FISH Technology

Editors:

ISBN: 978-3-642-47739-3 (Print) 978-3-642-56404-8 (Online)

Table of contents (36 chapters)

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  1. Front Matter

    Pages I-X

  2. The Standard FISH Procedure — an Introduction

    1. Front Matter

      Pages 1-1

    2. No Access

      Chapter

      Pages 3-50

      FISH Techniques, FISH Probes and Their Applications in Medicine and Biology — An Overview

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      Chapter

      Pages 51-69

      Microscopy and Imaging Systems

  3. FISH on Native, Human Tissues

    1. Front Matter

      Pages 71-71

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      Chapter

      Pages 73-81

      FISH on Chromosome Preparations of Peripheral Blood

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      Chapter

      Pages 82-89

      FISH on Uncultured Blood or Bone Marrow

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      Chapter

      Pages 90-96

      FISH on Blood or Bone Marrow Smears

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      Chapter

      Pages 97-108

      Epithelial Cells from Buccal Smears and Urine

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      Chapter

      Pages 109-114

      FISH on Hair Root Cells

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      Chapter

      Pages 115-126

      Amniotic Fluid Cells — Uncultured

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      Chapter

      Pages 127-137

      Human Sperm Cells

  4. FISH on Archival, Human Tissues

    1. Front Matter

      Pages 139-139

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      Chapter

      Pages 141-147

      Archival Cytogenetic Slides

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      Chapter

      Pages 148-161

      Formalin-Fixed and Paraffin-Embedded Tissue Sections

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      Chapter

      Pages 162-170

      Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue

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      Chapter

      Pages 171-175

      Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue

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      Chapter

      Pages 176-184

      Nucleus Extraction from Cryofixed Tissue

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      Chapter

      Pages 185-196

      Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells

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      Chapter

      Pages 197-217

      Comparative Genomic Hybridization

  5. Special FISH Techniques (Fiber-FlSH, PNA-FlSH, Co-FISH, NU-FISH)

    1. Front Matter

      Pages 219-219

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      Chapter

      Pages 221-225

      High Resolution FISH of Stretched Chromosome Fibers

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      Chapter

      Pages 226-253

      Quantitative DNA Fiber Mapping

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      Chapter

      Pages 254-261

      PNA-FISH

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      Chapter

      Pages 262-271

      Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH

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