Abstract
It has been amply demonstrated over the past several years that nervous tissue can maintain and develop a high degree of differentiation in culture. Essentially every region of the neuraxis has been successfully grown in some form of culture, and neural tissue from bird, mouse, rat, and man as well as invertebrate material has proven suitable for in vitro work (Murray, 1971; Nelson, 1974). In this chapter, we will be particularly concerned with electrophysiological responses of nerve (and muscle) cells to iontophoretically applied neurohormones but will also deal with some biochemical effects of neuropharmacological agents.
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References
Bunge, R. P., Rees, R., Wood, P., Burton, H., Ko, C.-P.: 1974. Anatomical and physiological observations on synapses formed on isolated autonomic neurons in tissue culture, Brain Res. 66: 401–412.
Cohen, M. W.: 1972. The development of neuromuscular connexions in the presence of D-tubocurarine, Brain Res. 41: 457–463.
Cohen, S. A., Fischbach, G.: 1973. Regulation of muscle acetylcholine sensitivity by muscle activity in cell culture, Science 181: 76–78.
Crain, S. M.: 1972. Tissue culture model of epileptiform activity, in: Experimental Models of Epilepsy—A Manual for the Laboratory Worker ( D. P. Purpura, J. K. Penry, D. Tower, C. W. Woodbury, R. Walter, eds.), pp. 291–316, Raven Press, New York.
Crain, S. M.: 1973. Tissue culture studies of central nervous system maturation, in: Early Development, Res. Publ. Assoc. Res. Nerv. Ment. Dis. 51: 113–131.
Crain, S. M., Bornstein, M. B.: 1974. Early onset in inhibitory transactions during synaptogenesis in fetal mouse brain cultures, Brain Res. 68: 351–357.
Crain, S. M., Peterson, E. R.: 1971. Development of paired explants of fetal spinal cord and adult skeletal muscle during chronic exposure to curare and hemicholinium, In Vitro 6: 373.
Crain, S. M., Pollack, I.: 1973. Restorative effects of cyclic AMP on complex bioelectric activities of cultured fetal rodent CNS tissues after acute Ca++ deprivation, J. Neurobiol. 4: 321–342.
Crain, S. M., Bornstein, M. B., Peterson, E. R.: 1968. Maturation of cultured embryonic CNS tissue during chronic exposure to agents which prevent bioelectric activity, Brain Res. 8: 363–372.
Fischbach, G. D.: 1972. Synapse formation between dissociated nerve and muscle cells in low density cell cultures, Develop. Biol. 28: 407–429.
Fischbach, G. D., Cohen, S. A.: 1973. The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture, Develop. Biol. 31: 147–162.
Fischbach, G. D., Dichter, M. A.: 1974. Electrophysiologic and morphologic properties of neurons in dissociated chick spinal cord cell cultures, Develop. Neurol. 37: 100–116.
Giller, E. L., Schrier, B. K., Shainberg, A., Fisk, H. R., Nelson, P. G.: 1973. Choline acetyltransferase activity is increased in combined cultures of spinal cord and muscle cells from the mouse, Science 182: 588–589.
Giller, E. L., Breakefield, X. O., Christian, C. N., Neale, E. A., Nelson, P. G.: 1974. Expression of neuronal characteristics in culture: Some pros and cons of primary cultures and continuous cell lines, in: Proceedings of the Golgi Centennial Symposium (M. Santini, ed.), Raven Press, New York, in press.
Gilman, A., Nirenberg, M.: 1971. Effect of catecholamines on adenosine 3’–5’-cyclic monophosphate concentration of clonal satellite cells of neurons, Proc. Natl. Acad. Sci. 68: 2165–2168.
Godfrey, E. W., Nelson, P. G., Schrier, B. K., Breuer, A. C., Ransom, B. R.: 1975. Brain Res. (in press).
Harris, A. J., Dennis, M. J.: 1970. Acetylcholine sensitivity and distribution on mouse neuroblastoma cells, Science 67: 1253–1255.
Harris, A. J., Kuffler, S. W., Dennis, M. J.: 1971. Differential chemosensitivity of synaptic and extrasynaptic areas on the neuronal surface membrane in parasympathetic neurons of the frog tested by micro-application of acetylcholine, Proc. Roy. Soc. Lond. Ser. B 177: 541–553.
Hartzell, H. C., Fambrough, D. M.: 1973. Acetylcholine receptor production and incorporation into membranes of developing muscle fibers, Develop. Biol. 30: 153–165.
Hosli, E., Hosli, L.: 1971. Acetylcholinesterase in cultured rat spinal cord, Brain Res. 30: 193–197.
Hosli, E., Meier-Ruge, W., Hosli, L.: 1971. Monoamine-containing neurones in cultures of rat brain stem, Experientia 27: 310.
Hosli, E., Ljungdahl, A., Hokfelt, T., Hosli, L.: 1972. Spinal cord tissue cultures—A model for autoradiographic studies on uptake of putative neurotransmitters such as glycine and GABA, Experientia 28: 1342–1344.
Hosli, L., Hosli, E.: 1972. Autoradiographic localization of the uptake of glycine in cultures of rat medulla oblongata, Brain Res. 45: 612–616.
Hosli, L., Hosli, E., Res, P. G.: 1973a. Nervous tissue culture: A model to study action and uptake of putative neurotransmitters such as amino acids, Brain Res. 62: 597–602.
Hosli, L., Hosli, E., Res, P. F.: 19736. Electrophysiological and histochemical properties of fetal human spinal cord, in: Dynamics of Degeneration and Growth in Neurones, Wenner-Gren Center International Symposium, Stockholm.
Johnson, J. L.: 1972. Glutamic acid as a synaptic transmitter in the nervous system: A review, Brain Res. 37: 1–19.
Krnjevic, K.: 1974. Chemical nature of synaptic transmission in vertebrates, Physiol. Rev. 54: 418–540.
Krnjevic, K., Schwartz, S.: 1967. Some properties of unresponsive cells in the cerebral cortex, Exp. Brain Res. 3: 206–219.
Krnjevic, K., Pumain, R., Renaud, L.: 1971. The mechanism of excitation by acetylcholine in the cerebral cortex, J. Physiol. 215: 247–268.
Kuffler, S.T.W., Nicholls, J. G.: 1966. The physiology of neuroglial cells, Ergeb. Physiol. Biol. Chem. Exp. Pharmakol. 57: 1–90.
Lasher, R. S.: 1974. The uptake of [3H]GABA and differentiation of stellate neurons in cultures of dissociated postnatal rat cerebellum, Brain Res. 69: 235–254.
Murray, M. R.: 1971. Nervous tissues isolated in culture, in: Handbook of Neurochemistry, Vol. 5A ( A. Lajtha, ed.), pp. 373–438, Plenum, New York.
Nelson, P. G.: 1973. Electrophysiological studies of normal and neoplastic cells in tissue culture, in: Tissue Culture of the Nervous System ( G. Sato, ed.), pp. 135–160, Plenum, New York.
Nelson, P. G.: 1975. Nerve and muscle cells in culture, Physiol. Rev. 55: 1–6.
Nelson, P. G., Peacock, J. H.: 1973. Electrical activity in dissociated cell cultures from fetal mouse cerebellum, Brain Res. 61: 163–174.
Nelson, P., Ruffner, W., Nirenberg, M.: 1969. Neuronal tumor cells with excitable membranes grown in vitro, Proc. Natl. Acad. Sci. 64: 1004–1010.
Nelson, P. G., Peacock, J. H., Amano, T.: 1971. Responses of neuroblastoma cells to iontophoretically applied acetylcholine, J. Cell Physiol. 77: 353–362.
Olson, M. I., Bunge, R. P.: 1973. Anatomical observations on the specificity of synapse formation in tissue culture, Brain Res. 59: 19–33.
Paul, J.: 1972. Cell and Tissue Culture, Williams and Wilkins, Baltimore.
Peacock, J. H., Nelson, P. G.: 1973. Chemosensitivity of mouse neuroblastoma cells in vitro, J. Neurobiol. 4: 363–374.
Peacock, J. H., McMorris, F. A., Nelson, P. G.: 1973a. Electrical excitability and chemosensitivity of mouse neuroblastoma x mouse or human fibroblast hybrids, Exp. Cell Res. 79: 199–212.
Peacock, J. H., Nelson, P. G., Goldstone, M. W.: 1973b. Electrophysiologic study of cultured neurons dissociated from spinal cords and dorsal root ganglia of fetal mice, Develop. Biol. 30: 137–152.
Purves, D., Sakmann, B.: 1974. The effect of contractile activity on fibrillation and extrajunctional acetylcholine-sensitivity in rat muscle maintained in organ culture, J. Physiol. 237: 157–182.
Rall, W., Burke, R. E., Smith, T. G., Nelson, P. G., Frank, K.: 1967. Dendritic location of synapses and possible mechanisms for the monosynaptic EPSP in motoneurons, J. Neurophysiol. 30: 1169–1193.
Sato, G.: (ed.), 1973. Tissue Culture of the Nervous System, Vol. 1 of Current Topics in Neurobiology, Plenum, New York.
Schon, F., Kelly, J. S.: 1974. Autoradiographic localization of [3H]GABA and [H3]glutamate over satellite glial cells, Brain Res. 66: 275–288.
Schrier, B. K., Thompson, E. J.: 1974. On the role of glial cells in the mammalian nervous system, J. Biol. Chem. 219: 1769–1780.
Schubert, D., Harris, A. J., Heinemann, S., Kidokoro, Y., Patrick, J., Steinbach, J. H.: 1973. Differentiation and interaction of clonal cell lines of nerve and muscle, in: Tissue Culture of the Nervous System ( G. Sato, ed.), pp. 55–86, Plenum, New York.
Tasaki, I., Chang, J. J.: 1958. Electric response of glia cells in cat brain, Science 128: 1209–1210.
Wardell, W. M.: 1966. Electrical and pharmacological properties of mammalian neuroglial cells in tissue culture, Proc. Roy. Soc. Lond. Ser. B 165: 326–361.
Werman, R.: 1966. Criteria for identification of a central nervous system transmitter, Comp. Biochem. Physiol. 18: 745–766.
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Ransom, B.R., Nelson, P.G. (1975). Neuropharmacological Responses from Nerve Cells in Tissue Culture. In: Iversen, L.L., Iversen, S.D., Snyder, S.H. (eds) Principles of Receptor Research. Handbook of Psychopharmacology, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3168-1_4
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DOI: https://doi.org/10.1007/978-1-4684-3168-1_4
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