Abstract
Poly(ADP-ribose) metabolism in vivo is carried out mainly by poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase (1). Under normal conditions the level of poly(ADP-ribose) is stable in cells but if the cells are treated with a carcinogen (e.g. an alkylating agent) the level of poly(ADP-ribose) sharply increases in response to DNA damage (2). The short half-life measured in vivo after treatment of cells with alkylating agents (2) suggests that polymer degradation might occur during its accumulation thereby explaining the great discrepancy between the amount of NAD consumption and polymer accumulation. To better understand the relationship that is involved in poly(ADP-ribose) synthesis and degradation we have reconstituted in vitro a poly(ADP-ribose) turnover system.
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Abbreviations
- HPLC:
-
high pressure liquid chromatography
- PCA:
-
perchloric acid
- DTT:
-
dithiothreitol
- TCA:
-
trichloroacetic acid
- 3-AB:
-
3-aminobenzamide
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© 1989 Springer-Verlag New York Inc.
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Menard, L., Thibault, L., Gaudreau, A., Poirier, G.G. (1989). Reconstitution of an In Vitro Poly(ADP-Ribose) Turnover System. In: Jacobson, M.K., Jacobson, E.L. (eds) ADP-Ribose Transfer Reactions. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-8507-7_10
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DOI: https://doi.org/10.1007/978-1-4615-8507-7_10
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4615-8509-1
Online ISBN: 978-1-4615-8507-7
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