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Cell-Free Translation Systems

  • Book
  • © 2002

Overview

Editors:
  1. Alexander S. Spirin

    1. Institute for Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia

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Table of contents (24 chapters)

  1. Front Matter

    Pages I-VIII
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Cell-Free Protein Synthesis

      • Alexander S. Spirin
      Pages 3-20

  3. Improved Classical (Batch) Cell-Free Translation Systems

    1. Front Matter

      Pages 21-21
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    2. Improved Batch Translation System Based on E. coli Extract

      • Thorsten Lamla, Volker A. Erdmann
      Pages 23-39

    3. Improved Composition and Energy Supply for Bacterial Batch Systems

      • Dong-Myung Kim, James R. Swartz
      Pages 41-52

    4. PURE System

      • Takuya Ueda, Akio Inoue, Midori Kaida, Ryoko Baba, Yoshihiro Shimizu
      Pages 53-60

    5. Direct Expression of PCR Products in Cell-Free Translation Systems

      • Anatoly T. Gudkov, Kirill A. Martemyanov
      Pages 61-66

    6. Yeast Cell-Free Translation Systems

      • Michael Altmann, Hans Trachsel
      Pages 67-78

    7. Poly(A)-Dependent Cell-Free Translation Systems from Animal Cells

      • Giovanna Bergamini, Fátima Gebauer
      Pages 79-88

  4. Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems

    1. Front Matter

      Pages 89-89
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    2. Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems and Reactors

      • Vladimir A. Shirokov, Peter N. Simonenko, Sergey V. Biryukov, Alexander S. Spirin
      Pages 91-107

    3. Highly Productive Plant Continuous Cell-Free System

      • Kairat Madin, Tatsuya Sawasaki, Yaeta Endo
      Pages 109-119

    4. Matrix Reactor: A New Scalable Reactor Principle for Cell-Free Protein Expression

      • Bernd Buchberger, Wolfgang Mutter, Albert Röder
      Pages 121-128

  5. Protein Folding in Cell-Free Translation System

    1. Front Matter

      Pages 129-129
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    2. Co-Translational Protein Folding in Prokaryotic and Eukaryotic Cell-Free Translation Systems

      • Vyacheslav A. Kolb, Aigar Kommer, Alexander S. Spirin
      Pages 131-140

    3. Expression of an Aggregation-Prone Protein in the RTS 500 System

      • Jean-Michel Betton
      Pages 141-147

    4. Expression of Soluble Murine Endostatin with the RTS System

      • Stacey Traviglia, Monique van Hoek
      Pages 149-156

    5. Cell-Free Expression of Soluble Human Erythropoietin

      • Cordula Nemetz, Stephanie Wessner, Simone Krupka, Manfred Watzele, Wolfgang Mutter
      Pages 157-163

    6. Cell-Free Expression of a 127 kDa Protein: The Catalytic Subunit of Human Telomerase

      • Robin Steigerwald, Cordula Nemetz, Bärbel Walckhoff, Thomas Emrich
      Pages 165-173
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Keywords

About this book

This is a unique book that describes the most recent achievements in the methodology of protein biosynthesis under cell-free conditions. Various versions of cell-free protein-synthesizing systems and their applications to production of individual proteins on a preparative scale are reviewed. The most recent, advanced methodologies, such as continuous-exchange and continuous-flow cell-free systems and novel effecting batch-format cell-free procedures, are considered. Special attention is drawn to the possibilities of structural (NMR; X-ray) analysis of various gene expression products with the use of a new generation of cell-free systems.

Editors and Affiliations

  • Institute for Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia

    Alexander S. Spirin

Bibliographic Information

  • Book Title: Cell-Free Translation Systems

  • Editors: Alexander S. Spirin

  • DOI: https://doi.org/10.1007/978-3-642-59379-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2002

  • Softcover ISBN: 978-3-642-63956-2Published: 09 April 2012

  • eBook ISBN: 978-3-642-59379-6Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: VIII, 259

  • Number of Illustrations: 74 b/w illustrations

  • Topics: Biotechnology

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