höhler A, Mrachatz G, Nahstedt A, Portsteffen A, Schaal A, Schmidt R (1994) Calystegines as a new group of tropane alkaloids in Solanaceae. Plant Cell Tissue Organ Cult 38: 235–240
Dunlop DS, Curtis WR (1991) Synergistic response of plant hairy-root cultures to phosphate limitation and fungal elicitation. Biotechnol Prog 7: 434–438
Dunstan WR, Brown H (1899) Occurrence of hyoscyamine in the Hyoscyamus muticus of India. J Chem Soc 5: 72–82
Eeva M, Eibl R, Eibl D, Oksman-Caldentey K-M, Hiltunen R (1997) Comparision of emerged and submersed cultivation methods of transgenic root culture clones of Hyoscyamus muticus. 5th Annu Congr on Medicinal Plant Research, Regensburg, Abstracts, p L01.
Eilert U (1987) Elicitation: Methodology and aspects of application. In: Constabel F, Vasil IK (eds) Cell culture and somatic genetics of plants, vol 4. Academic Press, New York, pp 153–188
Endo T, Yamada Y (1985) Alkaloid production in cultured roots of three species of Duboisia. Phytochemistry 24: 1233–1236
Evans PT, Malmberg RL (1989) Do PAs have roles in plant development? Annu Rev Plant Physiol Plant Mol Biol 40: 235–269
Flores HE, Martin-Tanguy J (1991) Polyamines and plant secondary metabolites. In: Slocum RD, Flores HE (eds) Biochemistry and physiology of polyamines in plants. CRC Press, Boca Raton, pp 57–76
Flores HE, Hoy MW, Pickard JJ (1987) Secondary metabolites from root cultures. Trends Biotechnol 5: 64–68
Ford Y-Y, Ratcliffe RG, Robins RI (1998) In vivo nuclear-magnetic-resonance analysis of polyamine and alkaloid metabolism in transformed root cultures of Datura stramonium L.: evidence for the involvement of putrescine in phytohormone-induced dedifferentiation. Planta 205: 205–213
Galston AW, Kaur-Sawhney R (1990) Polyamines in plant physiology. Plant Physiol 94:406–410 Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158
Geiger PL (1833) Über einige neue giftige organische Alkalien. Justus Liebigs Ann Chem 7: 72–82
Goldmann A, Milat M-L, Ducrot P-H, Lallemand J-Y, Maille M, Lepingel A, Charpin I, Tepfer D (1990) Tropane derivatives from Calystegia sepium. Phytochemistry 29: 2125–2127
Gundlach H, Müller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci USA 89: 2389–2393
Hamill JD, Parr AJ, Rhodes MJC, Robins RI, Walton NJ (1987) New routes to plant secondary products. Bio/Technology 5: 800–804
Hamill JD, Robins RJ, Parr AI, Evans DM, Furze JM, Rhodes MJC (1990) Over-expressing a yeast ornithine decarboxylase gene in transgenic roots of Nicotiana rustica can lead to enhanced nicotine accumulation. Plant Mol Biol 15: 27–38
Hashimoto T, Mitani A, Yamada Y (1990) Diamine oxidase from cultured roots of Hyoscyamus niger. Plant Physiol 93: 216–221
Hashimoto T, Hayashi A, Amano Y, Kohno J, Iwanari H, Usuda S, Yamada Y (1991) Hyoscyamine 613-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root. J Biol Chem 266: 4648–4653
Hashimoto T, Nakajima K, Ongena G, Yamada Y (1992) Two tropinone reductases with distinct stereospecificities from cultured roots of Hyoscyamus niger. Plant Physiol 100: 836–845
Hashimoto T, Matsuda J, Yamada Y (1993a) Two-step epoxidation of hyoscyamine to scopolamine is catalyzed by bifunctional hyoscyamine 63-hydroxylase. FEBS Lett 329: 35–39
Hashimoto T, Yun D-J, Yamada Y (1993b) Production of tropane alkaloids in genetically engineered root cultures. Phytochemistry 32: 713–718
Hibi N, Fujita T, Hatano M, Hashimoto T, Yamada Y (1992) Putrescine N-methyltransferase in cultured roots of Hyoscyamus albus. Plant Physiol 100: 826–835
Hibi N, Higashiguchi S, Hashimoto T, Yamada Y (1994) Gene expression in tobacco low-nicotine mutants. Plant Cell 6: 723–735
Ionkova I, Witte L, Alfermann AW (1994) Spectrum of tropane alkaloids in transformed roots of Datura innoxia and Hyoscyamus x györffyi cultivated in vitro. Planta Med 60: 382–384
Jouhikainen K, Jokelainen T, Hiltunen R, Teeri HT, Oksman-Caldentey K-M (1999) Enhanced scopolamine production in Hyoscyamus hairy root cultures by genetic engineering. Planta 208: 545–551
Jung G, Tepfer D (1987) Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia septum roots grown in vitro. Plant Sci 50: 145–151
Kahlos K, Hiltunen R (1988) Gas chromatographic-mass spectrometric identification of some lanostanes from Inonotus obliquus. Acta Pharm Fenn 97: 45–49
Kanegae T, Kajiya H, Amano Y, Hashimoto T, Yamada Y (1994) Species-dependent expression of the hyoscyamine 643-hydroxylase gene in the pericycle. Plant Physiol 105: 483–490
Knopp E, Strauss A, Wehrli W (1988) Root induction on several Solanaceae species by Agrobacterium rhizogenes and the determination of root tropane alkaloid content. Plant Cell Rep 7: 590–593
Koda Y (1997) Possible involvement of jasmonates in various morphogenic events. Physiol Plant 100: 639–646
Kumar A, Altabella T, Taylor MA, Tiburcio AF (1997) Recent advances in polyamine research. Trends Plant Sci 2: 124–130
Kutchan T (1995) Alkaloid biosynthesis–the basis for metabolic engineering of medicinal plants. Plant Cell 7: 1059–1070
Ladenburg A (1881) Die natürlich vorkommenden mydriatisch wirkenden Alkaloide. Justus Liebigs Ann Chem 206: 274–307
Larkin PJ, Scowcroft WR (1981) Somaclonal variation–a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214
Lee J, Vogt T, Schmidt J, Parthier B, Löbler M (1997) Methyljasmonate-induced accumulation of coumaroyl conjugates in barley leaf segments. Phytochemistry 44: 589–592
Leete E (1958) The biogenesis of nicotine. V. New precursors of the pyrrolidine ring. J Am Chem Soc 80: 2162–2164
Leete E (1979) Biosynthesis and metabolism of the tropane alkaloids. Planta Med 36:97–112 Leete E (1990) Recent development in the biosynthesis of the tropane alkaloids. Planta Med 56: 339–352
Martin-Tanguy J, Martin C, Paynot M, Rossin N (1988) Effect of hormone treatment on growth bud formation and free amine and hydroxycinnamoylputrescine levels in leaf explants of Nicotiana tabacum cultivated in vitro. Plant Physiol 88: 600–605
Martin-Tanguy J, Tepfer D, Paynot M, Burtin D, Heisler L, Martin C (1990) Inverse relationship between polyamine levels and the degree of phenotypic alteration induced by the root-inducing, left-hand transferred DNA from Agrobacterium rhizogenes. Plant Physiol 92: 912–918
Masgrau C, Altabella T, Farrâs R, Flores D, Thompson AJ, Besford RT, Tiburcio AF (1997) Inducible over-expression of oat arginine decarboxylase transgenic tobacco plants. Plant J 11: 465–473
McLauchlan WR, McKee RA, Evans DM (1993) The purification and immunocharacterization of N-methylputrescine oxidase from transformed root cultures of Nicotiana tabacum L. cv SC58. Planta 191: 440–445
Medina-Bolivar F, Flores HE (1995) Selection for hyoscyamine and cinnamoyl putrescine overproduction in cell and root cultures of Hyoscyamus muticus. Plant Physiol 108: 1553–1560
Molyneux RJ, Pan YT, Goldmann A, Tepfer DA, Elbein DA (1993) Calystegines: a novel class of alkaloid glycosidase inhibitors. Arch Biochem Biophys 304: 81–88
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassys with tobacco tissue cultures. Physiol Plant 15: 473–479
Nakajima K, Oshita Y, Kaya H, Yamada Y, Hashimoto T (1999) Structures and expression patterns of two tropinone reductase genes from Hyoscyamus niger. Biosci Biotechnol Biochem 63: 1756–1764
Nuutila AM, Lindqvist A-S, Kauppinen V (1997) Growth of hairy root cultures of strawberry (Fragaria x ananassa Duch.) in three different types of bioreactors. Biotech Tech 11: 363366
Oksman-Caldentey K-M (1986) Production of scopolamine and hyoscyamine in some solanaceous plants and cell cultures. Acta Pharm Fenn 95: 49–58
Oksman-Caldentey K-M (1987) Scopolamine and hyoscyamine production by plants and cell cultures of Hyoscyamus muticus. PhD Thesis, University of Helsinki, Helsinki, Finland
Oksman-Caldentey K-M, Hiltunen R (1996) Transgenic crops for improved pharmaceutical products. Field Crops Res 45: 57–69
Oksman-Caldentey K-M, Strauss A (1986) Somaclonal variation of scopolamine content in protoplast-derived cell cultures clones of Hyoscyamus muticus. Planta Med 52: 6–12
Oksman-Caldentey K-M, Vuorela H, Strauss A, Hiltunen R (1987a) Variation in the tropane alka- loid content of Hyoscyamus muticus plants and cell culture clones. Planta Med 53: 349–354
Oksman-Calentey K-M, Vuorela H, Isenegger M, Strauss A, Hiltunen R (1987b) Selection for high tropane alkaloid content in Hyoscyamus muticus plants. Plant Breed 99: 318–326
Oksman-Caldentey K-M, Strauss A, Hiltunen R (1987c) Optimization of some parameters in cultivating scopolamine-producing cells of Hyoscyamus muticus. Acta Pharm Fenn 96: 91–99
Oksman-Caldentey K-M, Kivelä O, Hiltunen R (1991) Spontaneous shoot organogenesis and plant regeneration from hairy root cultures of Hyoscyamus muticus. Plant Sci 78: 129–136
Oksman-Caldentey K-M, Sevôn N, Vanhala L, Hiltunen R (1994) Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus. Plant Cell Tissue Organ Cult 38: 263–272
Palazbn J, Cusick-) RM, Roig C, Pinnol MT (1997) Effect of rol genes from Agrobacterium rhizogenes TL-DNA on nicotine production in tobacco root cultures. Plant Physiol Biochem 35: 155–162
Pannuri S, Ramakrishna R, McNeill D, Curtis WR (1993) Interpreting the role of phosphorus and growth rate in enhanced fungal induction of sesquiterpenes from Hyoscyamus muticus root cutures. Appl Microbiol Biotechnol 38: 550–555
Parthier B (1990) Jasmonates: hormonal regulators or stress factors in leaf senescence? J Plant Growth Regul 9: 1–7
Payne J, Hamill JD, Robins RJ, Rhodes MJC (1987) Production of hyoscyamine by “hairy root” cultures of Datura stramonium. Planta Med 53: 474–478
Ramakrishna G, Signs MW (1993) Reversed-phase liquid chromatographic isolation of lubimin and solavetivone from Hyoscyamus muticus “hairy” root cultures. J Chromatogr 657: 440–444
Rhodes MJC (1994) Physiological roles for secondary metabolites in plants: some progress, many outstanding problems. Plant Mol Biol 24: 1–20
Rhodes MJC, Robins RJ, Hamill JD, Parr AJ, Hilton MG, Walton NJ (1990) Properties of transformed root cultures. In: Charlwood BV, Rhodes MJC (eds) Secondary products from plant tissue cultures. Clarendon Press, Oxford, pp 201–225
Rhodes MJC, Parr AJ, Giulietti A, Aird ELH (1994) Influence of exogenous hormones on the growth and secondary metabolite formation in transformed root cultures. Plant Cell Tissue Organ Cult 38: 143–151
Robins RJ, Parr AJ, Walton NJ, Rhodes MJC (1990) Factors regulating tropane-alkaloid production in a transformed root culture of Datura candida x D. aurea hybrid. Planta 181: 414–422
Robins RJ, Bent EG, Rhodes MJC (1991a) Studies on the biosynthesis of tropane alkaloids by Datura stramonium L. transformed root cultures. 3. The relationship between morphological integrity and alkaloid biosynthesis. Planta 185: 385–390
Robins RJ, Parr AJ, Walton NJ (19916) Studies on the biosynthesis of tropane alkaloids by Datura stramonium L. transformed root cultures. 2. On the relative contributions of L-arginine and L-ornithine to the formation of the tropane ring. Planta 183: 196–201
Robins RJ, Parr AJ, Bent EG, Rhodes MJC (1991c) Studies on the biosynthesis of tropane alkaloids by Datura stramonium L. transformed root cultures. 1. The kinetics of alkaloid production and the influence of feeding intermediate metabolites. Planta 183: 185–195
Robins RJ, Woolley JG, Ansarin M, Eagles J, Goodfellow B (1994a) Phenyllactic acid but not tropic acid is an intermediate in the biosynthesis of tropane alkaloids in Datura and Brugmansia transformed root cultures. Planta 194: 86–94
Robins RJ, Bachmann P, Woolley JG (1994b) Biosynthesis of hyoscyamine involves an intramolecular rearrangement of littorine. J Chem Soc Perkin Trans 1: 615–619
Robins RJ, Abraham TW, Parr AJ, Eagles J, Walton NJ (1997) The biosynthesis of tropane alkaloids in Datura stramonium: The identity of the intermediates between N-methyl-41pyrrolinium salt and tropinone. J Am Chem Soc 119: 10929–10934
Schaerer S, Pilet P-E (1991) Roots, explants and protoplasts from pea transformed with strains of Agrobacterium tumefaciens and rhizogenes. Plant Sci 78: 247–258
Severn N (1997) Tropane alkaloids in hairy roots and regenerated plants of Hyoscyamus muticus. PhD Thesis, University of Helsinki, Helsinki, Finland
Severn N, Hiltunen R, Oksman-Caldentey K-M (1992) Chitosan increases hyoscyamine content in hairy root cultures of Hyoscyamus muticus. Pharm Pharmacol Lett 2: 96–99
Severn N, Oksman-Caldeney K-M, Hiltunen R (1995) Efficient regeneration of plants from hairy root-derived protoplasts of Hyoscyamus muticus. Plant Cell Rep 14: 738–742
Severn N, Dräger B, Hiltunen R, Oksman-Caldentey K-M (1997) Characterization of transgenic plants derived from hairy roots of Hyoscyamus muticus. Plant Cell Rep 16: 605–611
Severn N, Hiltunen R, Oksman-Caldentey K-M (1998) Somaclonal variation in Agrobacterium transformed roots and protoplast-derived hairy root clones of Hyoscyamus muticus. Planta Med 64: 37–41
Shen WH, Petit A, Guern J, Tempé J (1988) Hairy roots are more sensitive to auxin than normal roots. Proc Natl Acad Sci 85: 3417–3421
Shen WH, Davioud E, David C, Barbier-Brygoo H, Tempé J, Guern J (1990) High sensitivity to auxin is a common feature of hairy roots. Plant Physiol 94: 554–560
Shimomura K, Sauerwein M, Ishimaru K (1991) Tropane alkaloids in adventitious and hairy root cultures of solanaceous plants. Phytochemistry 30: 2275–2278
Signs MW, Flores HE (1989) Elicitation of sesquiterpene phytoalexin biosynthesis in transformed root cultures of Hyoscyamus muticus. Plant Physiol 89 Suppl 135
Signs MW, Flores HE (1990) The biosynthetic potential of plant roots. Bioessays 12: 7–13
Singh G, Gavrelli J, Oakey JS, Curtis WR (1998) Interaction of methyl jasmonate, wounding and fungal elicitation during sesquiterpene induction in Hyoscyamus muticus in root cultures. Plant Cell Rep 17: 391–395
Subroto MA, Kwok KH, Hamill JD, Doran PM (1996) Coculture of genetically transformed roots and shoots for synthesis, translocation, and biotransformation of secondary metabolites. Biotechnol Bioeng 49: 481–494
Sun L-Y, Monneuse M-O, Martin-Tanguy J, Tepfer D (1991) Changes in flowering and accumulation of polyamines and hydroxycinnamic acid-polyamine conjugates in tobacco plants transformed by the rolA locus from the Ri TL-DNA of Agrobacterium rhizogenes. Plant Sci 80: 145–149
Tepfer D (1984) Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 37: 959–967
Tepfer D (1990) Genetic transformation using Agrobacterium rhizogenes. Physiol Plant 79: 140–146
Tepfer D, Damon J-P, Ben-Hayyim G, Pellegrineschi A, Burtin D, Martin-Tanguy J (1994) Control of root system architecture through chemical and genetic alterations of polyamine metabolism. In: Davis TD, Haissig BE (eds) Biology of adventitious root formation. Plenum Press, New York, pp 181–189
Tiburcio AF, Kaur-Sawhney R, Ingersoll RB, Galston AW (1985) Correlation between polyamines and pyrrolidine alkaloids in developing tobacco callus. Plant Physiol 78: 323–326
Tiburcio AF, Campos JL, Figueras X, Besford RT (1993) Recent advances in the understanding of polyamine functions during plant development. Plant Growth Regul 12: 331–340
Toivonen L (1993) Utilization of hairy root cultures for production of secondary metabolites. Biotechnol Prog 9: 12–20
Toivonen L, Rosenqvist H (1995) Establisment and growth characteristics of Glycyrrhiza glabra hairy root cultures. Plant Cell Organan an Cult 41: 249–258
Ueda, Miyamoto K, Hashimoto M (1996) Jasmonates promote abscission in bean petiole explants: its relationship to the metabolism of cell wall polysaccharides and cellulase activity. J Plant Growth Regul 15: 189–195
Vanhala L, Hiltunen R, Oksman-Caldentey K-M (1995) Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus. Plant Cell Rep 14: 236–240
Vanhala L, Eeva M, Lapinjoki S, Hiltunen R, Oksman-Caldentey K-M (1998) Effect of growth regulators on transformed root cultures of Hyoscyamus muticus. J Plant Physiol 153: 475–481
Watson MB, Emory KK, Piatak RM, Malmberg RL (1998) Arginine decarboxylase (polyamine synthesis) mutants of Arabidopsis thaliana exhibit altered root growth. Plant J 13: 231–239
Wilson PDG, Hilton MG, Meehan PTH, Waspe CR, Rhodes MJC (1990) The cultivation of transformed roots from laboratory to pilot plant. In: Nijkamp HJJ, van der Plas LHW, van Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht, pp 700–705
Yukimune Y, Hara Y, Yamada Y (1994) Tropane alkaloid production in root cultures of Duboisia myoporoides obtained by repeated selection. Biosci Biotechnol Biochem 58: 1443–1446
Yun D-J, Hashimoto T, Yamada Y (1992) Metabolic engineering of medicinal plants: Transgenic
Atropa belladonna with an improved alkaloid composition. Proc Nat! Acad Sci USA 89: 11799–11803
Zenk MH, EL-Shagi H, Arens H, Stöckigt J, Weiler EW, Deus B (1977) Formation of the indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In: Barz W, Reinhard E, Zenk MH (eds) Plant tissue culture and its biotechnological applications. Springer, Berlin Heidelberg New York, pp 27–43
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Sevón, N., Biondi, S., Bagni, N., Oksman-Caldentey, KM. (2001). Transgenic Hyoscyamus muticus (Egyptian henbane). In: Bajaj, Y.P.S. (eds) Transgenic Crops III. Biotechnology in Agriculture and Forestry, vol 48. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10603-7_13
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