Chapter

Telocytes

Volume 913 of the series Advances in Experimental Medicine and Biology pp 325-334

Date:

The Third Dimension of Telocytes Revealed by FIB-SEM Tomography

  • Dragos CretoiuAffiliated withVictor Babes National Institute of PathologyDivision of Cellular and Molecular Biology and Histology, Carol Davila University of Medicine and Pharmacy Email author 

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Abstract

Lately, spatial three-dimensional (3D) identity of cells and their interrelations with the environment that surrounds it represent a challenging trend with the purpose to achieve a holistic view over the functions. Combining data from different imaging of cells in the third dimension can offer insight into behavior modalities making a world of difference. This chapter outlines a breakthrough in telocyte research by volume electron microscopy with the aid of focused ion beam scanning electron microscopy (FIB-SEM). Reconstructing 3D (three-dimensional) appearance of telocytes from a set of two-dimensional (2D) images by FIB-SEM tomography allowed to extract valuable data about their volume in nanoscale dimensions such as the three-dimensional morphology of telopodes and extracellular vesicles.

Keywords

FIB SEM tomography Telocytes Telopodes Extracellular vesicles