Abstract
Cell adhesion to neighboring cells and to the extracellular matrix (ECM) plays a major role in cell and tissue morphogenesis (Edelman, 1992; Takeichi, 1991; Hynes, 1992). These complex, adhesion-related cellular processes are mediated through transmembrane contact receptors of the cadherin and integrin families of receptors (Takeichi, 1991; Hynes, 1992). In the cytoplasmic domain, these receptors interact with cytoskeletal plaque proteins such as vinculin, talin and α-actinin which anchor the microfilament system to junctional areas in adherens type junctions (AJ) in adhesion plaques, and to α and β catenin and plakoglobin in cell-cell AJ (Burridge et al., 1988; Geiger and Ginsberg, 1991; Geiger et al., 1992). The cascade of molecular interactions which links the outside to the inside of the cell defines cell shape and motility, and also has a function in signal transduction which results in effects on cell growth, differentiation, and gene expression (Ben-Ze’ev, 1991; 1992; Schwartz, 1992; Haskill and Juliano, 1993). Signaling through adhesion plaques is suggested to occur through changes in tyrosine phosphorylation (Burridge et al., 1992; Volberg et al., 1992). Moreover, recent studies have demonstrated that the changes in tyrosine phosphorylation of a cytoplasmic adhesion plaque tyrosine kinase (p125FAK) is common to adhesion related signaling and to growth factor, cytokine and neuropeptide induced signaling (Zachary and Rozengurt, 1992), and that tyrosine phosphorylation of p125FAK is constitutively activated in oncogene-transformed cells (Guan and Shalloway, 1992). These results suggest a convergence, in adhesion plaques, of signals transduced by cytokines, oncogenes and adhesion.
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References
Belkin, A.M., O.I. Omatsky, A.E. Kabanov, M.A. Glukhova, and V.E. Koteliansky. (1988). Diversity of vinculin/meta-vinculin in human tissues and cultivated cells. J. Biol. Chem. 263: 14631–14635.
Bellas R. E., R. Bendori, and S. R. Farmer. (1991). Epidermal growth factor activation of vinculin and β-integrin gene transcription in quiescent Swiss 3T3 cells. J. Biol. Chem. 266: 12008–12014.
Bendori R., D. Salomon, and B. Geiger. (1987). Contact dependent regulation of vinculin expression in cultured fibroblasts: a study with vinculin specific cDNA probes. EMBO J. 6: 2897–2905.
Ben-Ze’ev, A. (1985). The cytoskeleton of cancer cells. Biochim. Biophys. Acta. 780: 197–212.
Ben-Ze’ev, A. (1991). Animal cell shape changes and gene expression. BioEssays 13: 207–212.
Ben-Ze’ev, A. (1992). Cytoarchitecture and signal transduction. Crit. Rev. Eukaryotic Gene Exp. 2: 265–281.
Ben-Ze’ev A., and A. Amsterdam. (1987). In vitro regulation of granulosa cell differentiation: involvement of cytoskeletal protein expression. J. Biol. Chem. 262: 5366–5376.
Ben-Ze’ev A., R. Reiss, R, Bendori, and B. Gorodecki. (1990). Transient induction of vinculin gene expression in 3T3 fibroblasts stimulated by serum growth factors. Cell Regul. 1: 621–636.
Burridge K., K. Fath, T. Kelly, G. Nuckolls, and C. Turner. (1988). Focal Adhesions: Transmembrane junctions between the extracellular matrix and the cytoskeleton. Ann. Rev. Cell. Biol. 4: 487–525.
Burridge K., C. E. Turner, and L. H. Romer. (1992). Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119: 893–903.
Edelman, G. M. (1992). Mediation and inhibition of cell adhesion by morphoregulatory molecules. Cold Spring Harb. Symp. Quant. Biol. LVII 317–325.
Geiger B., and D. Ginsberg. 1991. The cytoplasmic domain of adherens-type junctions. Cell Motil. Cytoskel. 20: 1–6.
Geiger B., O. Ayalon, D. Ginsberg, T. Volberg, J. L. Rodríguez Fernández, Y. Yarden, and A. Ben-Ze’ev. (1992). Cytoplasmic control of cell-adhesion. Cold Spring Harb. Symp. Quant, Biol. LVII 631–642.
Glück U., J. L. Rodríguez Fernández, R. Pankov, and A. Ben-Ze’ev. (1992). Regulation of adherens junction protein expression in growth-activated 3T3 cells and in regenerating liver. Exp. Cell. Res. 202: 477–486.
Glück U., D. J. Kwiatkowski, and A. Ben-Ze’ev. 1993. Suppression of tumorigenicity in simian virus 40-transformed 3T3 cells transfected with α-actinin cDNA. Proc. Natl. Acad. Sci. USA 90: 383–387.
Guan, J-L., and D. Shalloway. (1992). Regulation of focal adhesion associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation. Nature 358: 690–692.
Hynes, R.O. (1992). Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69: 11–25.
Juliano R. L., and S. Haskill. 1993. Signal transduction from the extracellular matrix. J. Cell Biol. 120: 577–585.
Kreis T. E., Z. Avnur, J. Schlessinger, and B. Geiger. (1984). Dynamic properties of cytoskeletal proteins in focal contacts, In Molecular Biology of the Cytoskeleton. G. Borisy, D. Cleveland, and D. Murphy, editors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 45–57.
Matsumura F., and Yamashiro-Matsumura, S. (1986). Tropomyosin in cell transformation. Cancer Rev. 6: 21–39.
Moiseyeva E. P., P. A. Weller, N. I. Zhidkova, E. B. Corben, B. Patel, I. Jasinska, V. E. Koteliansky, and D. R. Critchley. (1993). Organization of the human gene encoding the cytoskeletal protein vinculin and the sequence of the vinculin promoter. J. Biol. Chem. 268: 4318–4325.
Otey, C.A., F. M. Pavalko, and K. Burridge. (1990). An interaction between a-actinin and the b1 integrin subunit in vitro. J. Cell Biol. 111: 721–729.
Pollack, R., M. Osborn, and K. Weber. (1975). Patterns of organization of actin and myosin in normal and transformed cells. Proc. Natl. Acad. Sci. USA 72: 994–998.
Raz A., and A. Ben-Ze’ev. (1987). Cell contact and architecture of malignant cells and their relationship to metastasis. Cancer Met. Rev. 6: 3–21.
Raz A., M. Zöller, and A. Ben-Ze’ev. (1986). Cell configuration and adhesive properties of metastasizing and nonmetastasizing Bsp73 rat adenocarcinoma cells. Exp. Cell Res. 162: 127–141.
Rodríguez Fernández J. L., and A. Ben-Ze’ev. (1989). Regulation of fibronectin, integrin and cytoskeletal expression in differentiating adipocytes: inhibition by extracellular matrix and polylysine. Differentiation 42: 65–74.
Rodríguez Fernández J. L., B. Geiger, D, Salomon, I. Sabanay, M. Zöller, and A. Ben-Ze’ev. (1992a) Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA. J. Cell Biol. 119: 427–438.
Rodríguez Fernández J. L., B. Geiger, D. Salomon, and A. Ben-Ze’ev. (1992b). Overexpression of vinculin suppresses cell motility in Balb/C 3T3 cells. Cell Motil. Cytosk. 22: 127–134.
Rodríguez Fernández J. L., Geiger B., Salomon D., and Ben-Ze’ev, A. (1993). Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage dependent growth of 3T3 cells. J. Cell Biol. in press.
Schwartz, M. A. (1992). Transmembrane signaling by integrins. Trends Cell Biol. 2: 304–308.
Takeichi, M. (1991). Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251: 1451–1455.
Ungar F., B. Geiger, and A. Ben-Ze’ev. (1986). Cell contact and shape dependent regulation of vinculin synthesis in cultured fibroblasts. Nature 319: 787–791.
Vandekerckhove J., G. K. Bauw, G. Vancompernolle, B. Honore, and J. Celis (1990). Comparative two dimensional gel analysis and microsequencing identifies gelsolin as one of the most prominent downregulated markers of transformed human fibroblast and epthelial cells. J. Cell Biol. 11: 95–102.
Volberg T., Y. Zick, R. Dror, I, Sabanay, C. Gilon, A. Levitzki, and B. Geiger. (1992). The effect of tyrosine-specific protein phosphorylation on the assembly of adherens type junctions. EMBO J. 11: 1733–1742.
Zachary I., and E. Rozengurt. (1992). Focal adhesion kinase (p125FAK): a point of convergence in the action of neuropeptides, integrins, and oncogenes. Cell 71: 891–894.
Zieske J. D., G. Bukusoglu, and I. K. Gipson. (1989). Enhancement of vinculin synthesis by migrating stratified epithelium. J. Cell Biol. 109: 571–576.
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Ben-Ze’ev, A., Fernández, J.L.R., Glück, U., Salomon, D., Geiger, B. (1994). Changes in Adhesion Plaque Protein Levels Regulate Cell Motility And Tumorigenicity. In: Estes, J.E., Higgins, P.J. (eds) Actin. Advances in Experimental Medicine and Biology, vol 358. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2578-3_14
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DOI: https://doi.org/10.1007/978-1-4615-2578-3_14
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