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The Use and Development of TAL Effector Nucleases

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Genome Editing

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB))

Abstract

In 2009 plant geneticists described a novel DNA binding domain derived from transcription activator-like effectors (TALEs) of the plant pathogen genus Xanthomonas. The DNA recognition domain was distinguished by a modular structure in which each building block binds to a single DNA nucleotide. The breakthrough was the identification of the key residues within each block that define its DNA binding properties and to show that specific alterations of these residues allow for the assembly of tailored DNA binding domains able to target any given sequence. This discovery set the stage for the generation of various designer proteins by fusing tailored TALE-based DNA binding domains, with either endonucleases, transcriptional modulators or chromatin remodeling domains, with the final purpose to modify the genome, the transcriptome or the epigenome. In the last few years, the exploitation of designer enzymes has expanded impressively with applications spanning from basic research to systems biology and human gene therapy.

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Authors and Affiliations

  1. Research Department, Cellectis SA, Paris, France

    Alexandre Juillerat Ph.D. & Philippe Duchateau Ph.D.

  2. Institute for Cell and Gene Therapy, Medical Center - University of Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany

    Toni Cathomen Ph.D.

  3. Center for Chronic Immunodeficiency, Medical Center - University of Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany

    Claudio Mussolino Ph.D.

Authors
  1. Alexandre Juillerat Ph.D.
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  2. Philippe Duchateau Ph.D.
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  3. Toni Cathomen Ph.D.
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  4. Claudio Mussolino Ph.D.
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Correspondence to Claudio Mussolino Ph.D. .

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Editors and Affiliations

  1. Inst for Cell & Gene Therapy, Universitaetsklinikum Freiburg, Freiburg, Baden-Württemberg, Germany

    Toni Cathomen

  2. University of North Carolina, Chapel Hill, North Carolina, USA

    Matthew Hirsch

  3. Stanford Medical School, Palo Alto, California, USA

    Matthew Porteus

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© 2016 American Society of Gene and Cell Therapy

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Juillerat, A., Duchateau, P., Cathomen, T., Mussolino, C. (2016). The Use and Development of TAL Effector Nucleases. In: Cathomen, T., Hirsch, M., Porteus, M. (eds) Genome Editing. Advances in Experimental Medicine and Biology(). Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3509-3_3

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