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Highly Productive Plant Continuous Cell-Free System

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Cell-Free Translation Systems

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Abstract

The development of a system capable of synthesizing any desired protein on a preparative scale is one of the most important endeavors in biotechnology today. Three strategies are currently being used: chemical synthesis, in vivo expression, and cell-free protein synthesis. The first two methods have severe limitations: chemical synthesis is not feasible for the synthesis of long peptides because of low yield, and in vivo expression can produce only those proteins that do not affect the physiology of the host cell [1–3]. Cell-free translation systems, in contrast, can synthesize proteins with high speed and accuracy, approaching in vivo rates [4–5], and they can express proteins that would interfere with cell physiology. However, they are relatively inefficient because of their instability[6].

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Authors
  1. Kairat Madin
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  2. Tatsuya Sawasaki
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  3. Yaeta Endo
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Editor information

Editors and Affiliations

  1. Institute for Protein Research, Russian Academy of Sciences, 142292, Pushchino, Moscow Region, Russia

    Alexander S. Spirin

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© 2002 Springer-Verlag Berlin Heidelberg

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Madin, K., Sawasaki, T., Endo, Y. (2002). Highly Productive Plant Continuous Cell-Free System. In: Spirin, A.S. (eds) Cell-Free Translation Systems. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59379-6_9

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  • DOI: https://doi.org/10.1007/978-3-642-59379-6_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63956-2

  • Online ISBN: 978-3-642-59379-6

  • eBook Packages: Springer Book Archive

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