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Transformation of the methylotrophic yeast Hansenula polymorpha by autonomous replication and integration vectors

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Summary

A high frequency transformation system for the methylotrophic yeast Hansenula polymorpha has been developed. This system depends on complementation of isolated uracil auxotrophs by the URA3 gene of Saccharomyces cerevisiae. Maintenance of the uracil prototrophy is based on integration of plasmid YIp5 at random sites within the H. polymorpha genome and on autonomously replicating plasmids containing ARS1 of S. cerevisiae or related sequences cloned from the host DNA. The sequence of one autonomously replicating sequence (HARS1) from H. polymorpha has been determined showing an AT-rich region of 9 bp with some similarity to the consensus sequence of known eukaryotic replication origins. Mitotic loss of autonomously replicating sequences is high; selection for stable uracil prototrophs yields multiple tandem arrangement of the transformed DNA with no detectable loss of the phenotype on non-selective medium. These features offer the possibility for extensive gene expression in H. polymorpha.

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Authors and Affiliations

  1. Institut für Mikrobiologie, Universität Düsseldorf, Universitätsstrasse 1, D-4000, Düsseldorf, Federal Republic of Germany

    Rainer Roggenkamp, Hans Hansen, Michael Eckart, Zbigniew Janowicz & Cornelis P. Hollenberg

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  1. Rainer Roggenkamp
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  2. Hans Hansen
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  3. Michael Eckart
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  4. Zbigniew Janowicz
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  5. Cornelis P. Hollenberg
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Communicated by R. Herrmann

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Roggenkamp, R., Hansen, H., Eckart, M. et al. Transformation of the methylotrophic yeast Hansenula polymorpha by autonomous replication and integration vectors. Mol Gen Genet 202, 302–308 (1986). https://doi.org/10.1007/BF00331655

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  • DOI: https://doi.org/10.1007/BF00331655

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