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Raman Imaging of Biomedical Samples

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Confocal Raman Microscopy

Part of the book series: Springer Series in Surface Sciences ((SSSUR,volume 66))

Abstract

Fluorescence microscopy, a gold standard in the tissue or cell imaging, has several drawbacks, with a severe one being its disability to elucidate the chemical characteristics of the sample. Confocal Raman microscopy is a tempting label free alternative to fluorescence and a prospective future method of medical diagnostics. Raman measurements of tissues can provide information about their chemical composition, i.e. main components but also specific compounds, and their changes upon the development of patholog or its treatment. The analysis is based on characteristic marker bands, provided they can be identified in the spectra, or by more common approach using chemometrics. Moreover, Raman microscopy can be used to detect small biochemical alterations and their respective distribution in a single cell or even at sub-cellular level. The impact of various factors, including the uptake of drugs, bioactive compounds or non-chemical stressors can be observed using such investigations of cells.

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Notes

  1. 1.

    All procedures involving animals were conducted in accordance with the Guidelines for Animal Care and Treatment of the European Union and were approved by the 1st Local Animal Ethics Commission in Krakow (decision no. 51/2009).

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Acknowledgements

The financial support by National Science Centre (DEC-2013/08/A/ST4/00308) and by the European Union under the European Regional Development Fund (grant coordinated by JCET-UJ, POIG.01.01.02-00-069/09) is acknowledged.

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Authors and Affiliations

  1. Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland

    Agnieszka Kaczor, Katarzyna Majzner, Kamila Kochan, Marta Z. Pacia & Malgorzata Baranska

  2. Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland

    Agnieszka Kaczor, Katarzyna M. Marzec, Katarzyna Majzner, Kamila Kochan, Marta Z. Pacia & Malgorzata Baranska

  3. Center for Medical Genomics (OMICRON), Jagiellonian University Medical College, Kopernika 7C, 31-034, Krakow, Poland

    Katarzyna M. Marzec

Authors
  1. Agnieszka Kaczor
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  2. Katarzyna M. Marzec
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  3. Katarzyna Majzner
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  4. Kamila Kochan
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Correspondence to Malgorzata Baranska .

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Editors and Affiliations

  1. Wissenschaftliche Instrumente und Technologie GmbH, Ulm, Germany

    Jan Toporski

  2. Ulm, Germany

    Thomas Dieing

  3. Wissenschaftliche Instrumente und Technologie GmbH, Ulm, Germany

    Olaf Hollricher

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Kaczor, A., Marzec, K.M., Majzner, K., Kochan, K., Pacia, M.Z., Baranska, M. (2018). Raman Imaging of Biomedical Samples. In: Toporski, J., Dieing, T., Hollricher, O. (eds) Confocal Raman Microscopy. Springer Series in Surface Sciences, vol 66. Springer, Cham. https://doi.org/10.1007/978-3-319-75380-5_14

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