Skip to main content

The Strauss-Albertini Test for Direct Enumeration of Drug-Resistant Peripheral Blood Lymphocytes

  • Chapter
The Use of Human Cells for the Evaluation of Risk from Physical and Chemical Agents
  • 62 Accesses

Abstract

Our objective in developing the Strauss-Albertini test was to provide a direct technique for assessing the in vivo incidence of mutant cells arising amongst circulating lymphocytes in animals and man. The test is an early attempt at direct estimation of genotoxic risk from environmental agents. My present intention is to review the development and primary applications of the test, update the method, and nurture interest in this seminal system. Except where indicated otherwise, I performed the experiments described here while a graduate student at the College of Medicine, University of Vermont (USA).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Albertini, R.J., 1979, Direct Mutagenicity testing with peripheral blood lymphocytes, in: ‘Banbury Report 2, Mammalian Cell Mutagenesis: The Maturation of Test Systems“, A.W. Hsie, J.P. O’Neill, and V.K. McElheny, eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

    Google Scholar 

  • Albertini, R.J., and De Mars, R., 1973, Detection and quantification of X-ray induced mutation in cultured diploid human fibroblasts, Mutation Res., 18: 199.

    Article  PubMed  CAS  Google Scholar 

  • Albertini, R.J., and De Mars, R., 1974, Mosaicism of peripheral blood lymphocyte populations in females heterozygous for the Lesch-Nyhan mutation, Biochem. Genet., 11: 397.

    Article  PubMed  CAS  Google Scholar 

  • Bender, R.A., and Young, R.C., 1978, Effects of cancer treatment on individual and generational genetics, Seminars in Oncology, 5: 46.

    Google Scholar 

  • Dancis, J., Berman, P.H., Jansen, V., and Balis, M.E., 1978, Absence of mosaicism in the lymphocyte in X-linked congenital hyperuricemia, Life Sci., 7: 587.

    Article  Google Scholar 

  • De Mars, R., 1971, Genetic studies of HGPRT deficiency and the Lesch-Nyhan syndrome with cultured human cells, Fed. Proc. 30: 944.

    Google Scholar 

  • De Mars, R., and Held, K., 1972, The spontaneous azaguanineresistant mutants of diploid human fibroblasts, Humangenetic, 16: 87.

    Article  Google Scholar 

  • McKeran, R.O., Howell, A., Andrews, R.M., Watts, R.W.E., and Arlett, C.F., 1974, Observations on the growth in vitro of myeloid progenitor cells and fibroblasts from hemizygotes and heterozygotes for “complete” and “partial” hypoxanthine-guanine phosphoribosyltransferase deficiency, and their relevance to the pathogenesis of brain damage in the Lesch-Nyhan syndrome, J. Neurol. Sci., 72: 183.

    Article  Google Scholar 

  • Strauss, G.H.S., in press, Direct mutagenicity testing: The development of a clonal assay to detect and quantitate mutant lymphocytes arising in vivo, in: “Banbury Report 13, Indicators of Genotoxic Exposures in Man and Animals,” V.K. McElheny, ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

    Google Scholar 

  • Strauss, G.A., and Albertini, R.J., 1977, 6 Thioguanine resistant lymphocytes in human peripheral blood, in: “Progress in Genetic Toxicology,” D. Scott, B.A. Bridges, and F.H. Sobels, eds., Elsevier/North-Holland, Amsterdam.

    Google Scholar 

  • Strauss, G.H., and Albertini, R.J., 1979a, Enumeration of 6-thioguanine resistant peripheral blood lymphocyte frequencies in man as a potential test for somatic cell mutation arising in vivo, Mutation Res., 41: 353.

    Google Scholar 

  • Strauss, G.H., and Albertini, R.J., 1979b, Longitudinal determination of 6-thioguanine resistant blood lymphocyte frequencies in individuals receiving 8-methoxypsoralen and long-wave ultraviolet light treatment (PUVA), Environ. Mutagen, 1: 152.

    Google Scholar 

  • Strauss, G.H., Albertini, R.J., and Allen, B.J., 1980, An enumerative assay of purine analogue resistant lymphocytes in women heterozygous for the Lesch-Nyhan mutation, Biochem. Genet. 18: 529.

    Article  PubMed  CAS  Google Scholar 

  • Strauss, G.H., Albertini, R.J., Krusinski, P.A., and Baughman, R.D., 1979, 6-Thioguanine resistant peripheral blood lymphocytes in humans following psoralen, longwave ultraviolet light ( PUVA) therapy, J. Invest. Dermatol., 73: 211.

    Article  PubMed  CAS  Google Scholar 

  • Subak-Sharpe, J.H., Burk, R.A., and Pitts, J.D., 1979, Metabolic co-operation between biochemically marked mammalian cells in tissue culture, J. Cell. Sci., 4: 353.

    Google Scholar 

  • Thilly, W.G., De Luca, J.G. Hoppe, I.V.H., and Penman, B.W., 1976, Mutation of human lymphoblasts by methylnitrosourea, Chem. Biol. Interact., 15: 33.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Springer Science+Business Media New York

About this chapter

Cite this chapter

Strauss, G.H.S. (1983). The Strauss-Albertini Test for Direct Enumeration of Drug-Resistant Peripheral Blood Lymphocytes. In: Castellani, A. (eds) The Use of Human Cells for the Evaluation of Risk from Physical and Chemical Agents. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1117-2_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-1117-2_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1119-6

  • Online ISBN: 978-1-4757-1117-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics