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Oxygen Transport in Bioreactors for Engineered Vascular Tissues

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Computational Modeling in Tissue Engineering

Part of the book series: Studies in Mechanobiology, Tissue Engineering and Biomaterials ((SMTEB,volume 10))

Abstract

Tissue engineered vascular grafts cultured in vitro are often done so under static conditions, which forces a diffusion-only mass transport regime for nutrient delivery and metabolite removal. Some bioreactor culture methods employ mechanical stimulation to improve material strength and stiffness; however, even with mechanical stimulation, engineered tissues are likely to operate in a diffusional transport regime for nutrient delivery and metabolite removal. In this study, we present an analysis of dissolved oxygen (DO) transport limitations that can arise in statically cultured vascular grafts and highlight bioreactor designs that improve transport, particularly by perfusion of medium through the interstitial space by transmural flow. A computational analysis is provided in conjunction with empirical data to support the models. Our goal was to investigate designs that would eliminate nutrient gradients that are evident using static culture methods in order to develop more uniform engineered vascular tissues, which could potentially improve mechanical strength and stiffness.

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Acknowledgments

This work has been supported by National Institutes of Health (NHLBI R01 HL083880 to RTT) and 3M Company (JWB). Furthermore, the technical assistance of Naomi Ferguson and Lee Meier is gratefully acknowledged as well as Dave Hultman for his efforts in machining and design discussions.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA

    Jason W. Bjork, Anton M. Safonov & Robert T. Tranquillo

  2. Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA

    Robert T. Tranquillo

Authors
  1. Jason W. Bjork
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  2. Anton M. Safonov
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  3. Robert T. Tranquillo
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Corresponding author

Correspondence to Robert T. Tranquillo .

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Editors and Affiliations

  1. , Biomechanics Research Unit, University of Liège, Chemin des Chevreuils 1–BAT 52/3, Liège 1, 4000, Belgium

    Liesbet Geris

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© 2012 Springer-Verlag Berlin Heidelberg

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Bjork, J.W., Safonov, A.M., Tranquillo, R.T. (2012). Oxygen Transport in Bioreactors for Engineered Vascular Tissues. In: Geris, L. (eds) Computational Modeling in Tissue Engineering. Studies in Mechanobiology, Tissue Engineering and Biomaterials, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8415_2012_133

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  • DOI: https://doi.org/10.1007/8415_2012_133

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-32562-5

  • Online ISBN: 978-3-642-32563-2

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