Summary
The application of metabolic engineering to plant secondary metabolite products is hindered by a lack of quantitative information on fluxes in the metabolic pathways. Analysis of fluxes in metabolic pathways in response to an environmental or genetic manipulation can help identify rate-limiting steps. Since total productivity is the goal for enhanced production of a valuable secondary metabolite, the product of the biomass productivity times the metabolite specific yield should be optimized. This implies a systems analysis of both primary and secondary metabolic pathways. This chapter will provide an overview of a strategy using HPLC photodiode array analysis, TLC, and MS to obtain temporal concentrations of secondary metabolites, and in situ measurements using31P and13C NMR spectroscopy to monitor primary metabolism and physiology. The model system is hairy root cultures of Catharanthus roseus and indole alkaloid biosynthetic pathways.
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Shanks, J.V., Rijhwani, S.K., Morgan, J., Vani, S., Bhadra, R., Ho, CH. (1999). Quantification of Metabolic Fluxes for Metabolic Engineering of Plant Products. In: Fu, TJ., Singh, G., Curtis, W.R. (eds) Plant Cell and Tissue Culture for the Production of Food Ingredients. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4753-2_5
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DOI: https://doi.org/10.1007/978-1-4615-4753-2_5
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