Summary
The polymerase chain reaction (PCR) amplification technique of viral ribonucleic acids has been evaluated as an alternative protocol for the diagnosis, typing, and molecular epidemiology of the rabies virus. Convenient regions of the viral genome have been delineated for each purpose. In its current form, PCR presents the same sensitivity but is slower than the routine diagnosis techniques by immunodetection of viral nucleocapsid. It can be already considered as a suitable confirmatory method for diagnosis and promises to be more competitive after further improvement. On the other hand, it advantageously compares with monoclonal antibody analysis for typing by restriction fragment length polymorphism. Limited panels of restriction enzyme can be defined to distinguish the vaccinal strains, as well as wild viral isolates of different geographic provenance. Finally, PCR suffers no concurrent technique in the ultimate identification of the viral isolate by direct sequence determination of the amplified segment. This allows us to undertake a worldwide molecular epidemiological study of rabies, and to reinvestigate the taxonomy of the Lyssavirus genus.
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© 1992 Springer-Verlag Berlin Heidelberg
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Tordo, N., Bourhy, H., Sacramento, D. (1992). Polymerase Chain Reaction Technology for Rabies Virus. In: Becker, Y., Darai, G. (eds) Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Frontiers of Virology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84766-0_29
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DOI: https://doi.org/10.1007/978-3-642-84766-0_29
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