Abstract
Lately, spatial three-dimensional (3D) identity of cells and their interrelations with the environment that surrounds it represent a challenging trend with the purpose to achieve a holistic view over the functions. Combining data from different imaging of cells in the third dimension can offer insight into behavior modalities making a world of difference. This chapter outlines a breakthrough in telocyte research by volume electron microscopy with the aid of focused ion beam scanning electron microscopy (FIB-SEM). Reconstructing 3D (three-dimensional) appearance of telocytes from a set of two-dimensional (2D) images by FIB-SEM tomography allowed to extract valuable data about their volume in nanoscale dimensions such as the three-dimensional morphology of telopodes and extracellular vesicles.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Popescu LM, Faussone-Pellegrini MS. TELOCYTES – a case of serendipity: the winding way from Interstitial Cells of Cajal (ICC), via Interstitial Cajal-Like Cells (ICLC) to TELOCYTES. J Cell Mol Med. 2010;14:729–40.
Cantarero I, Luesma MJ, Alvarez-Dotu JM, Munoz E, Junquera C. Transmission electron microscopy as key technique for the characterization of telocytes. Curr Stem Cell Res Ther. 2015;11:410–4.
Faussone Pellegrini MS, Popescu LM. Telocytes. Biomol Concepts. 2011;2:481–9.
Roatesi I, Radu BM, Cretoiu D, Cretoiu SM. Uterine telocytes: a review of current knowledge. Biol Reprod. 2015;93:10.
Gherghiceanu M, Popescu LM. Cardiac telocytes – their junctions and functional implications. Cell Tissue Res. 2012;348:265–79.
Cretoiu SM, Cretoiu D, Marin A, Radu BM, Popescu LM. Telocytes: ultrastructural, immunohistochemical and electrophysiological characteristics in human myometrium. Reproduction. 2013;145:357–70.
Fertig ET, Gherghiceanu M, Popescu LM. Extracellular vesicles release by cardiac telocytes: electron microscopy and electron tomography. J Cell Mol Med. 2014;18:1938–43.
Popescu LM, Fertig ET, Gherghiceanu M. Reaching out: junctions between cardiac telocytes and cardiac stem cells in culture. J Cell Mol Med. 2016;20:370–80.
Sun X, Zheng M, Zhang M, Qian M, Zheng Y, Li M, et al. Differences in the expression of chromosome 1 genes between lung telocytes and other cells: mesenchymal stem cells, fibroblasts, alveolar type II cells, airway epithelial cells and lymphocytes. J Cell Mol Med. 2014;18:801–10.
Zheng M, Sun X, Zhang M, Qian M, Zheng Y, Li M, et al. Variations of chromosomes 2 and 3 gene expression profiles among pulmonary telocytes, pneumocytes, airway cells, mesenchymal stem cells and lymphocytes. J Cell Mol Med. 2014;18:2044–60.
Song D, Cretoiu D, Zheng M, Qian M, Zhang M, Cretoiu SM, et al. Comparison of Chromosome 4 gene expression profile between lung telocytes and other local cell types. J Cell Mol Med. 2016;20:71–80.
Liu J, Cao Y, Song Y, Huang Q, Wang F, Yang W, et al. Telocytes in liver. Curr Stem Cell Res Ther. 2016;11:415–9.
Zhu Y, Zheng M, Song D, Ye L, Wang X. Global comparison of chromosome X genes of pulmonary telocytes with mesenchymal stem cells, fibroblasts, alveolar type II cells, airway epithelial cells, and lymphocytes. J Transl Med. 2015;13:318.
Cismasiu VB, Radu E, Popescu LM. miR-193 expression differentiates telocytes from other stromal cells. J Cell Mol Med. 2011;15:1071–4.
Zheng Y, Cretoiu D, Yan G, Cretoiu SM, Popescu LM, Wang X. Comparative proteomic analysis of human lung telocytes with fibroblasts. J Cell Mol Med. 2014;18:568–89.
Zheng Y, Cretoiu D, Yan G, Cretoiu SM, Popescu LM, Fang H, et al. Protein profiling of human lung telocytes and microvascular endothelial cells using iTRAQ quantitative proteomics. J Cell Mol Med. 2014;18:1035–59.
Albulescu R, Tanase C, Codrici E, Popescu DI, Cretoiu SM, Popescu LM. The secretome of myocardial telocytes modulates the activity of cardiac stem cells. J Cell Mol Med. 2015;19:1783–94.
Cretoiu SM, Radu BM, Banciu A, Banciu DD, Cretoiu D, Ceafalan LC, et al. Isolated human uterine telocytes: immunocytochemistry and electrophysiology of T-type calcium channels. Histochem Cell Biol. 2015;143:83–94.
Campeanu RA, Radu BM, Cretoiu SM, Banciu DD, Banciu A, Cretoiu D, et al. Near-infrared low-level laser stimulation of telocytes from human myometrium. Lasers Med Sci. 2014;29:1867–74.
Cretoiu SM, Popescu LM. Telocytes revisited. Biomol Concepts. 2014;5:353–69.
Bei Y, Wang F, Yang C, Xiao J. Telocytes in regenerative medicine. J Cell Mol Med. 2015;19:1441–54.
Vannucchi MG, Bani D, Faussone-Pellegrini MS. Telocytes contribute as cell progenitors and differentiation inductors in tissue regeneration. Curr Stem Cell Res Ther. 2016;11:383–9.
Popescu LM, Gherghiceanu M, Cretoiu D, Radu E. The connective connection: interstitial cells of Cajal (ICC) and ICC-like cells establish synapses with immunoreactive cells. Electron microscope study in situ. J Cell Mol Med. 2005;9:714–30.
Cretoiu D, Cretoiu SM, Simionescu AA, Popescu LM. Telocytes, a distinct type of cell among the stromal cells present in the lamina propria of jejunum. Histol Histopathol. 2012;27:1067–78.
Dawidowicz J, Szotek S, Matysiak N, Mielanczyk L, Maksymowicz K. Electron microscopy of human fascia lata: focus on telocytes. J Cell Mol Med. 2015;19:2500–6.
Cretoiu SM, Cretoiu D, Popescu LM. Human myometrium – the ultrastructural 3D network of telocytes. J Cell Mol Med. 2012;16:2844–9.
Peddie CJ, Collinson LM. Exploring the third dimension: volume electron microscopy comes of age. Micron. 2014;61:9–19.
Kizilyaprak C, Daraspe J, Humbel BM. Focused ion beam scanning electron microscopy in biology. J Microsc. 2014;254:109–14.
Narayan K, Danielson CM, Lagarec K, Lowekamp BC, Coffman P, Laquerre A, et al. Multi-resolution correlative focused ion beam scanning electron microscopy: applications to cell biology. J Struct Biol. 2014;185:278–84.
Cretoiu D, Hummel E, Zimmermann H, Gherghiceanu M, Popescu LM. Human cardiac telocytes: 3D imaging by FIB-SEM tomography. J Cell Mol Med. 2014;18:2157–64.
Cretoiu D, Gherghiceanu M, Hummel E, Zimmermann H, Simionescu O, Popescu LM. FIB-SEM tomography of human skin telocytes and their extracellular vesicles. J Cell Mol Med. 2015;19:714–22.
Acknowledgments
This project was supported by a grant of the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, project number 82/2012 and 194/2014.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media Singapore
About this chapter
Cite this chapter
Cretoiu, D. (2016). The Third Dimension of Telocytes Revealed by FIB-SEM Tomography. In: Wang, X., Cretoiu, D. (eds) Telocytes. Advances in Experimental Medicine and Biology, vol 913. Springer, Singapore. https://doi.org/10.1007/978-981-10-1061-3_21
Download citation
DOI: https://doi.org/10.1007/978-981-10-1061-3_21
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-1060-6
Online ISBN: 978-981-10-1061-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)