Abstract
Most of eukaryotic microorganisms which are of industrial and clinical interest cannot be investigated by classical genetic analysis. Only the way we can promote genetic study with these microorganisms is the so-called reverse genetics by using recombinant DNA technology. The process of this approach consists of i) a finding of a characteristic and interesting property (P) in a certain microorganism (M), ii) isolation and structural analysis of a gene (G) related to (P), iii) construction of a host-vector system (H-V) and gene disruption system(G-D) for(M), iv) analysis of phenotype of a genetically engineered strain of (M) with a multicopied (G) or a disrupted (G) and v) providing direct evidence that (G) has a specific function to determine (P) in (M).
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© 1993 Springer-Verlag Berlin Heidelberg
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Takagi, M. (1993). Reverse genetics in a non-conventional yeast, Candida maltosa . In: Maresca, B., Kobayashi, G.S., Yamaguchi, H. (eds) Molecular Biology and its Application to Medical Mycology. NATO ASI Series, vol 69. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84625-0_3
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DOI: https://doi.org/10.1007/978-3-642-84625-0_3
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