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Acyl-Carbon Bond Cleaving Cytochrome P450 Enzymes: CYP17A1, CYP19A1 and CYP51A1

  • Chapter
Monooxygenase, Peroxidase and Peroxygenase Properties and Mechanisms of Cytochrome P450

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 851))

Abstract

Cytochrome P450 (P450 or CYP) enzymes in their resting state contain the heme-iron in a high-spin FeIII state. Binding of a substrate to a P450 enzyme allows transfer of the first electron, producing a FeII species that reacts with oxygen to generate a low-spin iron superoxide intermediate (FeIII–O–O•) ready to accept the second electron to produce an iron peroxy anion intermediate (a, FeIII–O–O−). In classical monooxygenation reactions, the peroxy anion upon protonation fragments to form the reactive Compound I intermediate (Por•+FeIV=O), or its ferryl radical resonance form (FeIV–O•). However, when the substrate projects a carbonyl functionality, of the type b, at the active site as is the case for reactions catalyzed by CYP17A1, CYP19A1 and CYP51A1, the peroxy anion (FeIII–O–O−) is trapped, yielding a tetrahedral intermediate (c) that fragments to an acyl-carbon cleavage product (d plus an acid). Analogous acyl-carbon cleavage reactions are also catalyzed by certain hepatic P450s and CYP125A1 from Mycobacterium tuberculosis. A further improvisation on the theme is provided by aldehyde deformylases that convert long-chain aliphatic aldehydes to hydrocarbons. CYP17A1 is involved in the biosynthesis of corticoids as well as androgens. The flux toward these two classes of hormones seems to be regulated by cytochrome b 5, at the level of the acyl-carbon cleavage reaction. It is this regulation of CYP17A1 that provides a safety mechanism, ensuring that during corticoid biosynthesis, which requires 17α-hydroxylation by CYP17A1, androgen formation is avoided (Fig. 4.1).

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Reviews

Apart from citations in the text [2, 5–8, 75], useful reviews dealing with specific aspects of the subject are available [80, 81].

Note added in proof

Recently another alternative to the mechanism of Scheme 4.11 has been suggested (Yoshimoto FK, Guengerich FP, J Am Chem Soc 136: 15016–25).

Acknowledgments

We acknowledge with pleasure the help of Dr. Peter Lee-Robichaud in writing the section on the interaction of CYP17A1 with cytochrome b 5, an area in which he has made seminal contributions.

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Authors and Affiliations

  1. School of Biological Sciences, University of the Punjab, New Campus, Lahore, 54590, Pakistan

    Muhammad Akhtar

  2. Centre for Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK

    Muhammad Akhtar & J. Neville Wright

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  1. Muhammad Akhtar
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  2. J. Neville Wright
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Correspondence to Muhammad Akhtar .

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Editors and Affiliations

  1. Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, British Columbia, Canada

    Eugene G. Hrycay

  2. Fac. of Pharmaceutical Sciences, The University of British Columbia , Vancouver, British Columbia, Canada

    Stelvio M. Bandiera

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Akhtar, M., Wright, J.N. (2015). Acyl-Carbon Bond Cleaving Cytochrome P450 Enzymes: CYP17A1, CYP19A1 and CYP51A1. In: Hrycay, E., Bandiera, S. (eds) Monooxygenase, Peroxidase and Peroxygenase Properties and Mechanisms of Cytochrome P450. Advances in Experimental Medicine and Biology, vol 851. Springer, Cham. https://doi.org/10.1007/978-3-319-16009-2_4

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