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Molecular Tools, Expression Profiling

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The Impact of Tumor Biology on Cancer Treatment and Multidisciplinary Strategies

Part of the book series: Medical Radiology ((Med Radiol Radiat Oncol))

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Abstract

High-throughput technologies of modern biology provide “molecular portraits” of tissues and have entered the field of oncology. In the present chapter, we describe tools of high-throughput expression analysis in transcriptomics and proteomics, with an emphasis on microarrays, two-dimensional electrophoresis, and mass spectrometry. Options and limitations in data production, extraction, and interpretation are outlined. Problems of sensitivity, specificity, multiple testing, and noise are discussed. As a concrete example, we review the application of these tools to the field of breast cancer, where expression analyses already contribute to individual treatment decisions.

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Author information

Authors and Affiliations

  1. Klinik für Pädiatrie m. S. Neurologie, Charité – Universitätsmedizin Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353, Berlin, Germany

    Angela M. Kaindl MD

  2. Laboratoire de Neurologie du Développement UMR 676 Inserm-Paris 7 & Service de Neuropédiatrie, Hôpital Robert Debré, 48 Blvd. Serurier, 75019, Paris, France

    Angela M. Kaindl MD

  3. Institut für Humangenetik, Klinikum Rechts der Isar der Technischen Universität München, Trogerstraße 32, 81675, München, Germany

    Konrad Oexle MD

Authors
  1. Angela M. Kaindl MD
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  2. Konrad Oexle MD
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Editor information

Editors and Affiliations

  1. Klinik und Poliklinik für Strahlentherapie und Radiologische Onkologie, Klinikum Rechts der Isar der Technischen Universität München, Ismaninger Straße 22, 81675, München, Germany

    Michael Molls MD

  2. Institute of Physiology and Pathophysiology, University Medical Center, Duesbergweg 6, 55099, Mainz, Germany

    Peter Vaupel MD, MA

  3. Department of Internal Medicine – Oncology, Nordlandssykehuset HF Hospital, 8092, Bodø, Norway

    Carsten Nieder MD

  4. Department of Radiation Oncology, Virginia Commonwealth University School of Medicine, 401 College Street, 980058, 23298-0058, Richmond, VA, USA

    Mitchell S. Anscher MD, FACR, FACRO

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© 2009 Springer-Verlag Berlin Heidelberg

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Kaindl, A., Oexle, K. (2009). Molecular Tools, Expression Profiling. In: Molls, M., Vaupel, P., Nieder, C., Anscher, M. (eds) The Impact of Tumor Biology on Cancer Treatment and Multidisciplinary Strategies. Medical Radiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74386-6_17

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  • DOI: https://doi.org/10.1007/978-3-540-74386-6_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-74385-9

  • Online ISBN: 978-3-540-74386-6

  • eBook Packages: MedicineMedicine (R0)

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