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Genetics and Molecular Biology of Methylotrophic Yeasts

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Yeasts in Natural and Artificial Habitats

Abstract

At least 31 yeasts are able to utilize methanol as a sole carbon source (J.A. Barnett, pers. comm.). There is now much interest in these organisms for basic biological and ecological studies and practical applications. The development of genetic tools is advanced enough that three species are amenable to genetic analysis and recombinant DNA systems for them are known. Details of the methylotrophic yeasts are available (Table 14.1), including reviews on their genetics and molecular biology (Cregg 1986; Sibirny et al. 1988; Sudbery and Gleeson 1989). We discuss here current knowledge of fundamental and biotechnological problems.

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References

  • Barnett JA, Paine RW, Yarrow D (1990) Yeasts. Characteristics and identification. Cambridge University Press, Cambridge

    Google Scholar 

  • Beburov MY, Zlochewsky ML, Michailover VM, Semenova VD, Gracheva VD, Lahtchev K (1990) Transformation of methylotrophic yeast Hansenula polymorpha with linear DNA fragments. Yeast 6: S118

    Google Scholar 

  • Berardi E, Thomas DY (1990) An effective transformation method for Hansenula polymorpha. Curr Genet 18: 169–170

    Article  CAS  Google Scholar 

  • Berardi E, Meloni MG, Bonomo RP, Zoroddu MA (1990) Electron paramagnetic resonance study of retention of chromium (III), chromate or dichromate(VI) and copper(II) ions by thermotolerant Hoansenula polymorpha. J Chem Soc Faraday Trans 86: 2579–2582

    Article  CAS  Google Scholar 

  • Borst P (1989) Peroxisome biogenesis revisited. Biochem Biophys Acta 1008: 1–13

    Article  PubMed  CAS  Google Scholar 

  • Bruinenberg PG, Evers M, Waterham HR, Kuipers J, Amberg AC, Ab G (1989) Cloning and sequencing of the peroxisomal amine oxidase gene from Hansenula polymorpha. Biochim Biophys Acta 1008: 157–167

    Article  PubMed  CAS  Google Scholar 

  • Bruinenberg PG, Blaauw M, Kazemier B, Ab G (1990) Cloning and sequencing of the malate sythase gene from Hansenula polymorpha. Yeast 6: 245–254

    Article  PubMed  CAS  Google Scholar 

  • Brul S, Westerveld A, Strijland A, Wanders RJA, Schram AW, Heymans HSA, Schutgens RBH, Van den Bosch H, Tager JM (1988) Genetic heterogeneity in the cerebrohepatorenal ( Zellweger) syndrome and other inherited disorders with a generalized impairment of peroxisomal functions. J Clin Invest 81: 1710–1715

    Google Scholar 

  • Buckholtz RG, Gleeson MAG (1991) Yeast systems for the commercial production of heterologous proteins. Bio/Technology 9: 1067–1972

    Article  Google Scholar 

  • Clare JJ, Rayment FB, Ballantine SP, Sreekrishna K, Romanos MA (1991) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Bio/Technology 9: 455–460

    Article  PubMed  CAS  Google Scholar 

  • Cregg JM (1986) Genetics of methylotrophic yeasts. In: Van Verseveld HW, Duine JA (eds) Microbial growth on Cl compounds. Proc 5th Int Symp. Martinus Nijhoff, Dordrecht, pp 158–167

    Google Scholar 

  • Cregg JM, Barringer KJ, Hessler AY, Madden KR (1985) Pichia pastoris as a host system for transformations. Mol Cell Biol 5: 3376–3385

    Google Scholar 

  • Cregg JM, Tschopp JF, Stillman C, Siegel R, Akong M, Craig WS, Buckholz RG, Madden KR, Kellaris PA, Davis GR, Smiley BL, Cruze J, Torregrossa R, Velicelebi G, Thill GP (1987) High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris. Bio/Technology 5: 479–485

    Article  CAS  Google Scholar 

  • Cregg JM, Madden KR, Barringer KJ, Thill GP, Stillman CA (1989) Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol Cell Biol 9: 1316–1323

    PubMed  CAS  Google Scholar 

  • Cregg JM, van der Klei IJ, Sulter GJ, Veenhuis M, Harder W (1990) Peroxisome-deficient mutants of Hansenula polymorpha. Yeast 6: 87–97

    Article  CAS  Google Scholar 

  • De Hoop MJ, Asgeirsdottir S, Blaauw M, Veenhuis M, Cregg J, Gleeson M, Ab G (199la) Mutations in the FAD-binding fold of alcohol oxidase from Hansenula polymorpha. Protein Engin 4: 82 1829

    Google Scholar 

  • De Hoop MJ, Cregg J, Keizer-Gunnink I, Sjollema K, Veenhuis M, Ab G (1991b) Overexpression of alcohol oxidase in Pichia pastoris. FEBS Lett 291: 299–302

    Article  PubMed  Google Scholar 

  • De Hoop MJ, Valkema R, Kienhuis CBM, Hoyer MA, Ab G (1992) The peroxisomal import signal of amine oxidase from the yeast Hansenula polymorpha is not universal. Yeast 8: 243–252

    Article  PubMed  Google Scholar 

  • De Koning W, Gleeson MAG, Harder W, Dijkhuizen L (1987) Regulation of methanol metabolism in the yeast Hansenula polymorpha. Isolation and characterization of mutants blocked in methanol assimilatory enzymes. Arch Microbiol 147: 375–382

    Google Scholar 

  • De Koning W, Bonting K, Harder W, Dijkhuizen L (1990a) Classical transketolase functions as the formaldehyde-assimilating enzyme during growth of a dihydroxyacetone synthase-negative mutant of the methylotrophic yeast Hansenula polymorpha on mixtures of xylose and methanol in continuous cultures. Yeast 6: 117–125

    Article  Google Scholar 

  • De Koning W, Weusthuis RA, Harder W, Dijkhuizen L (1990b) Methanol-dependent production of dihydroxyacetone and glycerol by mutants of the methylotrophic yeast Hansenula polymorpha blocked in dihydroxyacetone kinase and glycerol kinase. Glycerol and dihydroxyacetone production. Appl Microbiol Biotechnol 32: 693–698

    Google Scholar 

  • De Koning W, Weusthuis RA, Harder W, Dijkhuizen L (1990c) Metabolic regulation in the yeast Hansenula polymorpha. Growth of dihydroxyacetone kinase/glycerol kinase-negative mutants on mixtures of methanol and xylose in continuous cultures. Yeast 6: 107–115

    Google Scholar 

  • De Roubin MR, Bastien L, Shen SH, Groleau D (1991) Fermentation study for the production of hepatitis B virus pre-S2 antigen by the methylotrophic yeast Hansenula polymorpha. J Ind Microbiol 8: 147–156

    Article  PubMed  Google Scholar 

  • Didion T, Roggenkamp R (1992a) Deficiency of peroxisome assembly in a mutant of the methylotrophic yeast Hansenula polymorpha. Curr Genet 17: 113–117

    Article  Google Scholar 

  • Didion T, Roggenkamp R (1992b) Targeting signal of the peroxisomal catalase in the methylotrophic yeast Hansenula polymorpha. FEBS Lett 303: 113–116

    Article  PubMed  CAS  Google Scholar 

  • Digan ME, Lair SV, Brierly RA, Siegel RS, Williams ME, Ellis SB, Kellaris PA, Provow SA, Craig WS, Velicelebi G, Harpold MM, Thill GP (1989) Continuous production of a novel lysozyme via secretion from the yeast, Pichia pastoris. Bio/Technology 7: 160–164

    Article  CAS  Google Scholar 

  • Distel B, Veenhuis M, Tabak HF (1987) Import of alcohol oxidase into peroxisomes of Saccharomyces cerevisiae. EMBO J 6: 3111–3116

    PubMed  CAS  Google Scholar 

  • Distel B, Van der Ley I, Veenhuis M, Tabak HF (1988) Alcohol oxidase expressed under nonmethylotrophic condition is imported, assembled and enzymatically active in peroxisomes of Hansenula polymorpha. J Cell Biol 107: 1669–1675

    Article  PubMed  CAS  Google Scholar 

  • Douma AC, Veenhuis M, Waterham HR, Harder W (1988) Immunocytochemical demonstration of the peroxisomal ATPase of yeasts. Yeast 6: 45–51

    Article  Google Scholar 

  • Eggeling L, Sahm (1980) Regulation of alcohol oxidase synthesis in Hansenula polymorpha: oversynthesis during growth on mixed substrates and induction by methanol. Arch Microbiol 127: 119–124

    CAS  Google Scholar 

  • Ellis SB, Brust PF, Koutz PJ, Waters AF, Harpold MM, Gingeras TR (1985) Isolation of alcohol oxidase and two other methanol-regulatable genes from the yeast Pichia pastoris. Mol Cell Biol 5: 1111–1121

    PubMed  CAS  Google Scholar 

  • Erdmann R, Veenhuis M, Mertens D, Kunau WH (1989) Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 86: 5419–5423

    Article  PubMed  CAS  Google Scholar 

  • Erdmann R, Wiebel FF, Flessau A, Rytka J, Beyer A, Frohlich KU, Kunau WH (1991) PAS1, a yeast gene required for peroxisome biogenesis, encodes a member of a novel family of putative ATPases. Cell 64: 499–510

    Article  PubMed  CAS  Google Scholar 

  • Faber KN, Swaying GJ, Faber F, Ab G, Harder W, Veenhuis M, Haima P (1992) Chromosomal targeting of replicating plasmids in the yeast Hansenula polymorpha. J Gen Microbiol (in press)

    Google Scholar 

  • Fellinger AJ, Verbakel JMA, Veale RA, Sudbery PE, Bom IJ, Overbeeke N, Verrips CY (1991) Expression of the a-galactosidase from Cyamopsis tetragonoloba (guar) by Hansenula polymorpha. Yeast 7: 463–473

    Article  PubMed  CAS  Google Scholar 

  • Gellissen G, Janowicz ZA, Merkelbach A, Piontek M, Keup P, Weydemann U, Hollenberg CP, Strasser AWM (1991) Heterologous gene expression in Hansenula polymorpha: efficient secretion of glucoamylase. Bio/Technology 9: 291–295

    Article  PubMed  CAS  Google Scholar 

  • Gellissen G, Melber K, Janowicz A, Dahlems UM, Weydemann U (1992) Heterologous protein production in yeast. Antonie Leeuwenhoek 62: 79–93

    Article  PubMed  CAS  Google Scholar 

  • Giuseppin MLF (1988) PhD Thesis, University of Delft, Delft

    Google Scholar 

  • Giuseppin MLF, Van Eijk HMJ, Bes BCM (1988a) Molecular regulation of methanol oxidase activity in continuous cultures of Hansenula polymorpha. Biotechnol Bioeng 32: 577–583

    Article  PubMed  CAS  Google Scholar 

  • Giuseppin MLF, Van Eijk HMJ, Verduyn C, Bante I, Van Dijken JP (1988b) Production of catalase-free alcohol oxidase by Hansenula polymorpha. Appl Microbiol Biotechnol 28: 14–19

    CAS  Google Scholar 

  • Gleeson MA, Sudbery PE (1988a) The methylotrophic yeasts. Yeast 4: 1–15

    Article  CAS  Google Scholar 

  • Gleeson MA, Sudbery PE (1988b) Genetic analysis in the methylotrophic yeast Hansenula polymorpha. Yeast 4: 293–303

    Article  CAS  Google Scholar 

  • Gleeson MA, Waites MJ, Sudbery PE (1984) Development of techniques for genetic analysis in the methylotrophic yeast Hansenula polymorpha. In: Crawford RL, Hanson RS (eds) Microbial growth on Cl compounds. Proc 4th Int Symp. American Society for Microbiology. Washington DC, pp 228–235

    Google Scholar 

  • Gleeson MA, Ortori GS, Sudbery PE (1986) Transformation of the methylotrophic yeast Hansenula polymorpha. J Gen Microbiol 132: 3459–3465

    CAS  Google Scholar 

  • Godecke A, Veenhuis M, Roggenkamp R, Janowicz ZA, Hollenberg CP (1989) Biosynthesis of the peroxisomal dihydroxyacetone synthase from Hansenula polymorpha in Saccharomyces cerevisiae induces growth but not proliferation of peroxisomes. Curr Genet 16: 13–20

    Article  PubMed  CAS  Google Scholar 

  • Goodman TM, Maher J, Silver PA, Pacifico A, Sanders D (1986) The membrane proteins of the methanol-induced peroxisomes of Candida boidinii. J Biol Chem 261: 3464–3468

    PubMed  CAS  Google Scholar 

  • Gould SJ, Keller GA, Schneider M, Howell SH, Garrard LJ, Goodman JM, Distel B, Tabak H, Subramani S (1990) Peroxisomal protein import is conserved between yeast, plants, insects and mammals. EMBO J 9: 85–90

    PubMed  CAS  Google Scholar 

  • Gould SJ, McCollum D, Spong AP, Heyman JA, Subramani S (1992) Development of the yeast Pichia pastoris as a model organism for a genetic and molecular analysis of peroxisome assembly. Yeast 8: 613–628

    Article  PubMed  CAS  Google Scholar 

  • Hansen H, Roggenkamp R (1989) Functional complementation of catalase-defective peroxisomes in a methylotrophic yeast by import of catalase A from Saccharomyces cerevisiae. Eur J Biochem 184: 173–179

    Article  PubMed  CAS  Google Scholar 

  • Hijikata M, Ishii N, Kagamiyama H, Osumi T, Hashimoto T (1987) Structural analysis of cDNA for rat peroxisomal 3-ketoacyl-CoA thiolase. J Biol Chem 262: 8151–8158

    PubMed  CAS  Google Scholar 

  • Hohfeld J, Veenhuis M, Kunau WH (1991) PASS, a Saccharomyces cerevisiae gene encoding a peroxisomal integral membrane protein essential for peroxisome biogenesis. J Cell Biol 114: 1167–1178

    Article  PubMed  CAS  Google Scholar 

  • Janowicz ZA, Eckart MR, Drewke C, Roggenkamp RO, Hollenberg CP (1985) Cloning and characterization of the DAS gene encoding the major methanol assimilatory enzyme from the methyltrophic yeast Hansenula polymorpha. Nucleic Acids Res 13: 3043–3062

    Article  PubMed  CAS  Google Scholar 

  • Janowicz ZA, Melber K, Merckelbach A, Jacobs E, Harford N, Comberbach M, Hollenberg CP (1991) Simultaneous expression of the S and L surface antigens of hepatitis B, and formation of mixed particles in the methylotrophic yeast Hansenula polymorpha. Yeast 7: 431–443

    Article  PubMed  CAS  Google Scholar 

  • Kato N, Kobayashi H, Shimao M, Sakasawa C (1986) Dihydroxyacetone production from methanol by a dihydroxyacetone kinase-deficient mutant of Hansenula polymorpha. Appl Microbiol Biotechnol 23: 180–186

    Article  CAS  Google Scholar 

  • Komagata K (1991) Systematics of methylotrophic yeasts. In: Goldberg I, Stefan Rokem J (eds) Biology of methylotrophs. Butterworth-Heinemann, Boston, pp 25–37 (Biotechnology series, vol 18 )

    Google Scholar 

  • Koutz P, Davis GR, Stillman C, Barringer K, Cregg J, Thill G (1989) Structural comparison of the Pichia pastoris alcohol oxidase genes. Yeast 5: 167–177

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP (1984) Synonymy of the yeast genera Hansenula and Pichia demonstrated through comparisons of deoxyribonucleic acid relatedness. Antonie Leeuwenhoek 50: 209–217

    Article  PubMed  CAS  Google Scholar 

  • Lahtchev K, Penkova R, Ivanova V, Tuneva D (1992) Genetic analysis of methylotrophic yeast Candida boidinii PLD1. Antonie Leeuwenhoek 61: 185–194

    Article  PubMed  CAS  Google Scholar 

  • Lazarow PB, Moser HW (1989) Disorders of peroxisome biogenesis. In: Scriver CR, Beaudet AI, Sly WS, Valle D (eds) The metabolic basis of inherited disease, 5th edn, McGraw-Hill, New York, pp 14791509

    Google Scholar 

  • Ledeboer AM, Edens L, Maat J, Visser C, Bos JW, Verrips CT, Janowicz ZA, Eckart M, Roggenkamp R, Hollengerg CP (1985) Molecular cloning and characterization of a gene coding for methanol oxidase in Hansenula polymorpha. Nucleic Acids Res 13: 3063–3082

    Article  PubMed  CAS  Google Scholar 

  • Lim WJ, Tani Y (1988) Production of L-methionine-enriched cells of a mutant derived from a methylotrophic yeast, Candida boidinii. J Ferment Technol 66: 643–647

    Article  CAS  Google Scholar 

  • Liu H, Tan X, Veenhuis M, McCollum D, Cregg JM (1992) An efficient screen for peroxisome-deficient mutants of Pichia pastoris. J Bacteriol 174: 4943–4951

    PubMed  CAS  Google Scholar 

  • Moser HW, Bergin A, Cornblath D (1991) Peroxisomal disorders. Can J Biochem Cell Biol 69:463–474 Nicolay K, Veenhuis M, Douma AC, Harder W (1987) An 31P NMR study of the internal pH of yeast peroxisomes. Arch Microbiol 147: 37–41

    Google Scholar 

  • Orr-Weaver TL, Szostak JW, Rothstein RJ (1983) Genetic applications of yeast transformation with linear and gapped plasmids. In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology, vol 101. Academic Press, New York, pp 228–245

    Google Scholar 

  • Osumi T, Fujiki Y (1990) Topogenesis of peroxisomal proteins. BioEssays 12:217–222 Ratner M (1989) Protein expression in yeast. Bio/Technology 7: 1129–1133

    Google Scholar 

  • Roa M, Blobel B (1983) Biosynthesis of peroxisomal enzymes in the methylotrophic yeast Hansenula polymorpha. Proc Nati Acad Sci USA 80: 6872–6876

    Article  CAS  Google Scholar 

  • Roggenkamp R, Janowicz ZA, Stanikowski B, Hollenberg CP (1984) Biosynthesis and regulation of the peroxisomal methanol oxidase from the methylotrophic yeast Hansenula polymorpha. Mol Gen Genet 194: 489–493

    Article  PubMed  CAS  Google Scholar 

  • Roggenkamp R, Hansen H, Eckart M, Janowicz Z, Hollenberg CP (1986) Transformation of the methylotrophic yeast Hansenula polymorpha by autonomous replication and integration vectors. Mol Gen Genet 202: 302–308

    Article  CAS  Google Scholar 

  • Roggenkamp R, Didion, T, Kowalik KV (1989) Formation of irregular giant peroxisomes by overproduction of the crystalloid core protein methanol oxidase in the methylotrophic yeast Hansenula polymorpha. Mol Cell Biol 9: 988–994

    PubMed  CAS  Google Scholar 

  • Romanos MA, Scorer CA, Clare JJ (1992) Foreign gene expression in yeast: a review. Yeast 8: 423–488

    Article  PubMed  CAS  Google Scholar 

  • Sakai Y, Tani Y (1992) Directed mutagenesis in an ascosporogenous methyltrophic yeast: cloning, sequencing and one-step gene disruption of the 3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to derive double auxotrophic marker strains. J Bacteriol 174: 5988–5993

    PubMed  CAS  Google Scholar 

  • Sakai Y, Kazarimoto T, Tani Y (1991) Transformation system for an asporogenous methylotrophic yeast, Candida boidinii: cloning the orotidine-5’-phosphate decarboxylase gene (URA3), isolation of uracil auxotrophic mutants, and use of the mutants for integrative transformation. J Bacteriol 173: 7458–7463

    PubMed  CAS  Google Scholar 

  • Shen SH, Bastien L, Nguyen T, Fung M, Slilaty SN (1989) Synthesis and secretion of hepatitis B middle surface antigen by the methylotrophic yeast Hansenula polymorpha. Gene 84: 303–309

    Article  PubMed  CAS  Google Scholar 

  • Shimozawa N, Tsukamoto T, Suzuki Y, Orii T, Shirayoshi Y, Mori T, Fujiki Y (1992) A human gene responsible for Zellweger syndrome that affects peroxisome assembly. Science 255: 1132–1134

    Article  PubMed  CAS  Google Scholar 

  • Sibirny AA, Titorenko VI, Efremov BD, Tolstorukov II (1987) Multiplicity of mechanisms of carbon catabolite repression involved in the synthesis of alcohol oxidase in the methylotrophic yeast Pichia pin us. Yeast 3: 233–241

    Article  Google Scholar 

  • Sibirny AA, Titorenko VI, Gonchar MV, Ubiyvovk VM, Ksheminskaya GP, Vitvitskaya OP (1988) Genetic control of methanol utilization in yeasts. J Basic Microbiol 28: 293–319

    Article  PubMed  CAS  Google Scholar 

  • Sibirny AA, Titorenko VI, Teslyar GE, Petrushko VI, Kucher MM (1991) Methanol and ethanol utilization in methylotrophic yeast Pichia pinus wild-type and mutant strains. Arch Microbiol 156: 455462

    Google Scholar 

  • Sierkstra LN, Verbaki JMA, Verrips CT (1991) Optimization of a host/vector system for heterologous gene expression by Hansenula polymorpha. Curr Genet 19: 81–87

    Article  CAS  Google Scholar 

  • Sudbery PE, Gleeson MAG (1989) Genetic manipulation of methylotrophic yeasts. In: Walton EF, Yarranton GT (eds) Molecular and cell biology of yeasts. Blackie, Glasgow, pp 304–329

    Google Scholar 

  • Sulter GJ, Looyenga L, Veenhuis M, Harder M (1990a) Occurrence of peroxisomal membrane proteins in methylotrophic yeasts grown under different conditions. Yeast 6: 35–43

    Article  PubMed  CAS  Google Scholar 

  • Sulter GJ, Van der Klei IJ, Harder W, Veenhuis M (1990b) Expression and assembly of amine oxidase and D-amino acid oxidase in the cytoplasm of peroxisome-deficient mutants of the yeast Hansenula polymorpha during growth on primary amines or D-alanine as the sole nitrogen source. Yeast 6: 501–509

    Article  CAS  Google Scholar 

  • Sulter GJ, Van der Klei IJ, Schanstra JP, Harder W, Veenhuis M (1991) Ethanol metabolism in a peroxisome-deficient mutant of the yeast Hansenula polymorpha. FEMS Microbiol Lett 82: 297–302

    Article  CAS  Google Scholar 

  • Tarutina M, Tolstorukov II (1992) Mechanism of transformation of the methylotrophic yeast Pichia methanolica (P. pinus) MH4 with the LEU2 gene of the yeast Saccharomyces cerevisiae. Yeast 8: S622

    Google Scholar 

  • Tikhomirova LP, Ikonomova RN, Kuznetsova EN (1986) Evidence for autonomous replication and stabilization of recombinant plasmids in transformants of yeast Hansenula polymorpha. Curr Genet 10: 741–747

    Article  PubMed  CAS  Google Scholar 

  • Titorenko VI, Trotsenko YA (1983) Selection of mutants sythesizing branched-chain amino acids in the methylotrophic yeast Hansenula polymorpha. Microbiology 52: 774–780

    Google Scholar 

  • Titorenko VI, Khodurskii AB, Teslyar GE, Sibirny AA (1990) Identification of new genes controlling catabolite repression of the synthesis of alcohol oxidase and catalase in the methylotrophic yeast, Pichia pinus. Genetika 27: 625–635

    Google Scholar 

  • Tolstorukov II, Efremov BD (1984) Genetic mapping of yeast Pichia pinua. II. Mapping by tetrad analysis. Genetika 20: 1099–1107

    Google Scholar 

  • Tolstorukov II, Dutova TA, Benevolenskii SV, Soom YO (1977) Hybridization and genetic analysis of the methanol yeast Pichia pinus. Genetika 13: 322–329

    CAS  Google Scholar 

  • Tolstorukov II, Efremov BD, Bliznik KM (1983) Construction of a genetic map of the yeast Pichia pinus. I. Determination of linage groups using induced mitotic haploidization. Genetika 19: 897–902

    Google Scholar 

  • Tolstorukov II, Efremov BD, Benevolensky SV, Titorenko VI, Sibirni AA (1989) Mutants of the methylotrophic yeast Pichia pinus defective in C2 metabolism. Yeast 5: 179–186

    Article  CAS  Google Scholar 

  • Tschopp JF, Cregg JM (1991) Heterologous gene expression in methylotrophic yeasts. In: Goldberg I, Rokem JS (eds) Biology of methylotrophs. Butterworth-Heinemann, Boston, pp 305–322 (Biotechnology series, vol 18 )

    Google Scholar 

  • Tschopp JF, Brust PF, Cregg JM, Stillman CA, Gingeras TR (1987a) Expression of the lacZgene from two methanol-reguated promoters in Pichia pastoris. Nucleic Acids Res 15: 3859–3876

    Article  PubMed  CAS  Google Scholar 

  • Tschopp JF, Sverlov G, Kosson R, Craig W, Grinna L (1987b) High-level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris. Bio/Tchnology 5: 1305–1308

    Article  CAS  Google Scholar 

  • Tsukamoto T, Yokota S, Fujiki Y (1990) Isolation and characterization of Chinese hamster ovary cell mutants defective in assembly of peroxisomes. J Cell Biol 110: 651–660

    Article  PubMed  CAS  Google Scholar 

  • Van der Klei IJ, Harder W, Veenhuis M (1991a) Methanol metabolism in a peroxisome-deficient mutant of Hansenula polymorpha: a physiological study. Arch Microbiol 156: 15–23

    Article  PubMed  Google Scholar 

  • Van der Klei IJ, Harder W, Veenhuis M (1991b) Selective inactivation of alcohol oxidase in two peroxisome-deficient mutants of the yeast Hansenula polymorpha. Yeast 7: 813–821

    Article  PubMed  Google Scholar 

  • Van Dijken JP, Van der Bosch E, Hermanns JJ, Rodriguez de Miranda L, Scheffers WA (1986) Alcoholic fermentation by “non-fermentative” yeasts. Yeast 2: 123–127

    Article  PubMed  Google Scholar 

  • Veale RA, Guiseppin MLF, Van Eijk HM, Sudbery PE, Verrips CT (1992) Development of a strain of Hansenula polymorpha for the efficient expression of guar a-galactosidase. Yeast 8: 361–372

    Article  PubMed  CAS  Google Scholar 

  • Veenhuis M, Harder W (1991) Microbodies. In: Rose AH, Harrison JS (eds) The yeasts, vol 4, 2nd edn, Academic Press, London, pp 601–653

    Google Scholar 

  • Veenhuis M, Van Dijken JP, Harder W (1983) The significance of peroxisomes in the metabolism of one-carbon compunds in yeasts. In: Rose AH, Gareth Morris J, Tempest DW (eds) Advances in microbial physiology. Academic Press, London, pp 1–82

    Google Scholar 

  • Veenhuis M, Kram AM, Kunau WH, Harder W (1990) Excessive membrane development following exposure of the methylotrophic yeast Hansenula polymorpha to oleic acid-containing media. Yeast 6: 511–519

    Article  CAS  Google Scholar 

  • Veenhuis M, Van der Klei IJ, Titorenko VI, Harder W (1992) Hansenula polymorpha: an attractive model organism for molecular studies of peroxisome biogenesis and function. FEMS Microbiol Lett (in press)

    Google Scholar 

  • Verduyn C, Giuseppin MLF, Scheffers WA, Van Dijken JP (1988) Hydrogen peroxide metabolism in yeasts. Appl Environ Microbiol 54: 2086–2090

    PubMed  CAS  Google Scholar 

  • Verduyn C, Van Wijngaarden CJ, Scheffers WA, Van Dijken JP (1991) Hydrogen peroxide as an electron acceptor for mitochondrial respiration in the yeast Hansenula polymorpha. Yeast 7: 137–146

    Article  PubMed  CAS  Google Scholar 

  • Vonck J, Van Bruggen EFG (1992) Architecture of peroxisomal alcohol oxidase crystals from the methylotrophic yeast Hansenula polymorpha as deduced by electron microscopy. J Bacteriol 174: 5391–5399

    PubMed  CAS  Google Scholar 

  • Wegner EH (1983) US Patent 4,414,329. Nov 8, 1983

    Google Scholar 

  • Wegner GH (1990) Emerging applications of methylotrophic yeasts. FEMS Microbial Rev 87:279–284 Wegner EH, Harder W ( 1986 ) Methylotrophic yeasts. In: Van Versefeld HW, Duine JA (eds) Microbial

    Google Scholar 

  • Growth on CI Compounds: Proceedings of the 5th International Symposium. Martinus Nijhoff, Dordrecht, pp 131–138

    Google Scholar 

  • Wiebel FF, Kunau WH (1992) The Past protein essential for peroxisome biogenesis is related to ubiquitin-conjugating enzymes. Nature 359: 73–76

    Article  PubMed  CAS  Google Scholar 

  • Zoeller RA, Raetz CRH (1986) Isolation of animal cell mutants deficient in plasmalogen biosynthesis and peroxisomal assembly. Proc Natl Acad Sci USA 83: 5170–5174

    Article  PubMed  CAS  Google Scholar 

  • Zoeller RA, Allen LAH, Santos MJ, Lazarow PB, Hashimoto T, Tartakoff AM, Raetz CRH (1989) Chinese hamster ovary cell mutants defective in peroxisome biogenesis. J Biol Chem 264: 21872–21878

    PubMed  CAS  Google Scholar 

  • Zoroddu MA, Bonomo RP, Di Bilio AJ, Berardi E, Meloni MGL (1991) EPR study on vanadyl and vanadate ion retention by a thermotolerant yeast. J Inorg Biochem 43: 731–738

    Article  CAS  Google Scholar 

  • Zwart K, Veenhuis M, Van Dijken JP, Harder W (1980) Development of amine oxidase-containing peroxisomes in yeasts during growth on glucose in the presence of methyamine as the sole source of nitrogen. Arch Microbiol 126: 117–126

    Article  PubMed  CAS  Google Scholar 

  • Zwart K, Veenhuis M, Plat G, Harder W (1983) Characterization of glyoxysomes in yeasts and their transformation into peroxisomes in response to changes in environmental conditions. Arch Microbiol 136: 28–38

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  1. E. Berardi
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  1. Avenida Belgrano y Pasaje Caseros, PROIMI, 4000, S.M. de Tucumán, Argentina

    John F. T. Spencer  & Dorothy M. Spencer  & 

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© 1997 Springer-Verlag Berlin Heidelberg

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Berardi, E. (1997). Genetics and Molecular Biology of Methylotrophic Yeasts. In: Spencer, J.F.T., Spencer, D.M. (eds) Yeasts in Natural and Artificial Habitats. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03370-8_15

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  • DOI: https://doi.org/10.1007/978-3-662-03370-8_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08160-6

  • Online ISBN: 978-3-662-03370-8

  • eBook Packages: Springer Book Archive

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