Abstract
The advent of fluorescence in situ hybridization (FISH) rendered it possible to obtain “cytogenetic information” from interphase nuclei (Langer et aL, 1981, Pinkel et al, 1986). Karyotypic analysis by direct demonstration of DNA sequences in interphase nuclei (interphase cytogenetics or interphase FISH) can be applied to a wide variety of cellular material, including cytogenetic preparations, fresh material (touch-preparations), cryofixed and paraffin-embedded tissue. In contrary to metaphase FISH, only cosmids, PI-clones, BACs, YACs and satellite probes are suitable for interphase FISH. Detection and quantitation of structural chromosome aberrations, of microdeletions or microduplications and oncogene amplification or deletion of tumor suppressor genes in solid tumors thus became possible.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Dhingra, K, Sahin, A, Supak, J, Kim, SY, Hortobagyi, G, Hittelman, WN (1992) Chromosome in situ hybridization on formalin-fixed mammary tissue using non-isoto-pic, non-fluorescent probes: technical considerations and biological implications. Breast Cancer Res Treat, 23:201–210
Hedley, DW, Friedlander, ML, Taylor, IW, Rugg, CA, Musgrove, EA (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem, 31:1333–1335
Langer, PR, Waldrop, AA, Ward, DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes. Proc Natl Acad Sci USA, 78:6633–6637
Liehr, T, Stübinger, A, Thoma, K, Tulusan, HA, Gebhart, E (1994) Comparative interphase cytogenetics using FISH on human ovarian carcinomas. Anticancer Res, 14:183–188
Liehr, T, Thoma, K, Kammler, K, Gehring, C, Ekici, A, Bathke, KD, Grehl, H, Rautenstrauss, B (1995) Direct preparation of uncultured EDTA-treated or heparinized blood for interphase FISH analysis. Appl Cytogenet, 21:185–188
Liehr,T, Grehl, H, Rautenstrauss, B (1996) A rapid method for FISH analysis on interphase nuclei extracted from cryofixed tissue. Trends in Genetics, 12: 505–506
Liehr, T, Grehl, H, Rautenstrauss, B (1997) Molecular diagnosis of PMP22-associated neuropathies using fluorescence in situ hybridization (FISH) on archival peripheral nerve tissue preparations. Acta Neuropathol, 94:266–271
Long, AA, Komminoth, P, Lee, E, Wolfe, HJ (1993) Comparison of indirect and direct in-situ polymerase chain reaction in cell preparations and tissue sections. Detection of viral DNA, gene rearrangements and chromosomal translocations. Histochem, 99:151–162
Neubauer, S, Liehr, T, Tulusan, HA, Gebhart, E (1994) Interphase cytogenetics by FISH on archival paraffin material and cultured cells of human ovarian tumors. Int J Oncol, 4:317–321
Pinkel, D, Straume, T, Gray, JW (1986) Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA, 83:2934–2938
Zang, KD, Singer H (1967) Chromosomal constitution of meningiomas. Nature, 216:84–85
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Rautenstrauss, B., Liehr, T. (2002). Nucleus Extraction from Cryofixed Tissue. In: Rautenstrauss, B.W., Liehr, T. (eds) FISH Technology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56404-8_14
Download citation
DOI: https://doi.org/10.1007/978-3-642-56404-8_14
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47739-3
Online ISBN: 978-3-642-56404-8
eBook Packages: Springer Book Archive