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Cryoultramicrotomy for Autoradiography and Enzyme Cytochemistry

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Cryotechniques in Biological Electron Microscopy

Abstract

The introduction of cryoultramicrotomy as a new tool in histochemistry and cytochemistry has led to great expectations for autoradiography and enzyme cytochemistry, the classical methods in histochemistry and cytochemistry. It is an unpleasant task to summarize the present situation and to arrive at the conclusion that these promises are only partly fulfilled. For the autoradiography of diffusible substances, there seem to be only practical limitations (work and time), however, for enzyme cytochemistry, there are also theoretical limitations to the use of thin cryosections. Autoradiography and enzyme cytochemistry have thus hardly benefitted from the development of cryoultramicrotomy and this remains in sharp contrast to immunocytochemistry where the thin cryosection has found many applications and has become a sort of “(irnrmmo)-golden” standard (see also Boonstra et al., Chap. 11, this Vol.).

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Authors and Affiliations

  1. Department of Pathology/EM Unit, University of Maastricht, P.O.Box 616, N-6200 MD, Maastricht, The Netherlands

    Peter M. Frederik

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  1. Peter M. Frederik
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Editors and Affiliations

  1. Max-Planck-Institut für Verhaltensphysiologie, D-8131, Seewiesen, Germany

    Rudolf Alexander Steinbrecht

  2. Max-Planck-Institut für Systemphysiologie, Rheinlanddamm 201, D-4600, Dortmund, Germany

    Karl Zierold

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© 1987 Springer-Verlag Berlin Heidelberg

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Frederik, P.M. (1987). Cryoultramicrotomy for Autoradiography and Enzyme Cytochemistry. In: Steinbrecht, R.A., Zierold, K. (eds) Cryotechniques in Biological Electron Microscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72815-0_12

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  • DOI: https://doi.org/10.1007/978-3-642-72815-0_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72817-4

  • Online ISBN: 978-3-642-72815-0

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