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A new cerium-based method for cytochemical localization of thiamine pyrophosphatase in the Golgi complex of rat hepatocytes

Comparison with the lead technique

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Summary

The use of cerium chloride for the localization of thiamine-pyrophosphatase (TPPase) in rat liver parenchymal cells has been investigated and the results are compared with the classical lead capture method. A medium containing 3 mM cerium chloride gave the most uniform and consistent results with a homogenous electron dense reaction product in the first trans lamella of the Golgi complex and a weak staining of endoplasmic reticulum. The fine deposits of cerium phosphate filled completely the first trans Golgi cisterna. In contrast the reaction product of the lead-based method appeared clumpy and aggregated with an irregular distribution over both Golgi complex and endoplasmic reticulum. Higher and lower concentrations of cerium chloride than 3 mM gave inconsistent results. The present study demonstrates that the cerium-based method is superior to the classical lead-technique for the localization of TPPase.

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References

  • Arnold G, Liscum L, Holtzman E (1979) Ultrastructural localization of D-amino acid oxidase in microperoxisomes of rat nervous system. J Histochem Cytochem 27:735–745

    Google Scholar 

  • Blok J, Onderwater JJM, de Water R, Ginsel LA (1982) A cytochemical method for the demonstration of 5′nucleotidase in mouse peritoneal macrophages, with cerium ions used as trapping agent. Histochemistry 75:437–443

    Google Scholar 

  • Briggs RT, Draht DB, Karnovsky ML, Karnovsky MJ (1975) Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method. J Cell Biol 67:566–586

    Google Scholar 

  • Cheetham RD, Morré DJ, Pannek C, Friend DS (1971) Isolation of a Golgi apparatus-rich fraction from rat liver. J Cell Biol 49:899–905

    Google Scholar 

  • Christie KN, Stoward PJ (1982) The cytochemical reactivity of cerium ions with cardiac muscles. Acta Histochem Cytochem 15:656–672

    Google Scholar 

  • Farquhar MG, Bergeron JJM, Palade GE (1974) Cytochemistry of Golgi fractions prepared from rat liver. J Cell Biol 60:8–25

    Google Scholar 

  • Farquhar MG, Palade GE (1981) The Golgi apparatus (complex)-(1954–1981)-from artifact to center stage. J Cell Biol 91:77s-103s

    Google Scholar 

  • Fujimoto T, Inomata K, Ogawa K (1982) A cerium method for the ultracystochemical localization of monoamine oxidase activity. Histochem J 14:87–98

    Google Scholar 

  • Goldfischer S, Essner E, Schiller B (1971) Nucleoside diphosphatase and thiamine pyrophosphatase activities in the endoplasmic reticulum and golgi apparatus. J Histochem Cytochem 19:349–360

    Google Scholar 

  • Hulstaert CD, Kalicharan D, Hardonk MJ (1983) Cytochemical demonstration of phosphatases in rat liver by a cerium-based method in combination with osmium tetroxide and potassium ferrocyanide postfixation. Histochemistry 78:71–79

    Google Scholar 

  • Novikoff AB, Goldfischer S (1961) Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytochemical studies. Proc Natl Acad Sci USA 47:802–810

    Google Scholar 

  • Oliver C, Hand AR (1983) Enzyme modulation of the Golgi apparatus and GERL: a cytochemical study of parotid acinar cells. J Histochem Cytochem 31:1041–1048

    Google Scholar 

  • Robinson JM, Briggs RT, Karnovsky MJ (1978) Localization of D-amino acid oxidase on the cell surface of human polymorphonuclear leukocytes. J Cell Biol 77:59–71

    Google Scholar 

  • Robinson JM, Karnovsky MJ (1982) Cytochemical localization of the ectoenzyme 5′-nucleotidase by a new cytochemical method. J Histochem Cytochem 30:507 (Abstract)

    Google Scholar 

  • Veenhuis M, Wendelaar Bonga SE (1979) Cytochemical localization of catalase and several hydrogen peroxide-producing oxidases in the nucleoids and matrix of rat liver peroxisomes. Histochem J 11:561–572

    Google Scholar 

  • Veenhuis M, Van Dijken JP, Harder W (1980a) A new method for the cytochemical demonstration of phosphatase activities in yeasts based on the use of cerous ions. FEMS Microbiol Lett 9:285–291

    Google Scholar 

  • Veenhuis M, Flik G, Wendelaar Bonga SE (1980b) CeCl3: a new capturing agent for the cytochemical demonstration of phosphatase activity. In: Brederoo P, De Priester W (eds) Electron microscopy 1980, vol 2. Seventh European Congress on Electron Microscopy Foundation, Leiden, pp 284–285

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, II. Division, University of Heidelberg, Im Neuenheimer Feld 307, D-6900, Heidelberg, Federal Republic of Germany

    S. Angermüller & H. Fahimi

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  1. S. Angermüller
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  2. H. Fahimi
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Angermüller, S., Fahimi, H. A new cerium-based method for cytochemical localization of thiamine pyrophosphatase in the Golgi complex of rat hepatocytes. Histochemistry 80, 107–111 (1984). https://doi.org/10.1007/BF00679983

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  • DOI: https://doi.org/10.1007/BF00679983

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