Abstract
Infection of EBV negative B-lymphoid cells is a more relevant model to study the early events after fresh infection with EBV than infection of Raji cells already carrying EBV genomes and expressing latent gene products. We used BJA-B and BL02 cells for infection experiments with P3HR1 virus, which has no immortalizing capacity for umbilical cord blood lymphocytes, and B95–8, which is able to immortalize. We investigated the transcription and protein expression pattern and could demonstrate a lytic infection of BJA-B cells. The same viral genes known to be immediate early genes with transactivating properties from superinfection of Raji cells (1, 2) and which are also active in transient expression systems (3, 4, 5) seem to have a similar role in infected BJA-B cells.
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References
R. Seibl et al., in EBV and Human Dis., P.H. Levine, V.D. Ablashi et al.. Eds. (The Humana Press Inc., 1987), p. 211.
M. Biggin et al., J. Virol. 61, 3120 (1987)
A. Chevallier-Greco et al. EMBO 5/12, 3243 (1986)
M. Lieberman et al. J. Virol. 60, 140 (1986)
M. Hardwick et al. J. Virol. 62, 2274 (1988)
A. Pollack et al. Gene 27, 279 (1984)
M. Marschall et al. J. Virol. in press
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© 1989 The Humana Press Inc.
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Leser, U., Schwarzmann, F., Marschall, M., Wolf, H. (1989). Transcription and Protein Expression Pattern of EBV in Freshly Infected Lymphoid Cells. In: Ablashi, D.V., Faggioni, A., Krueger, G.R.F., Pagano, J.S., Pearson, G.R. (eds) Epstein-Barr Virus and Human Disease • 1988. Experimental Biology and Medicine, vol 20. Humana Press. https://doi.org/10.1007/978-1-4612-4508-7_8
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DOI: https://doi.org/10.1007/978-1-4612-4508-7_8
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