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Root induction on several Solanaceae species by Agrobacterium rhizogenes and the determination of root tropane alkaloid content

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Abstract

Using Agrobacterium rhizogenes, roots were induced on explants of 24 different Solanaceae species and established as in vitro cultures. Some of the root clones produced tropane alkaloids at levels similar to roots of the corresponding intact plants and maintained these levels over several passages.

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Abbreviations

ELISA:

Enzyme linked immuno sorbent assay

IBA:

Indole butyric acid

LS-medium:

Medium after Linsmaier and Skoog (1965)

QNB:

Quinuclidinyl benzilate

RIA:

Radio immuno assay

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Communicated by P. J. King

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Knopp, E., Strauss, A. & Wehrli, W. Root induction on several Solanaceae species by Agrobacterium rhizogenes and the determination of root tropane alkaloid content. Plant Cell Reports 7, 590–593 (1988). https://doi.org/10.1007/BF00272765

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  • DOI: https://doi.org/10.1007/BF00272765

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