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Isolation and Characterization of Mutant Plant Cell Cultures

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Plant Cell Culture in Crop Improvement

Part of the book series: Basic Life Sciences ((BLSC,volume 22))

Abstract

Mutant plant cell cultures are useful for biochemical and physiological studies, for markers in various genetic manipulation experiments and for the direct improvement of plants if the desired trait can be selected for and plants then be regenerated which express the trait. Many of the lines selected so far cannot truly be called mutants since genetic analysis, at the whole plant level, has not been carried out.

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References

  1. P. Maliga, Resistance mutants and their use in genetic manipulation, in: “Frontiers of plant Tissue Culture,” T. Thorpe, ed., University of Calgary, Calgary (1978).

    Google Scholar 

  2. P. Maliga, The need and the search for genetic markers in plant cell cultures, in: “Plant Cell Cultures: Results and Perspectives,” F. Sala, B. Parisi, R. Cella and O. Ciferri, eds., Elsevier/North-Holland Biomedical Press, Amsterdam (1980).

    Google Scholar 

  3. J. M. Widholm, The selection of agriculturally desirable traits with cultured plant cells, in: “Propagation of Higher Plants Through Tissue Culture — A Bridge Between Research and Application,” K.W. Hughes, R. Henke and M. Constantin, eds., Technical Info. Center, U.S. Dept. of Energy, Washington, DC (1978).

    Google Scholar 

  4. W. Handro, Mutagenesis and in vitro selection, in: “Plant Tissue Culture Methods and Applications in Agriculture,” T.A. Thorpe, ed., Academic Press, New York (1981).

    Google Scholar 

  5. D.S. Ingram, The expression of R-gene resistance to Phytophthora infestans in tissue cultures of Solanum tuberosum, J. Gen. Micro. 49:99–108 (1967).

    Article  CAS  Google Scholar 

  6. J.P. Helgeson, J.D. Kemp, G.T. Haberlach and D.P. Maxwell, A tissue culture system for studying disease resistance: the black shank disease in tobacco callus cultures, Phytopathol. 62:1439–1443 (1972).

    Article  Google Scholar 

  7. J. Ruyack, M.R. Downing, J.S. Chang and E.D. Mitchell, Growth of callus and suspension culture cells from cotton varieties (Gossypium hirsutum L.) resistant and susceptible to Xanthomonas malvacearum (E.F.SM.) Dows., In Vitro 15:368–373 (1979).

    Article  Google Scholar 

  8. B.G. Gengenbach, C.E. Green and C.M. Donovan, Inheritance of selected pathotoxin resistance in maize plants regenerated form cell cultures, Proc. Natl. Acad. Sci. USA 74:5113–5117 (1977).

    Article  PubMed  CAS  Google Scholar 

  9. R.I.S. Brettell, E. Thomas and D.S. Ingram, Reversion of Texas male-sterile cytoplasm maize in culture to give fertile, T-toxin resistant plants, Theor. Appl. Genet. 58:55–58 (1980).

    Google Scholar 

  10. B.G. Gengebach and C.E. Green, Selection of T-cytoplasm maize callus cultures resistant to Helminthosporium maydis race T pathotoxin, Crop Sci. 15:645–649 (1975).

    Article  Google Scholar 

  11. B.G. Gengenbach, J.A. Connelly, D.R. Pring and M.F. Conde, Mitochondrial DNA variation in maize plants regenerated during tissue culture selection, Theor. Appl. Genet. 59:161–167 (1981).

    Article  CAS  Google Scholar 

  12. M. Behnke, Selection of potato callus for resistance to culture filtrates of Phytophthora infestans and regeneration of resistant plants, Theor. Appl. Genet. 55:69–71 (1979).

    Article  Google Scholar 

  13. M. Behnke, Selection of dihaploid potato callus for resistance to the culture filtrate of Fusarium oxysporum, Z. Pflanzenzuchtg. 85:254–258 (1980).

    Google Scholar 

  14. T.H. Oswald, A.E. Smith and D.V. Phillips, Phytotoxicity and detoxification of metribuzin in dark grown suspension cultures of soybean, Pesticide Biochem. Physiol. 8:73–83 (1977).

    Article  Google Scholar 

  15. E.B. Swanson and D.T. Tomes, In vitro responses of tolerant and susceptible lines of Lotus corniculatus L. to 2,4-D, Crop Sci. 20:792–795 (1980).

    Article  CAS  Google Scholar 

  16. R.S. Chaleff and M.F. Parsons, Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacum, Proc. Natl. Acad. Sci. USA 75:5104–5107 (1978).

    Article  PubMed  CAS  Google Scholar 

  17. R.S. Chaleff, Further characterization of picloram-tolerant mutants, Theor. Appl. Genet. 58:91–95 (1980).

    CAS  Google Scholar 

  18. D.K. Miller and K.W. Hughes, Selection of paraquat-resistant variants of tobacco from cell cultures, In Vitro 16:1085–1091 (1980).

    Article  Google Scholar 

  19. D.N. Radin and P.S. Carlson, Herbicide-tolerant tobacco mutants selected in situ and recovered via regeneration from cell culture, Genetical Res. 32:85–89 (1978).

    Article  Google Scholar 

  20. M. Tal, H. Heikin and K. Dehan, Salt tolerance in the wild relatives of the cultivated tomato: responses of callus tissue of Lycopersicon esculentum, L. peruvianum and Solanum pennelli to high salinity, Z. Pflanzenphysiol. 86:231–240 (1978).

    CAS  Google Scholar 

  21. T.J. Orton, Comparison of salt tolerance between Hordeum vulgare and H. jubatum in whole plants and callus cultures, Z. Pflanzenphysiol. 98:105–118 (1980).

    CAS  Google Scholar 

  22. M.K. Smith and J.A. McComb, Effect of NaCl on the growth of whole plants and their corresponding callus cultues, Aust. J. Plant Physiol. 8:267–275 (1981).

    Article  CAS  Google Scholar 

  23. M.W. Nabor, S.E. Gibbs, C.S. Bernstein and M.E. Meis, NaCl-tolerant tobacco plants from cultured cells, Z. Pflanzenphysiol. 97:13–17 (1980).

    Google Scholar 

  24. A.K. Tyagi, A. Rashid and S.C. Maheshwari, Sodium chloride resistant cell line from haploid Datura innoxia Mill. A resistance trait carried from cell to plantlet and vice versa in vitro, Protoplasma 105:327–332 (1981).

    Article  Google Scholar 

  25. J.R. Wong and I.M. Sussex, Isolation of abscisic acid-resistant variants from tobacco cell cultures. I. Physiological bases for selection, Planta 148:97–102 (1980).

    Article  CAS  Google Scholar 

  26. J.R. Wong and I.M. Sussex, Isolation of abscisic acid-resistant variants from tobacco cell cultures. II. Selection and characterization of variants, Planta 148:103–107 (1980).

    Article  CAS  Google Scholar 

  27. J.M. Widholm, Differential expression of amino acid biosynthetic control isoenzymes in plants and cultured cells, in: “Plant Cell Cultures: Results and Perspectives,” F. Sala, B. Parisi, R. Cella and O. Ciferri, eds., Elsevier/North-Holland Biomedical Press, Amsterdam (1980).

    Google Scholar 

  28. B. Reisch, S.H. Duke and E.T. Bingham, Selection and characterization of ethionine-resistant alfalfa (Medicago sativa L.) cell lines, Theor. Appl. Genet. 59:89–94 (1981).

    Article  Google Scholar 

  29. S.W.J. Bright, P.B. Norbury and B.J. Miflin, Isolation of a recessive barley mutant resistant to S(2-aminoethyl)L-cysteine, Theor. Appl. Genet. 55:1–4 (1979).

    Article  CAS  Google Scholar 

  30. S.W.J. Bright, L.C. Featherstone and G.J. Miflin, Lysine metabolism in a barley mutant resistant to S(2-aminoethyl)cysteine, Planta 146:629–633 (1979).

    Article  CAS  Google Scholar 

  31. K.A. Hibberd, T. Walter, C.E. Green and B.G. Gengenbach, Selection and characterization of a feedback-insensitive tissue culture of maize, Planta 148:183–187 (1980).

    Article  CAS  Google Scholar 

  32. J.M. Widholm, Selection and characterization of cultured carrot and tobacco cells resistant to lysine, methionine and proline analogs, Can. J. Bot. 54:1523–1529 (1976).

    Article  CAS  Google Scholar 

  33. J.S.H. Kueh and S.W.J. Bright, Proline accumulation in a barley mutant resistant to trans-4-hydroxy-L-proline, Planta 153:166–171 (1981).

    Article  CAS  Google Scholar 

  34. J.E. Palmer and J.M. Widholm, Characterization of carrot and tobacco cells resistant to P-fluorophenylalanine, Plant Physiol. 56:233–238 (1975).

    Article  PubMed  CAS  Google Scholar 

  35. J. Berlin, Para-fluorophenylalanine resistant cell lines of tobacco, Z. Pflanzenphysiol. 97:317–324 (1980).

    CAS  Google Scholar 

  36. D.A. Evans and O.L. Gamborg, Effects of para-fluorophenylalanine on ploidy levels of cell suspension cultures of Datura innoxia, Environ. Expt. Bot. 19:269–275 (1979).

    Article  CAS  Google Scholar 

  37. C.E. Flick, R.A. Jensen and D.A. Evans, Isolation, protoplast culture, and plant regeneration of PFP-resistant variants of Nicotiana tabacum Su/Su, Z. Pflanzenphysiol. 103:239–245 (1981).

    Google Scholar 

  38. J.F. Thompson, J.T. Madison, M.A. Waterman and A.E. Muenster, Effect of methionine on growth and protein composition of cultured soybean cotyledons, Phytochem. 20:941–945 (1981).

    Article  CAS  Google Scholar 

  39. L. Wu and J. Antonovics, Zinc and copper tolerance of Agrostis stolonifera L. in tissue culture, Amer. J. Bot. 65:268–271 (1978).

    Article  CAS  Google Scholar 

  40. C.P. Meredith, Selection and characterization of aluminum-resistant variants from tomato cell cultures, Plant Sci. Lett. 12:25–34 (1978).

    Article  CAS  Google Scholar 

  41. C.M. Colijn, A.J. Kool and J.J.J. Nijkamp, An effective chemical mutagenesis procedure for Petunia hybrida cell suspension cultures, Theor. Appl. Genet. 55:101–106 (1979).

    Article  CAS  Google Scholar 

  42. Y.M. Heimer, J.L. Wray and P. Filner, The effect of tungstate on nitrate assimilation in higher plant tissues, Plant Physiol. 44:1197–1199 (1969).

    Article  PubMed  CAS  Google Scholar 

  43. J.M. Widholm, J.P. Ranch and K. Wakasa, Tungstate as a selective agent for high nitrate reductase activity in plant cells, In Vitro 17:211 (1981).

    Google Scholar 

  44. M.B. Berlyn, Isolation and characterization of isonicotinic acid hydrazide-resistant mutants of Nicotiana tabacum, Theor. Appl. Genet. 58:19–26 (1980).

    Article  CAS  Google Scholar 

  45. I. Zelitch and M.B. Berlyn, Altered glycine decarboxylation inhibition in isonicotinic acid hydrazide-resistant mutant callus lines and in regenerated plants and seed progeny, Plant Physiol. 69:198–204 (1982).

    Article  PubMed  CAS  Google Scholar 

  46. A.L. Lawyer, M.B. Berlyn and I. Zelitch, Isolation and characterization of glycine hydroxamate-resistant cell lines of Nicotiana tabacum, Plant Physiol. 66:334–341 (1980).

    Article  PubMed  CAS  Google Scholar 

  47. C.R. Somerville and W.L. Ogren, A phosphoglycolate phosphatase deficient mutant of Arabidopsis, Nature 280:833–836 (1979).

    Article  CAS  Google Scholar 

  48. C.R. Somerville and W.L. Ogren, Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate amino-transferase activity, Proc. Natl. Acad. Sci. USA 77:2684–2687 (1980).

    Article  PubMed  CAS  Google Scholar 

  49. P.J. Dix and H.E. Street, Selection of plant cell lines with enhanced chilling resistance, Ann. Bot. 40:903–910 (1976).

    Google Scholar 

  50. K.M. Templeton-Somers, W.R. Sharp and R.M. Pfister, Selection of cold-resistant cell lines of carrot, Z. Pflanzenphysiol. 103:139–148 (1981).

    CAS  Google Scholar 

  51. P.J. Dix, Chilling tolerance is not transmitted sexually in plants regenerated from Nicotiana sylvestris cell lines, Z. Pflanzenphysiol. 84:223–226 (1977).

    Google Scholar 

  52. J.M. Mastrangelo and H.H. Smith, Selection and differentiation of aminopterin resistant cells of Datura innoxia, Plant Sci. Lett. 10:171–179 (1977).

    Article  CAS  Google Scholar 

  53. A. Maretzki and M. Thom, Characteristics of a galactose-adapted sugarcane cell line grown in suspension culture, Plant Physiol. 61:544–548 (1978).

    Article  PubMed  CAS  Google Scholar 

  54. R.S. Chaleff and M.F. Parsons, Isolation of a glycerol-utilizing mutant of Nicotiana tabacum, Genetics 89:723–728 (1978).

    PubMed  CAS  Google Scholar 

  55. M. Limberg, D. Cress and K.G. Lark, Variants of soybean cells which can grow in suspension with maltose as a carbon-energy source, Plant Physiol. 63:718–721 (1979).

    Article  PubMed  CAS  Google Scholar 

  56. B. Reisch and E.T. Bingham, Plants from ethionine-resistant alfalfa tissue cultures: variation in growth and morphological characteristics, Crop Sci. 21:783–788 (1981).

    Article  CAS  Google Scholar 

  57. J.F. Shepard, D. Bidney and E. Shahin, Potato protoplasts in crop improvement, Science 208:17–24 (1980).

    Article  PubMed  CAS  Google Scholar 

  58. P.S. Carlson, Induction and isolation of auxotrophic mutants in somatic cell cultures of Nicotiana tabacum, Science 168:487–489 (1970).

    Article  PubMed  CAS  Google Scholar 

  59. R.L. Malmberg, Temperature-sensitive variants of Nicotiana tabacum isolated from somatic cell culture, Genetics 92:215–221 (1979).

    PubMed  CAS  Google Scholar 

  60. R.L. Malmberg, Biochemical, cellular and developmental characterization of a temperature-sensitive mutant of Nicotiana tabacum and its second site revertant, Cell 22:603–609 (1980).

    Article  PubMed  CAS  Google Scholar 

  61. J.C. Polacco, Arsenate as a potential negative selection agent for deficiency variants in cultured plant cells, Planta 146:155–160 (1979).

    Article  CAS  Google Scholar 

  62. A.J. Muller and R. Grafe, Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase, Molec. Gen. Genet. 161:67–76 (1978).

    Article  Google Scholar 

  63. J. King and V. Khanna, A nitrate reductase-less variant isolated from suspension cultures of Datura innoxia (Mill.), Plant Physiol. 66:632–636 (1980).

    Article  PubMed  CAS  Google Scholar 

  64. T.M. Murphy and C.W. Imbrie, Induction and characterization of chlorate-resistant strains of Rosa damascena cultured cells, Plant Physiol. 67:910–916 (1981).

    Article  PubMed  CAS  Google Scholar 

  65. J.M. Widholm, Utilization of indole analogs by carrot and tobacco cell tryptophan synthase in vivo and in vitro, Plant Physiol. 67:1101–1104 (1981).

    Article  PubMed  CAS  Google Scholar 

  66. A.D. Savage, J. King and O.L. Gamborg, Recovery of a pantothenate auxotroph from a cell suspension culture of Datura innoxia Mill., Plant Sci. Lett. 16:367–376 (1979).

    Article  CAS  Google Scholar 

  67. J. King, R.B. Horsch and A.D. Savage, Partial characterization of two stable auxotrophic cell strains of Datura innoxia Mill., Planta 149:480–484 (1980).

    Article  CAS  Google Scholar 

  68. V. Sidorov, L. Menczel and P. Maliga, Isoleucine-requiring Nicotiana plant deficient in threonine deaminase, Nature 194:87–88 (1981).

    Article  Google Scholar 

  69. A. Strauss, F. Bucher and P.J. King, Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus. I. A NO3 non-utilizing clone, Planta 153:75–80 (1981).

    Article  CAS  Google Scholar 

  70. C. Gebhardt, V. Schnebli and P.J. King, Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus. II. Auxotrophic and temperature-sensitive clones, Planta 153:81–89 (1981).

    Article  CAS  Google Scholar 

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Widholm, J.M. (1983). Isolation and Characterization of Mutant Plant Cell Cultures. In: Sen, S.K., Giles, K.L. (eds) Plant Cell Culture in Crop Improvement. Basic Life Sciences, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4379-0_9

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  • DOI: https://doi.org/10.1007/978-1-4684-4379-0_9

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