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Epigenetic Reprogramming with Oocyte Molecules

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Nuclear Reprogramming and Stem Cells

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

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Abstract

Following the demonstration that somatic cells can be fully reprogrammed to totipotency after transfer into enucleated eggs, the prospect of developing methods of cellular reprogramming for autologous cell therapies has come a little closer. How the egg reprogrammes somatic cells is little understood, and much work has been devoted towards elucidating the basis of these reprogramming events. Here, we have reviewed the current knowledge of reprogramming after nuclear transplantation and we present an experimental approach using the axolotl oocyte as a tool to broaden our understanding of the basic mechanisms of oocyte-mediated nuclear reprogramming, many of which, we assume, are shared by other approaches, such as transcription factor-mediated reversal to pluripotency. We expect that a combination of approaches aimed at understanding the mechanisms of reprogramming will contribute to the development of safe and efficient methods for generating autologous cells for transplantation.

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Authors and Affiliations

  1. Division of Animal Sciences, School of Biosciences, Sutton Bonington Campus, University of Nottingham, Nottingham, LE12 5RD, UK

    Ramiro Alberio

  2. Centre for Genetics and Genomics, University of Nottingham, Nottingham, NG7 2UH, UK

    Ramiro Alberio

  3. Centre for Genetics and Genomics, University of Nottingham, Nottingham, UK

    Andrew D. Johnson

  4. School of Biology, Queen’s Medical Centre, University of Nottingham, Nottingham, UK

    Andrew D. Johnson

Authors
  1. Ramiro Alberio
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  2. Andrew D. Johnson
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Correspondence to Ramiro Alberio .

Editor information

Editors and Affiliations

  1. Leeds Institute of Genetics,, Health & Therapeutics, University of Leeds, Leeds, LS2 9JT, United Kingdom

    Justin Ainscough

  2. Inst. Frontier Medical Sciences, Kyoto University, Kawahara-cho 53, Kyoto, 606-8507, Japan

    Shinya Yamanaka

  3. Inst. Frontier Medical Sciences, Kyoto University, Kawahara-cho 53, Kyoto, 606-8507, Japan

    Takashi Tada

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Alberio, R., Johnson, A.D. (2011). Epigenetic Reprogramming with Oocyte Molecules. In: Ainscough, J., Yamanaka, S., Tada, T. (eds) Nuclear Reprogramming and Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-225-0_5

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