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Regeneration of Plants from Protoplasts of Hordeum vulgare (Barley)

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Plant Protoplasts and Genetic Engineering VII

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 38))

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Abstract

Barley (Hordeum vulgare L.) has been well known as a brewing material since early times, but today, a greater quantity is consumed for animal feed. Barley, originating from Near East Asia, has been distributed and adapted to a wider range of environmental conditions than any other cereal (Nilan and Ullrich 1993). Based on the figures presented by USDA (1995) the former USSR is the leading producing region, followed by the EU and North America.

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References

  • Abe S, Takeda J (1986) Possible involvement of calmodulin and the cytoskeleton in electorfusion of plant protoplasts. Plant Physiol 81: 1151–1155

    Article  PubMed  CAS  Google Scholar 

  • Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18: 659–668

    Google Scholar 

  • Daeter W, Hartung W (1993) The permeability of the epidermal cell plasma membrane of barley leaves to abscisic acid. Planta 191: 41–17

    Article  CAS  Google Scholar 

  • Diaz I, Carbonero P (1992) Isolation of protoplasts from developing barley endosperm: a tool for transient expression studies. Plant Cell Rep 10: 595–598

    Article  Google Scholar 

  • Diez KJ, Schramm M, Betz M, Busch H, Dürr C, Martionoia E (1992) Characterization of the epidermis from barley primary leaves. I. Isolation of epidermal protoplasts. Planta 187: 425–430

    Google Scholar 

  • Funatsuki H, Kihara M (1994) Influence of primary callus induction conditions on the establishment of barley cell suspensions yielding regenerable protoplasts. Plant Cell Rep 13: 551–555

    Article  CAS  Google Scholar 

  • Funatsuki H, Lörz H, Lazzeri PA (1992) Use of feeder cells to improve barley protoplast culture and plant regeneration. Plant Sci 85: 179–187

    Article  Google Scholar 

  • Funatsuki H, Müller E, Lörz H, Lazzeri PA (1994) Production of somatic hybrid calli between Hordeum vulgare L. and Hordeum bulbosum L. J Plant Physiol 144: 251–254

    Article  Google Scholar 

  • Funatsuki H, Kuroda H, Kihara H, Lazzeri PA, Müller E, Lörz H, Kishinami I (1995) Fertile transgenic barley generated by direct DNA transfer to protoplasts. Theor Appl Genet 91: 707–712

    Article  CAS  Google Scholar 

  • Golds TJ, Babczinsky J, Mordhorst AP, Koop HU (1994) Protoplast preparation without centrifugation: plant regeneration of barley (Hordeum vulgare L.). Plant Cell Rep 13: 188–192

    Article  CAS  Google Scholar 

  • Hoekstra S, van Zijderveld MH, Hidekamp F, van der Mark F (1993) Microspore culture of Hordeum vulgare L.: the influence of density and osmolality. Plant Cell Rep 12: 661–665

    Article  Google Scholar 

  • Holm PB, Knunden S, Mouritzen P, Negri D, Olsen FL, Roué C (1994) Regeneration of fertile barley plants from mechanically isolated protoplasts of the fertilized egg cell. Plant Cell 6: 531–543

    PubMed  CAS  Google Scholar 

  • Jacobsen JV, Zwar JA, Chandler PH (1985) Gibberellic acid responsive protoplasts from mature aleurone of Himalaya barley. Planta 163: 430–438

    Article  CAS  Google Scholar 

  • Jähne A, Lazzeri PA, Jäger-Güssen M, Lörz H (1991a) Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley (Hordeum vulgare L.). Theor Appl Genet 82: 74–80

    Article  Google Scholar 

  • Jähne A, Lazzeri PA, Lörz H (199 lb) Regeneration of fertile plants from protoplasts derived from embryogenic cell suspensions of barley (Hordeum vulgare L.). Plant Cell Rep 10: 1–6

    Google Scholar 

  • Junker B, Zimney J, Rührs R, Lörz H (1987) Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptake. Plant Cell Rep 6: 329–332

    Article  CAS  Google Scholar 

  • Karp A, Lazzeri PA (1992) Regeneration, stability and transformation in barley. In: Shewry R (ed) Barley: genetics, molecular biology and biotechnology. CAB International, Oxford, p 519–572

    Google Scholar 

  • Kihara M, Funatsuki H (1994) Fertile plant regeneration from barley (Hordeum vulgare L.) protoplasts isolated from long-term suspension culture. Breed Sci 44: 157–160

    Google Scholar 

  • Kihara M, Funatsuki H (1995) Fertile plant regeneration from barley (Hordeum vulgare L.) protoplasts isolated from primary calluses. Plant Sci 106: 115–120

    Article  CAS  Google Scholar 

  • Koblitz H (1976) Isolierung und Kultivierung von Protoplasten aus Kalluskulturen der Gerste. Biochem Physiol Pflanz 170: 287–293

    Google Scholar 

  • Koblitz H (1986) Barley (Hordeum vulgare L.): establishment of cultures and the regeneration of plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 2. Crops I. Springer, Berlin Heidelberg New York, pp 181–203

    Google Scholar 

  • Kuhlmann U, Foroughi-Wehr B (1989) Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep 6: 191–193

    Google Scholar 

  • Lazzeri PA, Lörz H (1990) Regenerable suspension and protoplast cultures of barley and stable transformation via DNA uptake into protoplasts. In: Lycett GW, Grierson D (eds) Genetic engineering of crop plants. Butterworth, London, pp 231–238

    Google Scholar 

  • Lazzeri PA, Brettschneider, Lührs R, Lörz H (1991) Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts. Theor Appl Genet 81: 437–444

    Article  Google Scholar 

  • Lazzeri PA, Jähne A, Lörz H (1992) Culture, regeneration and transformation of barley protoplasts. In: Lindsey K (ed) Plant tissue culture manual: fundamentals and applications, part B10. Kluwer, Dordrecht, pp 1–11

    Google Scholar 

  • Lee BT, Murdoch K, Topping J, Kreis M, Jones MGK (1989) Transient expression in aleurone protoplasts isolated from developing caryopses of barley and wheat. Plant Mol Biol 13: 21–29

    Article  PubMed  CAS  Google Scholar 

  • Lee BT, Murdoch K, Topping J, Jones MGK, Kreis M (1991) Transient expression of foreign genes introduced into barley endosperm protoplasts by PEG-mediated transfer into intact endosperm tissue by microprojectile bombardment. Plant Sci 78: 237–246

    Article  CAS  Google Scholar 

  • Lindberg S (1995) In-situ determination of interacellular concentrations of K± in barley (Hordeum vulgare L. cv. Kara) using the K+-binding fluorescent dye benzofuran isophthalate. Planta 195: 525–529

    Article  CAS  Google Scholar 

  • Logue SJ, Giles LC, Sparrow DHB (1993) Genotype and environment strongly influence barley anther culture response using Australian genotypes. Aust J Bot 41: 227–236

    Article  Google Scholar 

  • Lührs R, Lörz H (1987) Plant regeneration in vitro from embryogenic cultures of spring-and winter-type barley (Hordeum vulgare L.) varieties. Theor Appl Genet 75: 16–25

    Article  Google Scholar 

  • Lührs R, Lörz H (1988) Initiation of morphogenic cell suspension and protoplast cultures of barley(Hordeum vulgare L.). Planta 175: 71–81

    Article  Google Scholar 

  • Mordhorst AP, Lörz H (1992) Electrostimulated regeneration of plantlets from protoplasts derived from cell suspension of barley (Hordeum vulgare L.). Physiol Plant 85: 289–294

    Article  CAS  Google Scholar 

  • Mordhorst AP, Lörz H (1993) Embryogenesis and development of isolated barley (Hordeum vulgare L.) microspores are influenced by the amount and composition of nitrogen sources in culture media. J Plant Physiol 142: 485–492

    Article  CAS  Google Scholar 

  • Müller AJ, Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161: 67–76

    Article  Google Scholar 

  • Nilan RA, Ullrich SE (1993) Barley: Taxonomy, origin, distribution, production, genetics, and breeding. In: McGregor AW, Bhatty RS (eds) Barley: chemistry and technology. Am Assoc Cereal Chem St Paul, pp 1–29

    Google Scholar 

  • Olsen (1987) Induction of microspore embryogenesis in cultured anthers of Hordeum vulgare. The effect of ammonium nitrate, glutamine and asparagine as nitrogen sources. Carlsberg Res Commun 52: 393–404

    Article  CAS  Google Scholar 

  • Salmenkallio M, Hannus R, Teen TH, Kauppinen V (1990) Regulation of d-amylase promoter by gibberellic acid and abscisic acid in barley protoplasts transformed by electroporation. Plant Cell Rep 9: 352–355

    Article  CAS  Google Scholar 

  • Salmenkallio-Marttila M, Kauppinen V (1995) Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.). Plant Cell Rep 14: 253–256

    Article  CAS  Google Scholar 

  • Salmenkallio-Marttila M, Aspegren K, Akerman S, Kurten U, Mannonen L, Ritala A, Teeri TH, Kauppinen V (1995) Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts. Plant Cell Rep 15: 301–304

    Article  CAS  Google Scholar 

  • Sato N, Kuroda H, Kihara M, Kishimami I (1991) Plant regeneration from protoplast-derived callus of barley (Hordeum vulgare L.). In: Munck L (ed) Barley genetics VI, vol I. Proc 6th Int Barley Genet Symp. Munksgard International, Copenhagen, pp 217–219

    Google Scholar 

  • Snape JW, Laurie DA, Leitch IJ, Heslop-Harrison JS (1992) Prospects in barley cytogenetics. In: Munck L (ed) Barley genetics VI, vol II. Proc 6th Int Barley Genet Symp. Munksgard International Copenhagen, pp 833–848

    Google Scholar 

  • Somers DA, Narayanan KR, Kleinhofs A, Cooper-Bland S, Cocking EC (1986) Immunological evidence for transfer of the barley nitrate reductase structural gene to Nicotiana tabacum by protoplast fusion. Mol Gen Genet 204: 296–301

    Article  CAS  Google Scholar 

  • Stirn S, Hopstock A, Lörz H (1994) Bioreactor cultures of embryogenic suspensions of barley (Hordeum vulgare L.) and maize ( Zea mays L. ). J Plant Physiol 144: 209–214

    Google Scholar 

  • Takahashi S, Kaneko T (1987) Protoplast isolation and culture of barley. In: Yasuda S (ed) Barley genetics V. Proc 5th Int Barley Genet Symp. Maruzen, Okayama, pp 557–562

    Google Scholar 

  • USDA (1995) Grain: world markets and trade. February, pp 31–33

    Google Scholar 

  • Von Bothmer R, Flink H, Jacobsen N, Kotimaki M, Landstrom T (1983) Interspecific hybridization with cultivated barley (Hordeum vulgare L.). Hereditas 99: 29–244

    Google Scholar 

  • Wang XH, Lörz H (1994) Plant regeneration from protoplasts of wild barley ( Hordeum murinum L. ). Plant Cell Rep 13: 139–144

    Google Scholar 

  • Yan Q, Zhang X, Shi J, Li J (1990) Green plant regeneration from protoplasts of barley ( Hordeum vulgare L. ). Kexue Tongbao 35: 1581–1583

    Google Scholar 

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© 1996 Springer-Verlag Berlin Heidelberg

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Funatsuki, H., Kihara, M., Takahashi, S., Lazzeri, P.A., Lörz, H. (1996). Regeneration of Plants from Protoplasts of Hordeum vulgare (Barley). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering VII. Biotechnology in Agriculture and Forestry, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09368-9_6

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  • DOI: https://doi.org/10.1007/978-3-662-09368-9_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08240-5

  • Online ISBN: 978-3-662-09368-9

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