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Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift

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Fluorescent Methods to Study Biological Membranes

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 13))

Abstract

Biological membranes as an indispensable part of living organisms are permanently surrounded by the molecules of water. The presence of water is essential for maintaining their structure and functionality. Therefore, lipid bilayer hydration, mobility of the hydrated lipids, and their changes upon perturbations are appealing characteristics in the lipid membrane research. Time-dependent fluorescent shift (TDFS) measurements enable probing these properties in biologically relevant fully hydrated liquid crystalline lipid bilayers with a simple instrumentation and easy data treatment. Since the native lipid molecules do not fluoresce naturally, the extrinsic probing with a suitable fluorescent dye is necessary. There are a number of fluorescent membrane polarity probes designed for this purpose with different spectral properties and locations within the lipid bilayer. The basics of the technique are explained together with some useful additional considerations. The convenience of the TDFS method is demonstrated with examples from recent research on the study of the interactions of ions with lipid bilayers, and the monitoring of mobility and hydration changes along the bilayer normal upon addition of the oxidized phospholipids.

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Acknowledgments

The authors acknowledge support from the Czech Science Foundation via grants 208/10/1090 (SP and AO) and EUROMEMBRANES project MEM/09/E006 (MH, PJ). Moreover, MH acknowledges the Praemium Academie Award (Academy of Sciences of the Czech Republic).

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Authors and Affiliations

  1. J. Heyrovský Institute of Physical Chemistry of the ASCR, v. v. i., Dolejškova 3, 182 23, Prague 8, Czech Republic

    Sarka Pokorna, Agnieszka Olżyńska, Piotr Jurkiewicz & Martin Hof

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  1. Sarka Pokorna
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  2. Agnieszka Olżyńska
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  3. Piotr Jurkiewicz
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  4. Martin Hof
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Correspondence to Piotr Jurkiewicz .

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Editors and Affiliations

  1. Faculté de Pharmacie, Laboratoire de Biophotonique, Université de Strasbourg, route du Rhin 74, Illkirch, 67401, France

    Yves Mély

  2. Faculté de Pharmacie, Laboratoire de Biophotonique et, Université de Strasbourg, route du Rhin 74, Illkirch, 67401, France

    Guy Duportail

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Pokorna, S., Olżyńska, A., Jurkiewicz, P., Hof, M. (2012). Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift. In: Mély, Y., Duportail, G. (eds) Fluorescent Methods to Study Biological Membranes. Springer Series on Fluorescence, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2012_46

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