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Solvent Relaxation in Phospholipid Bilayers: Principles and Recent Applications

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Although there exist a number of methods, such as NMR, X-ray, e.g., which explore the hydration of phospholipid bilayers, the solvent relaxation (SR) method has the advantage of simple instrumentation, easy data treatment and possibility of measuring fully hydrated samples. The main information gained from SR by the analysis of recorded “time-resolved emission spectra” (TRES) is micro-viscosity and micro-polarity of the dye microenvironment. Based on these parameters, one can draw conclusions about water structure in the bilayer. In this review, we focus on physical background of this method, on all the procedures that are needed in order to obtain relevant parameters, and on the requirements on the fluorescence dyes. Furthermore, a few recent applications (the effect of curvature, binding of antibacterial peptides and phase transition) illustrating the versatility of this method are mentioned. Moreover, limitations and potential problems are discussed.

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ACKNOWLEDGMENTS

Financial support by the Czech Academy of Sciences (M.H., A.O., J.S, and P.J. via A400400503) and the Grant Agency of the Czech Republic (J.S. via 203/05/2308) is gratefully acknowledged.

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  1. J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejškova 3, CZ-18223, Prague, 8, Czech Republic

    Piotr Jurkiewicz, Jan Sýkora, Agnieszka Ol żyńska, Jana Humpolíčková & Martin Hof

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Jurkiewicz, P., Sýkora, J., Ol żyńska, A. et al. Solvent Relaxation in Phospholipid Bilayers: Principles and Recent Applications. J Fluoresc 15, 883–894 (2005). https://doi.org/10.1007/s10895-005-0013-4

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