Abstract
The submerged fermentation medium was optimized in order to improve the keratinase activity produced by Bacillus licheniformis TCCC 11593. The keratinase showed maximum activity 98.0 U/mL after 48 h fermentation in 50 °C when adding 1 g/L maltose and 1.5 g/L NH4Cl as the supplement of carbon and nitrogen sources. The optimal feather quantity was 20 g/L in fermentation. Ca2+ and Mn2+ inhibited the keratinase activity in fermentation medium, while keratinase activity was improved by adding 0.3 g/L Mg2+ to fermentation medium. At the same time, it was found that the complement of alkaline protease and neutral protease further improved the feather degradation in fermentation process. The total amino acid yield can reach 167.43 mg/g.
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Acknowledgments
The authors gratefully acknowledge the support of Tianjin Municipal Science and Technology Committee (10ZCKFNC01700) and the National High Technology Research and Development Program (‘863’ Plan) (2013AA102106 and 2012AA021502).
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Li, Y., Fan, S., Chen, S., Er, H., Du, J., Lu, F. (2014). Study on the Fermentation Conditions and the Application in Feather Degradation of Keratinase Produced by Bacillus licheniformis . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_10
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DOI: https://doi.org/10.1007/978-3-642-37916-1_10
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