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Raman Microscopy : A Versatile Approach to Bio-Imaging

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Raman Imaging

Part of the book series: Springer Series in Optical Sciences ((SSOS,volume 168))

Abstract

Raman microscopy has become established as a key probe technique in biology and biomedicine. In combination with imaging and mapping it has been employed in the investigation of a diverse array of problems ranging from ex vivo and in vivo single cell studies to elucidation of the often complex, interacting structures which constitute human and animal tissues. This chapter emphasises the unique attributes of Raman microscopy as a bioimaging technique, including its non-invasive, spectral multiplexing ability, allied with high spatial resolution and underpinned by a range of multivariate data processing methods. A number of illustrative examples have been selected for discussion from the fields of molecular biology, ophthalmology, respiratory medicine as well as some non-medical examples. Recent advances and pointers to future activity in the uses of Raman microscopy as a structurally and functionally informative bioimaging technique are briefly considered.

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Notes

  1. 1.

    though there are fortunate exceptions [45] where, even with near-UV or visible excitation of biological samples, interference in the Raman scattering due to fluorescence does not arise.

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Acknowledgments

The authors wish to gratefully acknowledge the many contributions from collaborators and colleagues to the QUB-based studies included in this chapter. Vital financial support has been provided over several years from the UK Biotechnology and Biological Sciences Research Council, the Medical Research Council, Research and Development Office of NI Health Service and the Leverhulme Trust.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Queen’s University, Belfast, UK

    J. J. McGarvey

  2. Centre for Vision and Vascular Sciences School of Medicine, Dentistry and Biomedical Sciences, Queen’s University of Belfast, Belfast, BT7 1NN, UK

    J. Renwick Beattie

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Correspondence to J. J. McGarvey .

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    Arnaud Zoubir

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McGarvey, J.J., Renwick Beattie, J. (2012). Raman Microscopy : A Versatile Approach to Bio-Imaging. In: Zoubir, A. (eds) Raman Imaging. Springer Series in Optical Sciences, vol 168. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28252-2_7

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