Abstract
The technique of intracellular injection of horseradish peroxidase (HRP) has been successfully used by a number of workers in order to label physiologically characterised cells in the cochlear nucleus (e.g. Rhode et al., 1983a, b; Rouiller and Ryugo, 1984, in the cat). We have extended it to the guinea pig cochlear nucleus (Parker et al., 1987). In addition to permitting a correlation between cell morphology and physiological response characteristics, this technique has conveniently resulted in transneuronal transport of HRP. Transneuronal transport of HRP, well described in other systems, is the process by which HRP in the injected cell gains access to other neurones, whether or not their synaptic contacts are involved. The process is facilitated by a number of factors; e.g. a large injection of HRP into the cell, adequate physiological condition of the animal (and, therefore, of the donor and recipient cells), a long post-injection survival time (Nassel, 1981), and increased activity of the neurones involved by appropriate stimulation (Harrison et al., 1984). This communication describes the physiological characterisation and HRP-labelling of a single giant (large multipdlar) cell, the soma of which was situated in the guinea pig anteroventral cochlear nucleus (AVCN) , and the transneuronal transport of HRP from this cell into a variety of different cell types in the cochlear nucleus. A preliminary communication of these data has been made (parker et al., 1986).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Adams, J., 1977, Technical considerations on the use of horseradish peroxidase as a neuronal marker, Neurosci., 2:141–145.
Bourke, T. R., 1976, Electrical responses of neural units in the anteroventral cochlear nucleus of the cat, Ph. D. thesis, M. I. T. Press.
Brown, A. G. and Fyffe, R. E. W., 1984, Intracellular staining of mammalian neurones. Academic Press.
Evans, E. F., 1979a, Neuroleptanaesthesia for the guinea pig, Arch. Otolaryngol., 105:185–186.
Evans, E. F., 1979b, in: “Auditory Investigations: the Scientific and Technological Basis”, Beagley, ed., Oxford University Press, 324–367.
Evans, E. F. and Nelson, P. G., 1973, Responses of single neurons in the cochlear nucleus of the cat as a function of their location and anaesthetic state, Exp. Brain. Res., 17:402–427.
Hackney, C. M. and Osen, K. K., 1985, The cochlear nucleus of the guinea pig: some light microscopic observations, Neurosci. Letters, Suppl 21, S15.
Hanker, J. S., Yates, R. E., Metz, C. B. and Rustioni, A., 1977, A new, specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase. Histochem. J., 9, 789–792.
Harrison, P. J., Hultborn, H., Jankowska, E., Katz, R., Storai, B. and Zytnicki, D., 1984, Labelling of interneurones by retrograde transsynaptic transport of horseradish peroxidase from motoneurones in rats and cats, Neurosci. Lett., 45:15–19.
Nassel, D. R., 1981, Transneuronal labelling with horseradish peroxidase in the visual system of the house fly, Brain Res., 206:431–438.
Osen, K. K., 1969, Cytoarchitecture of the cochlear nuclei in the cat, J. Comp. Neurol., 136:453–489.
Osen, K. K., 1987, Anatomy of the mammalian cochlear nuclei; a review. This volume.
Parker, D. J., Evans, E. F. and Hackney, C. M., 1986, Correlations between single neurone receptive fields and morphology in the guinea pig cochlear nucleus, Brit. J. Audiol., Proceedings of Meeting on Experimental Studies of Hearing and Deafness, Brighton 1986.
Parker, D. J., Evans, E. F. and Hackney, C. M., 1987, Receptive field properties and temporal discharge patterns related to neuronal morphology in the guinea pig cochlear nucleus, Brit. J. Audiol., Proceedings of Meeting on Experimental Studies of Hearing and Deafness, Oxford 1987.
Pfeiffer, R. R., 1966, Classification of response patterns of spike discharge for units in the cochlear nucleus: tone burst stimulation, Exp. Brain. Res., 1:220–235.
Rhode, W. S., Oertel, D., and Smith, P. H., 1983a, Physiological response properties of cells labelled intracellularly with horseradish peroxidase in cat ventral cochlear nucleus, J. Comp. Neurol., 213:448–463.
Rhode, W. S., Smith, P. H. and Oertel, D., 1983b, Physiological response properties of cells labelled intracellularly with horseradish peroxidase in cat dorsal cochlear nucleus, J. Comp. Neurol., 213: 426–447.
Rouiller, E. M. and Ryugo, D. K., 1984, Intracellular marking of physiologically characterised cells in the ventral cochlear nucleus of the cat, J. Comp. Neurol., 225:167–186.
Wenthold, R. J., 1987, Glycine immunoreactivity in the cochlear nucleus of the guinea pig: evidence for a glycinergic pathway connecting the two cochlear nuclei. A. R. O. abstracts, 212–213.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Plenum Press, New York
About this chapter
Cite this chapter
Parker, D.J., Evans, E.F., Hackney, C.M. (1988). Cellular Connections Revealed by Transneuronal Transport of HRP in the Guinea Pig Cochlear Nucleus. In: Syka, J., Masterton, R.B. (eds) Auditory Pathway. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1300-7_13
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1300-7_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1302-1
Online ISBN: 978-1-4684-1300-7
eBook Packages: Springer Book Archive