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Human Pluripotent Stem Cells to Engineer Blood Vessels

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Engineering and Application of Pluripotent Stem Cells

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 163))

Abstract

Development of pluripotent stem cells (PSCs) is a remarkable scientific advancement that allows scientists to harness the power of regenerative medicine for potential treatment of disease using unaffected cells. PSCs provide a unique opportunity to study and combat cardiovascular diseases, which continue to claim the lives of thousands each day. Here, we discuss the differentiation of PSCs into vascular cells, investigation of the functional capabilities of the derived cells, and their utilization to engineer microvascular beds or vascular grafts for clinical application.

Graphical Abstract Human iPSCs generated from patients are differentiated toward ECs and perivascular cells for use in disease modeling, microvascular bed development, or vascular graft fabrication

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Notes

  1. 1.

    Note: Alterations to the root abbreviation are indicated in parentheses or brackets.

Abbreviations

2D:

Two-dimensional

3D:

Three-dimensional

bFGF:

Basic fibroblast growth factor

BMPR2:

Bone morphogenetic protein receptor type II

BP:

Burst pressure

CAD:

Coronary artery disease

CCD:

Chronic cardiovascular defects

DO:

Dissolved oxygen

DPI:

Diphenyleneiodonium

EB:

Embryoid body

EC:

Endothelial cell

ECM:

Extracellular matrix

EVC:

Early vascular cell

FBN1:

Fibrillin1

FPAH :

Family members of pulmonary arterial hypertension

HA:

Hyaluronic acid

(h)ESC:

(Human) embryonic stem cell

HIF:

Hypoxia-inducible factors

(h)[i]PSC:

(Human) [induced] pluripotent stem cell

HUVECs:

Human umbilical vein endothelial cells

ITA:

Internal thoracic artery

MFS:

Marfan syndrome

MMP:

Matrix metalloproteinase

PDGF-BB:

Platelet-derived growth factor-BB

PEG:

Poly(ethylene glycol)

PEGDA:

PEG-diacrylate

PGA:

Polyglycolic acid

ROS:

Reactive oxygen species

SMA:

Smooth muscle actin

SMMHC:

Smooth muscle myosin heavy chain

SRS:

Suture retention strength

(s)TEVG:

(Small-diameter) tissue engineered vascular graft

SV:

Saphenous vein

TESA:

Tissue engineering by self-assembly

TGFβ:

Transforming growth factor β

UMC:

Unaffected mutation carrier

VEGF(R):

Vascular endothelial growth factor (receptor)

vSMC:

Vascular smooth muscle cell

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Author notes

  1. Xin Yi Chan, Morgan B. Elliott and Bria Macklin contributed equally to this work.

Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, 21218, USA

    Xin Yi Chan, Morgan B. Elliott, Bria Macklin & Sharon Gerecht

Authors
  1. Xin Yi Chan
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  2. Morgan B. Elliott
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  3. Bria Macklin
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  4. Sharon Gerecht
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Corresponding author

Correspondence to Sharon Gerecht .

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Editors and Affiliations

  1. Leibniz Research Labs for Biotechnology, Hannover Medical School (MHH), Hannover, Germany

    Ulrich Martin

  2. Leibniz Research Labs for Biotechnology, Hannover Medical School (MHH), Hannover, Germany

    Robert Zweigerdt

  3. Leibniz Research Labs for Biotechnology, Hannover Medical School (MHH), Hannover, Germany

    Ina Gruh

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Chan, X.Y., Elliott, M.B., Macklin, B., Gerecht, S. (2017). Human Pluripotent Stem Cells to Engineer Blood Vessels. In: Martin, U., Zweigerdt, R., Gruh, I. (eds) Engineering and Application of Pluripotent Stem Cells. Advances in Biochemical Engineering/Biotechnology, vol 163. Springer, Cham. https://doi.org/10.1007/10_2017_28

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