Abstract
Since the first report by Cocking (1960), protoplasts have become an important experimental tool for plant propagation as well as for physiological and biochemical studies. Plant protoplasts are excellent sources for the isolation of functional organelles such as chloroplasts, mitochondria, or vacuoles, and have been the basis for a wide range of investigations into subcellular compartmentation of metabolic pathways, or transport properties of distinct membranes (Hampp 1980, 1985). With regards to fungi there is considerably less information available (Peberdy 1989).
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© 1998 Springer-Verlag Berlin Heidelberg
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Hampp, R., Stülten, C., Nehls, U. (1998). Isolation and Regeneration of Protoplasts from Ectomycorrhizal Fungi. In: Varma, A. (eds) Mycorrhiza Manual. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60268-9_8
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DOI: https://doi.org/10.1007/978-3-642-60268-9_8
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