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Evaluation of Bioindicators of Contaminant Exposure and Effects in Coastal Ecosystems

  • Chapter
Ecological Indicators

Abstract

The issue of chemical contamination of the coastal environment is gaining increasing importance in the balance between the need to use chemicals and the desire to conserve and protect the ecosystem. In grappling with the complexity of contaminant interactions in the aquatic environment (Fig. 28.1), it becomes evident, although not universally accepted, that to properly assess the impact of chemical pollution on the health of ecosystems, a better understanding of processes that are vital to the normal functioning of various components (e.g. individuals, populations, or communities) of the system must be developed. Accordingly, we have focused our investigations on contaminant interactions at subcellular, cellular, and tissue levels of marine organisms to identify indices that assess alterations in critical physiological and biological processes (Fig. 28.2), with the aim that some of these measurements may serve as early warning signals of individual, population, or community effects. The logic underlying such an approach is that for the pollutantinduced perturbations to be evident in the ecosystem, the first level of interaction has to be at the individual level. Moreover, cause and effect relationships between chemical contamination and observed changes at the population or community level are more difficult to establish than they are at the individual level.

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References

  • Addison, R. F. and A. J. Edwards. (1988). Hepatic microsomal mono-oxygenase activity in flounder Platichthys flesus from polluted sites in Langesundfjord and from mesocosms experimentally dosed with diesel oil and copper. Mar. Ecol. Prog. Ser., 46, 51–54.

    Article  Google Scholar 

  • Bickel, M. H. and S. Muelebach. (1980). Pharmacokinetics and ecodisposition of poly-halogenated hydrocarbons: Aspects and concepts. Drug Metabol. Rev., 11, 149–190.

    Article  Google Scholar 

  • Black, D. E., D. K. Phelps, R. L. Lapan. (1988). The effect of inherited contamination of egg and larval winter flounder, Pseudopleuronectes americanus. Mar. Environ. Res., 25, 45–62.

    Article  Google Scholar 

  • Buhler, D. R. and D. E. Williams. (1989). Enzymes involved in metabolism of PAH by fishes and other aquatic animals: Oxidative enzymes (or Phase I enzymes). In Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment, ed. U. Varanasi. 151–184. Boca Raton, FL, CRC Press, Inc.

    Google Scholar 

  • Casillas, E., M. S. Myers, L. D. Rhodes and B. B. McCain. (1985). Serum chemistry of diseased English sole, Parophrys vetulus Girard, from polluted areas of Puget Sound, Washington, J. Fish Diseases, 8, 437–449.

    Article  Google Scholar 

  • Casillas, E., D. Misitano, L. L. Johnson, L. D. Rhodes, T. K. Collier, J. E. Stein, B. B. McCain and U. Varanasi. (1991). Inducibility of spawning and reproductive success of female English sole (Parophrys vetulus) from urban and nonurban areas of Puget Sound, Washington. Mar. Environ. Res., 31, 99–122.

    Article  Google Scholar 

  • Collier, T. K. and U. Varanasi. (1991). Hepatic activities of xenobiotic metabolizing enzymes and biliary levels of xenobiotics in English sole (Parophrys vetulus) exposed to environmental contaminants. Arch. Environ. Contam. Toxicol. 20, 462–473.

    Article  Google Scholar 

  • Collier, T. K., J. E. Stein, R. J. Wallace and U. Varanasi. (1986). Xenobiotic metabolizing enzymes in spawning English sole (Parophrys vetulus) exposed to organic solvent extracts of marine sediments from contaminated and reference areas. Comp. Biochem. Physiol., 84C, 291–298.

    Google Scholar 

  • Collier, T. K., B-T. Eberhart, J. E. Stein and U. Varanasi. (1989). Aryl hydrocarbon hydroxylase — a ‘new’ monitoring tool in the Status & Trends Program. In Proc. Oceans’ 89, 608–610, Washington, DC, I.E.E.E.

    Chapter  Google Scholar 

  • Collier, T. K., S. D. Connor, B. F. Anulacion, B.-T. Le Eberhart, A. Goksøyr and U. Varanasi. (1992a). Using cytochrome P450 to monitor the aquatic environment: Initial results from regional and national surveys. Mar. Environ. Res. (in press).

    Google Scholar 

  • Collier, T. K., J. E. Stein, H. R. Sanborn, T. Horn, M. S. Myers and U. Varanasi. (1992b). Field studies of reproductive success in English sole (Parophrys vetulus): Correlations with bioindicators of maternal contaminant exposure. Sci. Total Environ. (In press).

    Google Scholar 

  • Collier, T. K., S. V. Singh, Y. C. Awasthi and U. Varanasi. (1992c). Hepatic xenobiotic metabolizing enzymes in two species of benthic fish showing different prevalences of contaminant-associated liver neoplasms. Toxicol. Appl. Pharmacol., 113, 319–321.

    Article  Google Scholar 

  • Connell, D. W. (1987). Age to PCB concentration relationship with the striped bass (Morone saxatilis) in the Hudson River and Long Island Sound. Chemosphere, 16, 1469–1474.

    Article  Google Scholar 

  • Cross, J. N. (1986). Seasonal changes in DDT and PCB concentrations in white croaker are related to the reproductive cycle. Coastal Water Res. News, 1, 2–3.

    Google Scholar 

  • Di Giulio, R. T., P. C. Washburn, R. J. Wenning, G. W. Winston and C. S. Jewell. (1989). Biochemical responses in aquatic animals: A review of determinants of oxidative stress. Environ. Toxicol. Chem., 8, 1103–1123.

    Article  Google Scholar 

  • Dunn, B. P., J. J. Black and A. Maccubbin. (1987). 32P-postlabeling analysis of aromatic DNA adducts in fish from polluted areas. Cancer Res., 47, 6543–6548.

    Google Scholar 

  • Forlin, L. and C. Haux. (1990). Sex differences in hepatic cytochrome P-450 monooxygenase activities in rainbow trout during an annual reproductive cycle. J. Endocrin., 124, 207–213.

    Article  Google Scholar 

  • Frith, C. H. and J. M. Ward. (1980). A morphologic classification of proliferative and neoplastic hepatic lesions in mice. J. Environ. Pathol. Toxicol., 3, 329–351.

    Google Scholar 

  • Guiney, P. D., M. J. Melancon, Jr., J. J. Lech and R. E. Peterson. (1981). Effects of egg and sperm maturation and spawning on the distribution of a polychlorinated biphenyl in rainbow trout. Toxicol. Appl. Pharmacol., 9, 667–674.

    Google Scholar 

  • Gupta, R. C. and K. Randerath. (1988). Analysis of DNA adducts by 32P-labeling and thin layer chromatography. In DNA repair, vol. 3, ed. E. C. Friedberg and P. H. Hanawalt 399–418. New York, Marcel Dekker, Inc.

    Google Scholar 

  • Habig, C. and R. T. DiGiulio. (1988). Free radical-mediated effects in channel catfish exposed to contaminated sediments. Proceedings of the Society of Environmental Toxicology and Chemistry: Ninth Annual Meeting. Abstract: 59.

    Google Scholar 

  • Harshbarger, J. C. and J. B. Clark. (1990). Epizootiology of neoplasms in bony fish from North America. Sci. Total Environ., 94, 1–32.

    Article  Google Scholar 

  • Hawkins, W. E., W. W. Walker, R. M. Overstreet, J. S. Lytle and T. F. Lytle. (1990). Carcinogenic effects of some polycyclic aromatic hydrocarbons on the Japanese medaka and guppy in waterborne exposures. Sci. Total Environ., 94, 155–167.

    Article  Google Scholar 

  • Hendricks, J. D., T. R. Meyers and D. W. Shelton. (1984). Histological progression of hepatic neoplasia in rainbow trout (Salmo gairdneri). National Cancer Institute Monograph Series, 65, 321–336.

    Google Scholar 

  • James, M. O. and P. J. Little. (1981). Polyhalogenated biphenyls and phenobarbital: evaluation as inducers of drug metabolizing enzymes in the sheepshead, Archosargus probatocephalus. Chem. Biol. Interact., 36, 229–248.

    Article  Google Scholar 

  • Johnson, L. L., E. Casillas, T. K. Collier, B. B. McCain and U. Varanasi. (1988). Contaminant effects on ovarian maturation in English sole (Parophrys vetulus) from Puget Sound, Washington. Can. J. Fish Aquat. Sci., 45, 2133–2146.

    Article  Google Scholar 

  • Johnson, L. L., E. Casillas, D. Misitano, T. K. Collier, J. E. Stein, B. B. McCain and U. Varanasi. (1989). Bioindicators of reproductive impairment in female English sole (Parophrys vetulus) exposed to environmental contaminants. In Proc. Oceans’ 89, 391–396. Washington, DC, I.E.E.E.

    Chapter  Google Scholar 

  • Jones, G. and W. H. Butler. (1975). Morphology of spontaneous and induced neoplasia. In Mouse Hepatic Neoplasia, ed. W. H. Butler and P. M. Newberne, 21–60. Amsterdam, The Netherlands, Elsevier Scientific Publishing Company.

    Google Scholar 

  • Jones, G. and W. H. Butler. (1978). Light microscopy of rat hepatic neoplasia. In Rat Hepatic Neoplasia, ed. P. M. Newberne and W. H. Butler, 114–141. Cambridge, MA, The MIT Press.

    Google Scholar 

  • Ketterer, B., B. Coles and D. J. Meyer. (1983). The role of glutathione in detoxication. Environ. Health Perspect., 49, 59–69.

    Article  Google Scholar 

  • Krahn, M. M., M. S. Myers, D. G. Burrows and D. C. Malins. (1984). Determination of metabolites of xenobiotics in bile of fish from polluted waterways. Xenobiotica, 14, 633–646.

    Article  Google Scholar 

  • Krahn, M. M., L. D. Rhodes, M. S. Myers, L. K. Moore, W. D. MacLeod, Jr. and D. C. Malins. (1986). Associations between metabolites of aromatic compounds in bile and the occurrence of hepatic lesions in English sole (Parophrys vetulus) from Puget Sound, Washington. Arch. Environ. Contam. and Toxicol., 15, 61–67.

    Article  Google Scholar 

  • Krahn, M. M., D. G. Burrows, W. D. MacLeod and D. C. Malins. (1987). Determination of individual metabolites of aromatic compounds in hydrolyzed bile of English sole (Parophrys vetulus) from polluted sites in Puget Sound, Washington. Arch. Environ. Contam. Toxicol., 16, 511–522.

    Article  Google Scholar 

  • Krahn, M. M., C. A. Wigren, R. W. Pearce, L. K. Moore, R. G. Bogar, W. D. MacLeod, Jr., S-L. Chan and D. W. Brown. (1988). Standard analytical procedures of the NOAA National Analytical Facility. New HPLC cleanup and revised extraction procedures for organic contaminants. NOAA Tech. Memo. F/NWC-153. 52 pp.

    Google Scholar 

  • Krahn, M. M., D. G. Burrows, G. M. Ylitalo, D. W. Brown, C. A. Wigren, T. K. Collier, S.-L. Chan and U. Varanasi. (1991). Mass spectrometric analysis for aromatic compounds in bile of fish sampled after the Exxon Valdez oil spill. Environ. Sci. Technol., 26, 116–126.

    Article  Google Scholar 

  • Landahl, J. T., B. B. McCain, M. S. Myers, L. D. Rhodes and D. W. Brown. (1990). Consistent associations between hepatic lesions in English sole (Parophrys vetulus) and polycyclic aromatic hydrocarbons in bottom sediment. Environ. Health Perspect., 89, 195–203.

    Article  Google Scholar 

  • Lindström-Seppa, P. (1985). Seasonal variation of the xenobiotic metabolizing enzyme activities in the liver of male and female vendace (Coregonus albula L.). Aquat. Toxicol., 6, 323–331.

    Article  Google Scholar 

  • Lindström-Seppa, P. and A. Oikari. (1990). Biotransformation activities of feral fish in waters receiving bleached pulp mill effluents. Environ. Toxicol. Chem., 9, 1415–1424.

    Article  Google Scholar 

  • Malins, D. C., B. B. McCain, D. W. Brown, S.-L. Chan, M. S. Myers, J. T. Landahl, P. G. Prohaska, A. J. Friedman, L. D. Rhodes, D. G. Burrows, W. D. Gronlund and H. O. Hodgins. (1984). Chemical pollutants in sediments and diseases of bottom-dwelling fish in Puget Sound, Washington. Environ. Sci. Technol., 18, 705–713.

    Article  Google Scholar 

  • Malins, D. C., B. B. McCain, J. T. Landahl, M. S. Myers, M. M. Krahn, D. W. Brown, S.-L. Chan and W. T. Roubal. (1988). Neoplastic and other diseases in fish in relation to toxic chemicals: an overview. Aquat. Toxicol., 11, 43–67.

    Article  Google Scholar 

  • McCain, B. B., D. W. Brown, M. M. Krahn, M. S. Myers, R. C. Clark, Jr., S.-L. Chan and D. C. Malins. (1988). Marine pollution problems, North American West Coast. Aquat. Toxicol., 11, 143–162.

    Article  Google Scholar 

  • Meyers, T. R. and J. D. Hendricks. (1982). A summary of tissue lesions in aquatic animals induced by controlled exposures to environmental contaminants, chemotherapeutic agents, and potential carcinogens. Mar. Fish. Rev., 44, 1–17.

    Google Scholar 

  • Moore, M. J., R. Smolowitz and J. J. Stegeman. (1989). Cellular alterations preceding neoplasia in Pseudopleuronectes americanus from Boston Harbor. Mar. Environ. Res., 28, 425–429.

    Article  Google Scholar 

  • Myers, M. S., L. D. Rhodes and B. B. McCain. (1987). Pathologic anatomy and patterns of occurrence of hepatic neoplasms, putative preneoplastic lesions, and other idiopathic hepatic conditions in English sole (Parophrys vetulus) from Puget Sound, Washington. J. Nat. Cancer Inst., 78, 333–363.

    Google Scholar 

  • Myers, M. S., L. D. Rhodes, M. M. Krahn, B. B. McCain, J. T. Landahl, S.-L. Chan and U. Varanasi. (1988). Liver carcinogenesis in English sole from Puget Sound: the importance of neoplasia-associated hepatic lesions as indicators of contaminant exposure. In Proceed. of First Ann. Mtg., 633–646. Seattle, Puget Sound Research. Puget Sound Water Quality Authority.

    Google Scholar 

  • Myers, M. S., J. T. Landahl, M. M. Krahn, L. L. Johnson and B. B. McCain. (1990). Overview of studies on liver carcinogenesis in English sole from Puget Sound; evidence for a xenobiotic chemical etiology I: Pathology and epizootiology. Sci. Total Environ., 94, 33–50.

    Article  Google Scholar 

  • Myers, M. S., O. P. Olson, L. L. Johnson, C. S. Stehr, T. Horn and U. Varanasi. (1992). Hepatic lesions other than neoplasms in subadult flatfish from Puget Sound, WA; relationships with indices of contaminant exposure. Marine Environ. Res. (In press).

    Google Scholar 

  • O’Conner, J. M. and R. J. Huggett. (1988). Aquatic pollution problems, North Atlantic coast, including Chesapeake Bay. Aquat. Toxicol., 11, 163–190.

    Article  Google Scholar 

  • Pajor, A. M., J. J. Stegeman, P. Thomas and B. R. Woodin. (1990). Feminization of the hepatic microsomal cytochrome P-450 system in brook trout by estradiol, testosterone, and pituitary factors. J. Exp. Zool., 253, 51–60.

    Article  Google Scholar 

  • Payne, J. F., L. L. Fancey, A. D. Rahimutula and E. L. Porter. (1987). Review and perspective on the use of mixed-function oxygenase enzymes in biological monitoring. Comp. Biochem. Physiol., 86C, 233–245.

    Google Scholar 

  • Pitot, H. C. (1990). Altered hepatic foci: Their role in murine hepatocarcinogenesis. Annu. Rev. Pharmacol. Toxicol., 30, 465–500.

    Article  Google Scholar 

  • Plaa, G. L. (1986). Toxic responses of the liver. In Casarett and Doull’s toxicology: The basic science of poisons, 3rd edn, ed. C. D. Klaasen, M. O. Amdur and J. Doull, 286–309. New York. Macmillan.

    Google Scholar 

  • Plummer, J. L., B. R. Smith, L. M. Ball and J. R. Bend. (1980). Metabolism and biliary excretion of benzo(a)pyrene 4,5-oxide in the rat. Drug Metab. Dispos., 8, 68–72.

    Google Scholar 

  • Randerath, K., M. V. Reddy and R. C. Gupta. (1981). 32P-Labeling test for DNA damage. Proc. Natl. Acad. Sci. USA, 78, 6126.

    Article  Google Scholar 

  • Reichert, W. L. and U. Varanasi. (1982). Metabolism of orally administered naphthalene in adult English sole (Parophrys vetulus). Environ. Res., 27, 316–324.

    Article  Google Scholar 

  • Rhodes, L. D., M. S. Myers, W. D. Gronlund and B. B. McCain. (1987). Epizootic characteristics of hepatic and renal lesions in English sole, Parophrys vetulus, from Puget Sound. J. Fish. Biol., 31, 395–407.

    Article  Google Scholar 

  • Safe, S. (1984). Polychlorinated biphenyls (PCBs) and polybrominated biphenyls (PPBs): biochemistry, toxicology, and mechanism of action. Crit. Rev. Toxicol., 13, 319–395.

    Article  Google Scholar 

  • Schaaf, W. E., D. S. Peters, D. S. Vaughn, L. Coston-Clements and C. W. Krouse. (1987). Fish population responses to chronic and acute pollution: the influence of life history stages. Estuaries, 10, 267–275.

    Article  Google Scholar 

  • Schiewe, M. H., D. D. Weber, M. S. Myers, F. J. Jacques, W. L. Reichert, C. A. Krone, D. C. Malins, B. B. McCain, S.-L. Chan and U. Varanasi. (1991). Induction of foci of cellular alteration and other hepatic lesions in English sole (Parophrys vetulus) exposed to an extract of an urban marine sediment. Can. J. Fish. Aquat. Sci., 48, 1750–1760.

    Article  Google Scholar 

  • Shugart, L. R., S. M. Adams, B. D. Jimenez and S. S. Talmage. (1989). Biological markers to study exposure in animals and bioavailability of environmental contaminants. In ACS Symp. Ser. No. 382, Biological Monitoring for Pesticide Exposure: Measurement, Estimation, and Risk Reduction, ed. R. G. M. Wang, C. A. Franklin, R. C. Honeycutt and J. C. Reinert, American Chemical Society, Washington, DC, pp. 86–97.

    Google Scholar 

  • Sikka, H. C., J. P. Rutkowski, C. Kandaswami, S. Kumar, K. Earley and R. C. Gupta. (1990). Formation and persistence of DNA adducts in the liver of brown bullheads exposed to benzo[a]pyrene. Cancer Letters, 49, 81–87.

    Article  Google Scholar 

  • Sivarajah, K., C. S. Franklin and W. P. Williams. (1978). The effects of polychlorinated biphenyls on plasma steroid levels and hepatic microsomal enzymes in fish. J. Fish. Biol., 13, 401–409.

    Article  Google Scholar 

  • Spies, R. B. and D. W. Rice, Jr. (1988). Effects of organic contaminants on reproduction of the starry flounder Platichthys stellatus in San Francisco Bay. II. Reproductive success of fish captured in San Francisco Bay and spawned in the laboratory. Mar. Biol., 98, 191–200.

    Article  Google Scholar 

  • Stegeman, J. J. (1981). Polynuclear aromatic hydrocarbons and their metabolism in the marine environment. In Polycyclic hydrocarbons and cancer, vol. 3, ed. H. V. Gelboin and P.-O. P. Ts’o, 1–60. New York, Academic Press.

    Google Scholar 

  • Stehr, C. M. (1990). Ultrastructure of vacuolated cells in the liver of rock sole and winter flounder living in contaminated environments. Proceedings of the XIIth International Congress for Electron Microscopy, Abstract: 522–523.

    Google Scholar 

  • Stehr, C. M. and M. S. Myers. (1990). Ultrastructure and histology of cholangiocellular carcinomas in English sole (Parophrys vetulus) from Puget Sound, Washington. Toxicol. Pathol., 18, 28–55.

    Article  Google Scholar 

  • Stehr, C. M., L. D. Rhodes and M. S. Myers. (1988). The ultrastructure and histology of hepatocellular carcinomas of English sole (Parophrys vetulus) from Puget Sound, Washington. Toxicol. Pathol., 16, 418–431.

    Article  Google Scholar 

  • Stein, J. E., T. Horn and U. Varanasi. (1984). Simultaneous exposure of English sole (Parophrys vetulus) to sediment-associated xenobiotics: Part I. Uptake and disposition of [14C]polychlorinated biphenyls and [3H]benzo(a)pyrene. Mar. Environ. Res., 13, 97–119.

    Article  Google Scholar 

  • Stein, J. E., T. Horn, E. Casillas, A. Freidman and U. Varanasi. (1987). Simultaneous exposure of English sole (Parophrys vetulus) to sediment-associated xenobiotics: Part II. Chronic exposure to an urban sediment with added [3 H]benzo(a)pyrene and [14C]polych-lorinated biphenyls. Mar. Environ. Res., 22, 123–149.

    Article  Google Scholar 

  • Stein, J. E., W. L. Reichert, M. Nishimoto and U. Varanasi. (1989). 32P-postlabeling of DNA: A sensitive method for assessing environmentally induced genotoxicity. In Proc. Oceans’ 89, 385–390. Washington, DC, I.E.E.E.

    Chapter  Google Scholar 

  • Stein, J. E., T. Horn, H. R. Sanborn and U. Varanasi. (1991b). Effects of exposure to a contaminated-sediment extract on the metabolism and disposition of 17β-estradiol in English sole (Parophrys vetulus). Comp. Biochem. Physiol., 99C, 231–240.

    Google Scholar 

  • Stein, J. E., T. K. Collier, W. L. Reichert, E. Casillas, T. Horn and U. Varanasi. (1992). Bioindicators of contaminant exposure and sublethal effects: Studies with benthic fish in Puget Sound, WA. Environ. Toxicol. Chem., 11, 701–714.

    Article  Google Scholar 

  • Stewart, H. L., G. Williams, C. H. Keysser, L. S. Lombard and R. J. Montoli. (1980). Histologic typing of liver tumors in the rat. J. Natl. Cancer Inst., 64, 179–206.

    Google Scholar 

  • Swenberg, J. A., D. E. Rickert, B. L. Baranyi and J. I. Goodman. (1983). Cell specificity in DNA binding and repair of chemical carcinogens. Environ. Health Perspect., 49, 155–163.

    Article  Google Scholar 

  • Thomas, P. (1988). Reproductive endocrine function in female Atlantic croaker exposed to pollutants. Mar. Environ. Res., 24, 179–183.

    Article  Google Scholar 

  • Thomas, P. and H. W. Wofford. (1984). Effects of metals and organic compounds on hepatic glutathione, cysteine, and acid-soluble thiol levels in mullet (Mugil cephalus L). Toxicol. Appl. Pharmacol., 76, 172–182.

    Article  Google Scholar 

  • Varanasi, U. and D. J. Gmur. (1981). Hydrocarbons and metabolites in English sole (Parophrys vetulus) exposed simultaneously to [3H]benzo(a)pyrene and [14C]naphthalene in oil-contaminated sediment. Aquat. Toxicol., 1, 49–67.

    Article  Google Scholar 

  • Varanasi, U., M. Nishimoto, W. L. Reichert and J. E. Stein. (1982). Metabolism and subsequent covalent binding of benzo(a)pyrene to macromolecules in gonads and liver of ripe English sole (Parophrys vetulus). Xenobiotica, 12, 417–425.

    Article  Google Scholar 

  • Varanasi, U., T. K. Collier, D. E. Williams and D. R. Buhler. (1986). Hepatic cytochrome P-450 isozymes and aryl hydrocarbon hydroxylase in English sole (Parophrys vetulus). Biochem. Pharmacol., 35, 2967–2971.

    Article  Google Scholar 

  • Varanasi, U., J. E. Stein, M. Nishimoto, W. L. Reichert and T. K. Collier. (1987). Chemical carcinogenesis in feral fish: uptake, activation, and detoxication of organic xenobiotics. Environ. Health Perspect., 71, 155–170.

    Article  Google Scholar 

  • Varanasi, U., S.-L. Chan, B. B. McCain, J. T. Landahl, M. H. Schiewe, R. C. Clark, D. W. Brown, M. S. Myers, M. M. Krahn, W. D. Gronlund and W. D. MacLeod, Jr. (1989a). National Benthic Surveillance Project: Pacific Coast, Part II, Technical Presentation of the Results for Cycles I to III (1984–1986). NOAA Tech. Memo. NMFS F/NWC-170, 159 pp.

    Google Scholar 

  • Varanasi, U., J. E. Stein and M. Nishimoto. (1989b). Biotransformation and disposition of PAH in fish. In Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment, ed. U. Varanasi, 93–149. Boca Raton, FL, CRC Press.

    Google Scholar 

  • Varanasi, U., W. L. Reichert and J. E. Stein. (1989c). 32P-Postlabeling analysis of DNA adducts in liver of wild English sole (Parophrys vetulus) and winter flounder (Pseudopleuronectes americanus). Cancer Res., 49, 1171–1177.

    Google Scholar 

  • Varanasi, U., W. L. Reichert, B.-T. L. Eberhart and J. E. Stein. (1989d). Formation and persistence of benzo(a)pyrene-diolepoxide-DNA adducts in liver of English sole (Parophrys vetulus). Chem.-Biol. Interact., 69, 203–216.

    Article  Google Scholar 

  • Varanasi, U., S.-L. Chan, W. D. MacLeod, J. E. Stein, D. W. Brown, D. G. Burrows, K. L. Tilbury, J. T. Landahl, C. A. Wigren, T. Horn and S. M. Pierce. (1990). Survey of subsistence fish and shellfish for exposure to oil spilled from the EXXON VALDEZ, First Year: 1989, NOAA Tech. Memo. F/NWC-191, 151pp.

    Google Scholar 

  • Vogelbein, W. K., J. W. Fournie, P. A. Van Veld and R. J. Huggett. (1990). Hepatic neoplasms in the mummichog Fundulus heteroclitus from a creosote-contaminated site. Cancer Res., 50, 5978–5986.

    Google Scholar 

  • Walton, D. G., A. B. Acton and H. F. Stich. (1983a). DNA repair synthesis in cultured mammalian and fish cells following exposure to chemical mutagens. Mutation Res., 124, 153–161.

    Article  Google Scholar 

  • Walton, D. G., L. L. Fancey, J. M. Green, J. W. Kiceniuk and W. R. Penrose. (1983b). Seasonal changes in aryl hydrocarbon hydroxylase activity of a marine fish Tautogolabrus adspersus (Walbaum) with and without petroleum exposure. Comp. Biochem. Physiol. 76C, 247–253.

    Google Scholar 

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Varanasi, U., Stein, J.E., Johnson, L.L., Collier, T.K., Casillas, E., Myers, M.S. (1992). Evaluation of Bioindicators of Contaminant Exposure and Effects in Coastal Ecosystems. In: McKenzie, D.H., Hyatt, D.E., McDonald, V.J. (eds) Ecological Indicators. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4659-7_28

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