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Electrophysiological Studies of Normal and Neoplastic Cells in Tissue Culture

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Tissue Culture of the Nervous System

Part of the book series: Current Topics in Neurobiology ((CTNB))

Abstract

A number of tissue culture preparations have been extensively studied electrophysiologically, and this chapter will seek to summarize some of the observations which have been made on a number of continuous cell lines and on long-term cultures of normal, dissociated nerve and muscle cells. No attempt will be made to cover the valuable studies of nerve and muscle explants which have appeared over the past several years (Corner and Crain, 1972; Crain et al., 1970; Peterson and Crain, 1970).

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References

  • Augusti-Tocco, G., and Sato, G. 1969. Establishment of functional clonal lines of neurons from mouse neuroblastoma. Proc. Natl. Acad. Sci. 64:311–315.

    Article  PubMed  CAS  Google Scholar 

  • Azarnia, R., and Loewenstein, W. R. 1971. Intercellular communication and tissue growth. V. A. cancer strain that fails to make permeable membrane junctions with normal cells. J. Membrane Biol. 6:368–385.

    Article  Google Scholar 

  • Bennett, M. V. L. 1966. Physiology of electrotonic junctions. Ann. N. Y. Acad. Sci. 137:509.

    Article  PubMed  CAS  Google Scholar 

  • Bennett, M. V. L., and Trinkaus, J. P. 1970. Electrical coupling between embryonic cells by way of extracellular space and specialized junctions. J. Cell Biol. 44:592–610.

    Article  PubMed  CAS  Google Scholar 

  • Corner, M. A., and Crain, S. M. 1972. Patterns of spontaneous bioelectric activity during maturation in culture of fetal rodent medulla and spinal cord tissues. J. Neurobiol. 3:25–45.

    Article  PubMed  CAS  Google Scholar 

  • Crain, S. M., Alfei, L., and Peterson, E. R. 1970. Neuromuscular transmission in cultures of adult human and rodent skeletal muscle after innervation in vitro by fetal rodent spinal cord. J. Neurobiol. 1: 471–490.

    Article  PubMed  CAS  Google Scholar 

  • Davidson, R. L. 1971. Regulation of gene expression in somatic cell hybrids: A review. In Vitro 6:411–426.

    Article  PubMed  CAS  Google Scholar 

  • Fambrough, D., and Rash, J. E. 1971. Development of acetylcholine sensitivity during myogenesis. Develop. Biol. 26:55–68.

    Article  PubMed  CAS  Google Scholar 

  • Fischbach, G. D. 1970. Synaptic potentials recorded in cell cultures of nerve and muscle. Science 169:1331–1333.

    Article  PubMed  CAS  Google Scholar 

  • Fischbach, G. D. 1972. Synapse formation between dissociated nerve and muscle cells in low density cultures. Develop. Biol. 28:407–429.

    Article  PubMed  CAS  Google Scholar 

  • Fischbach, G., and Cohen, S. A. The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture. Develop. Biol. 31:147–162.

    Google Scholar 

  • Fischbach, G. D., and Dichter, M. The action potential of chick dorsal root ganglion neurons in cell culture. (In preparation).

    Google Scholar 

  • Fischbach, G. D., Nameroff, M., and Nelson, P. G. 1971. Electrical properties of chick skeletal muscle fibers developing in cell culture. J. Cell. Physiol. 78:289–300.

    Article  PubMed  CAS  Google Scholar 

  • Furshpan, E. J., and Potter, D. 1959. Transmission at the giant motor synapse of the crayfish. J. Physiol. 145:289–325.

    PubMed  CAS  Google Scholar 

  • Furshpan, E. J., and Potter, D. D. 1968. Low resistance junctions between cells in embryo and tissue culture, pp. 95–127. In A. A. Moscona and A. Monray (eds.). Current Topics in Developmental Biology, Vol. 3. Academic Press, New York.

    Google Scholar 

  • Gilula, N. B., Reeves, O. R., and Steinbach, A. 1972. Metabolic coupling, ionic coupling and cell contacts. Nature 235:262–265.

    Article  PubMed  CAS  Google Scholar 

  • Harris, A. J., and Dennis, M. J. 1970. Acetylcholine sensitivity and distribution on mouse neuroblastoma. Science 167:1253–1255.

    Article  PubMed  CAS  Google Scholar 

  • Harris, A. J., Heinemann, S., Schubert, D., and Tarakis, H. 1971. Trophic interaction between cloned culture lines of nerve and muscle. Nature 231:296–301.

    Article  PubMed  CAS  Google Scholar 

  • Hild, W., and Tasaki, I. 1962. Morphological and physiological properties of neurons and glial cells in tissue culture. J. Neurophysiol. 25:277–304.

    PubMed  CAS  Google Scholar 

  • Hirakow, R., and DeHaan, R. L. 1970. Synchronization and the formation of nexal junction between isolated chick embryonic heart myocytes beating in culture. J. Cell Biol. 47:88a.

    Google Scholar 

  • Klebe, R. J., and Ruddle, F. H. 1969. Neuroblastoma: Cell culture analysis of a differentiating stem cell system. J. Cell Biol. 43:69a.

    Google Scholar 

  • Kolodny, G. M. 1971. Evidence for transfer of macromolecular RNA between mammalian cells in culture. Exptl. Cell Res. 65:313–324.

    Article  PubMed  CAS  Google Scholar 

  • Kolodny, G. M. 1972. Cell to cell transfer of RNA into transformed cells. J. Cell Physiol. 79:147–150.

    Article  PubMed  CAS  Google Scholar 

  • Konigsberg, I. R. 1963. Clonal analysis of myogenesis. Science 140:1273–1274.

    Article  PubMed  CAS  Google Scholar 

  • Lamb, J. F., and MacKinnon, M. G. A. 1971. Effect of ouabain and metabolic inhibitors of the Na and K movements and nucleotide contents of L-cells. J. Physiol. (Lond.) 213:665–682.

    CAS  Google Scholar 

  • Lamb, J. F., and McCall, D. 1972. Effect of prolonged ouabain treatment on Na, K, C1 and Ca concentration and fluxes in cultured human cells. J. Physiol. 225:599–618.

    PubMed  CAS  Google Scholar 

  • Lasher, R. S., and Zagon, I. S. 1972. The effect of potassium on neuronal differentiation in cultures of dissociated newborn rat cerebellum. Brain Res. 41:482–488.

    Article  PubMed  CAS  Google Scholar 

  • Libet, B., and Tosaka, T. 1970. Dopamine as a synaptic transmitter and modulator in sympathetic ganglion: A different mode of synaptic action. Proc. Natl. Acad. Sci. 67:667–673.

    Article  PubMed  CAS  Google Scholar 

  • Loewenstein, W. R., and Kanno, Y. 1964. Studies on an epithelial (gland) cell junction. I. Modifications of surface membrane permeability. J. Cell Biol. 22:565–586.

    Article  PubMed  CAS  Google Scholar 

  • Loewenstein, W. R., and Kanno, Y. 1967. Intercellular communication and tissue growth. I. Cancerous growth. J. Cell Biol. 33:225–234.

    Article  PubMed  CAS  Google Scholar 

  • Minna, J., Nelson, P. G., Peacock, J., Glazer, D., and Nirenberg, M. 1971. Genes for neuronal properties expressed in neuroblastoma X L-cell hybrids. Proc. Natl. Acad. Sci. 68:234–239.

    Article  PubMed  CAS  Google Scholar 

  • Minna, J., Glazer, D., and Nirenberg, M. 1972. Genetic dissection of neural properties using somatic cell hybrids. Nature New Biol. 235:225–231.

    PubMed  CAS  Google Scholar 

  • Nelson, P. G., and Peacock, J. 1972. Acetylcholine responses in L-cells. Science 177:1005–1007.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, P. G., Ruffner, B. W., and Nirenberg, M. 1969. Neuronal tumor cells with excitable membranes grown in vitro. Proc. Natl. Acad. Sci. 64:1004–1010.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, P. G., Peacock, J., and Amano, T. 1971a. Responses of neuroblastoma cells to iontophoretically applied acetylcholine. J. Cell. Physiol. 77:353–362.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, P. G., Peacock, J., Amano, T., and Minna, J. 1971b. Electrogenesis in mouse neuroblastoma cells in vitro. J. Cell. Physiol. 77:337–352.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, P. G., Peacock, J., and Minna, J. 1972. An active electrical response in fibroblasts. J. Gen. Physiol. 60:58–71.

    Article  PubMed  CAS  Google Scholar 

  • Okun, L. M. 1972. Isolated dorsal root ganglion neurons in culture: Cytological maturation and extension of electrically active processes. J. Neurobiol. 3:111–152.

    Article  PubMed  CAS  Google Scholar 

  • Peacock, J., and Nelson, P. G. 1973a. Electrophysiologic study of cultured neurons dissociated from spinal cords and dorsal root ganglia of fetal mice. Develop. Biol. 30: 137–152.

    Article  PubMed  CAS  Google Scholar 

  • Peacock, J., and Nelson, P. G. 1973b. Chemosensitivity of neuroblastoma cells. J. Neurobiol. (in press).

    Google Scholar 

  • Peacock, J., and Nelson, P. G. 1973c. Electrical activity in dissociated cell cultures from fetal mouse cerebellum. Brain Res. (in press).

    Google Scholar 

  • Peacock, J., Minna, J., Nelson, P. G., and Nirenberg, M. 1972. Use of aminopterin in selecting electrically active neuroblastoma cells. Exptl. Cell. Res. 73:367–377.

    Article  CAS  Google Scholar 

  • Peacock, J., McMorris, F. A., and Nelson, P. G. 1973. Electrical excitability and chemosensitivity of mouse neuroblastoma × mouse or human fibroblast hybrids. Exptl. Cell Res. (submitted for publication).

    Google Scholar 

  • Peterson, E. R., and Crain, S. M. 1970. Innervation in cultures of fetal rodent skeletal muscle by organotypic explants of spinal cord from different animals. Z. Zellforsch. Mikroskop. Anat. 106:1–21.

    Article  CAS  Google Scholar 

  • Potter, E. E., Furshpan, E. J., and Lennox, E. S. 1966. Connections between cells of the developing squid as revealed by electrophysiological methods. Proc. Natl. Acad. Sci. 55:328–336.

    Article  PubMed  CAS  Google Scholar 

  • Robbins, N., and Yonezawa, T. 1971. Physiological studies during formation and development of rat neuromuscular junctions in tissue culture. J. Gen. Physiol. 58:467–481.

    Article  PubMed  CAS  Google Scholar 

  • Ruddle, F. H. 1972. Linkage analysis using somatic cell hybrids. Advan. Hum. Gent. 3: 173–235.

    CAS  Google Scholar 

  • Scott, B. S. 1971. Effect of potassium on neuron survival in cultures of dissociated human nervous tissue. Exptl. Neurol. 30:297–308.

    Article  CAS  Google Scholar 

  • Scott, B. S., Engelbert, V. E., and Fisher, K. C. 1969. Morphological and physiological characteristics of dissociated chick embryo spinal ganglion cells in cultures. Exptl. Neurol. 23:230–248.

    Article  CAS  Google Scholar 

  • Sheridan, J. D. 1971. Dye movement and low-resistance junctions between reaggregated embryonic cells. Develop. Biol. 26:627–636.

    Article  PubMed  CAS  Google Scholar 

  • Stockdale, F., and Holzer, H. 1961. DNA synthesis and myogenesis. Exptl. Cell Res. 24: 508–519.

    Article  PubMed  CAS  Google Scholar 

  • Subak-Sharp, J. H., Burk, R. R., and Pitts, J. D. 1969. Metabolic cooperation between biochemically marked mammalian cells in tissue culture. J. Cell Sci. 4:353–367.

    Google Scholar 

  • Trachtenberg, M. G., Kornblith, P. L., and Hauptli, J. 1972. Biophysical properties of cultured human glial cells. Brain Res. 38:279–298.

    Article  PubMed  CAS  Google Scholar 

  • Varon, S., and Raiborn, C. W., Jr. 1969. Dissociation, fractionation and culture of embryonic brain cells. Brain Res. 12:180–199.

    Article  PubMed  CAS  Google Scholar 

  • Walker, F. D., and Hild, W. J. 1969. Neuroglia electrically coupled to neurons. Science 165:602–603.

    Article  PubMed  CAS  Google Scholar 

  • Wardell, W. M. 1966. Electrical and pharmacological properties of mammalian neuroglial cells in tissue culture. Proc. Roy. Soc. Lond. Ser. B 165:326–361.

    Article  CAS  Google Scholar 

  • Yaffe, D. 1969. Cellular aspects of muscle differentiation in vitro. Curr. Topics Develop. Biol. 4:31–77.

    Google Scholar 

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© 1973 Plenum Press, New York

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Nelson, P.G. (1973). Electrophysiological Studies of Normal and Neoplastic Cells in Tissue Culture. In: Sato, G. (eds) Tissue Culture of the Nervous System. Current Topics in Neurobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2904-6_6

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  • DOI: https://doi.org/10.1007/978-1-4684-2904-6_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-2906-0

  • Online ISBN: 978-1-4684-2904-6

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