Skip to main content
SpringerLink
Log in

Moderately Halophilic, Exopolysaccharide-Producing Bacteria

  • Chapter
Halophilic Microorganisms

Abstract

Moderate halophiles include a wide array of microorganisms, taxonomically and physiologically distributed among many groups within the Bacteria domain and some groups of the Archaea. Their common characteristic is that they grow best at NaCl concentrations between 0.5 and 2.5 M (Kushner and Kamekura 1988), although they can be found in quite a diverse range of hypersaline habitats (Horikoshi and Grant 1998; Oren 1999).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Antón J, Meseguer I, Rodríguez-Valera F (1988) Production of an extracellular polysaccharide by Haloferax mediterranei. Appl Environ Microbiol 54: 2381–2386

    PubMed  Google Scholar 

  • Arias MS, Ferrer MR, Béjar V, del Moral A, Quesada E ( 2001 a) Viscoelastic, thixotropic and pseudoplastic properties of S-30, an extracellular polysaccharide produced by Halomonas maura. In: Ventosa A, Arahal DR (eds) International conference on halophilic microorganisms, Sevilla, Spain, abstract P54

    Google Scholar 

  • Arias MS, Páez R, Béjar V, del Moral A, Quesada E, Ferrer MR (2001b) Estudio comparativo del comportamiento reológico del exopolisacárido S-30 y otros aditivos empleados en la industria alimentaria. I National Congress of Science and Food Technology, Granada, Spain, abstract MV3

    Google Scholar 

  • Argandoña M, Béjar V, Quesada E, del Moral A (2001) Genome size and physical map of Halomonas maura, a new moderately halophilic microorganism. In: Ventosa A, Arahal DR (eds) International conference on halophilic microorganisms, Sevilla, Spain, abstract L-43

    Google Scholar 

  • Arvantis N, Vargas C, Tegos G, Perysinakis A, Nieto JJ, Ventosa A (1995) Development of gene reporter system in moderately halophilic bacteria by employing the ice nucleation gene of Pseudomonas syringae. Appl Environ Microbiol 61: 3821–3825

    Google Scholar 

  • Ashtaputre AA, Shah AK (1995) Studies on a viscous, gel-forming exopolysaccharide from Sphingomonas paucimobilis GS1.Appl Environ Microbiol 61: 1159–1162

    CAS  Google Scholar 

  • Béjar V, Calvo C, Moliz J, Diaz-Martínez F, Quesada E (1996) Effect of growth conditions on the rheological properties and chemical composition of Volcaniella eurihalina exopolysaccharide. Appl Biochem Biotechnol 59: 77–85

    Article  Google Scholar 

  • Béjar V, Llamas I, Calvo C, Quesada E (1998) Characterization of exopolysaccharides produced by 19 halophilic strains of the species Halomonas eurihalina. J Biotechnol 61: 135–141

    Article  Google Scholar 

  • Bouchotroch S, Quesada E, del Moral A, Béjar V (1999) Taxonomic study of exopolysaccharide-producing moderately halophilic bacteria isolated from hypersaline environments in Morocco. Syst Appl Microbiol 22: 412–419

    Article  CAS  Google Scholar 

  • Bouchotroch S, Quesada E, Izquierdo I, Rodríguez M, Béjar V (2000) Bacterial exopolysaccharides produced by new discovered bacteria belonging to the genus Halomonas isolated from hypersaline habitats in Morocco. J Ind Microbiol Biotechnol 24: 374–378

    Article  CAS  Google Scholar 

  • Bouchotroch S, Quesada E, del Moral A, Llamas I, Béjar V (2001) Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 51: 1625–1632

    Google Scholar 

  • Calvo C, Ferrer MR, Martínez-Checa F, Béjar V, Quesada E (1995) Some rheological properties of the extracellular polysaccharide produced by Volcaniella eurihalina F2–7. Appl Biochem Biotechnol 55: 45–54

    Article  CAS  Google Scholar 

  • Calvo C, Martínez-Checa F, Mota A, Béjar V, Quesada E (1998) Effect of cations, pH and sulfate content on the viscosity and emulsifying activity of the Halomonas eurihalina exopolysaccharide. J Ind Microbiol Biotechnol 20: 205–209

    Article  CAS  Google Scholar 

  • Cánovas D, Vargas C, Csonka LN, Ventosa A, Nieto JJ (1997) Isolation and characterization of salt-sensitive mutants of the moderate halophilic Halomonas elongata and cloning of the ectoine synthesis genes. J Biol Chem 272: 25794–25801

    Article  PubMed  Google Scholar 

  • Cánovas D, Vargas C, Kneip S, Morón MJ, Ventosa A, Bremer E, Nieto J (2000) Genes for the synthesis of the osmoprotectant glycine betaine from choline in the moderately halophilic bacterium Halomonas elongata. Microbiology 146: 455–463

    PubMed  Google Scholar 

  • Coronado MJ, Vargas C, Mellado E, Tegos G, Drainas C, Nieto J, Ventosa A (2000) The a-amylase gene amyH of the moderate halophile Halomonas meridiana: cloning and molecular characterization. Microbiology 146: 861–868

    PubMed  CAS  Google Scholar 

  • Dobson SJ, Franzmann PD (1996) Unification of the genera Deleya (Baumann et al 1983), Halomonas (Vreeland et al 1980) and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int J Syst Bacteriol 46: 77–83

    Article  Google Scholar 

  • Drainas C (200 1) Development of gene reporter systems in moderately halophilic bacteria. In: Ventosa A, Arahal DR (eds) International conference on halophilic microorganisms, Sevilla, Spain, abstract L-41

    Google Scholar 

  • Elkeles A, Rosenberg E, Ron EZ (1994) Production and secretion of polysaccharide biodispersan of Acinetobacter calcoaceticus A2 in protein secretion mutants. Appl Environ Microbiol 60: 4642–4645

    PubMed  CAS  Google Scholar 

  • Guezennec JC, Pignet P, Raquenes G, Deslandes E, Lijour Y, Gentric E (1994) Preliminary chemical characterization of unusual eubacterial exopolysaccharides of deep-sea origin. Carbohydr Polym 24: 287–294

    Article  CAS  Google Scholar 

  • Horikoshi K, Grant WD (eds) (1998) Extremophiles: microbial life in extreme environments. Wiley-Liss, New York

    Google Scholar 

  • Kushner DJ, Kamekura M (1988) Physiology of halophilic eubacteria. In: RodríguezValera F (ed) Halophilic bacteria, vol I. CRC Press, Boca Raton, pp 109–138

    Google Scholar 

  • Kunte HJ, Galinski EA (1995) Transposon mutagenesis in halophilic eubacteria: conjugal transfer and insertion of transposon Tn5 and Tn1732 in Halomonas elongata. FEMS Microbiol Lett 128: 293–299

    Article  PubMed  CAS  Google Scholar 

  • Llamas I, del Moral A, Béjar V, Girón MD, Salto R, Quesada E (1997) Plasmids from Halomonas eurihalina, a microorganism which produces an exopolysaccharide of biotechnological interest. FEMS Microbiol Lett 156: 251–257

    Article  CAS  Google Scholar 

  • Llamas I, Béjar V, Argandoña M, Quesada E, del Moral A (1999) Chemical mutagenesis of Halomonas eurihalina and selection of exopolysaccharide-deficient variants. Biotechnol Lett 21: 367–370

    Article  CAS  Google Scholar 

  • Llamas I, Argandoña A, Quesada E, del Moral A (2000) Transposon mutagenesis in Halomonas eurihalina. Res Microbiol 151: 13–18

    Article  PubMed  CAS  Google Scholar 

  • Llamas I, Sánchez MJ, Argandoña A, Béjar V, Quesada E, del Moral A (2002) Analysis of the genome of the moderate halophile Halomonas eurihalina. Curr Microbiol 45: 233–239

    Article  PubMed  CAS  Google Scholar 

  • Llamas I, Suárez A, Quesada E, del Moral A (2003) Characterization of the carAB operon, encoding the carbamoylphosphate synthase from Halomonas eurihalina. Extremophiles 7: 205–211

    PubMed  CAS  Google Scholar 

  • Louis P, Galinski EA (1997) Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in Escherichia coli. Microbiology 143: 1141–1149

    Article  PubMed  CAS  Google Scholar 

  • Martínez-Cánovas MJ, Liébana MC, Béjar V, Quesada E (1999) Bacterias halófilas productoras de exopolisacáridos. In: Abstracts of the XVII National Congress on Microbiology, Granada, Spain, abstract C10–49

    Google Scholar 

  • Martínez-Cánovas MJ, Mata J, Béjar V, Quesada E (2001) Estudio genotípico y filogenético de nuevos taxones de bacterias halófilas moderadas productoras de exopolisacáridos. In: Abstracts of the XVIII National Congress on Microbiology, Alicante, Spain, Abstract T15

    Google Scholar 

  • Martínez-Checa F, Calvo C, Caba MA, Ferrer MR, Béjar V, Quesada E (1996) Efecto de las condiciones nutricionales sobre la viscosidad y capacidad emulgente del biopolimero V2–7 de Volcaniella eurihalina. Microbiología SEM 12: 55–60

    Google Scholar 

  • Martínez-Checa F, Toledo FL, Quesada E, Calvo C (2002) Yield production, chemical composition and functional properties of emulsifier H28 synthesized by Halomonas eurihalina strain H-28 in media containing various hydrocarbons. Appl Microbiol Biotechnol 58: 358–363

    Article  PubMed  Google Scholar 

  • Mellado E, Asturias JA, Nieto JJ, Timmis KN, Ventosa A (1995a) Characterization of the basic replicon of pCM1, a narrow-host-range plasmid from the moderate halophile Chromohalobacter marismortui. J Bacteriol 177: 3443–3450

    PubMed  CAS  Google Scholar 

  • Mellado E, Moore ERB, Nieto JJ, Ventosa A (1995b) Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45: 712–716

    Article  PubMed  CAS  Google Scholar 

  • Mellado E, Nieto JJ, Ventosa A (1995 c) Construction of novel shuttle vectors for use between moderately halophilic bacteria and Escherichia coli. Plasmid 34: 157–164

    Google Scholar 

  • Mellado E, García MT, Nieto JJ, Kaplan S, Ventosa A (1997) Analysis of the genome of Vibrio costicola: pulsed-field gel electrophoretic analysis of genome size and plasmid contents. Syst Appl Microbiol 20: 20–26

    Article  Google Scholar 

  • Mellado E, García MT, Roldán E, Nieto J, Ventosa A (1998) Analysis of the genome of the gram-negative moderate halophiles Halomonas and Chromohalobacter by using pulsed-field gel electrophoresis. Extremophiles 2: 435–438

    Article  PubMed  CAS  Google Scholar 

  • Moraine RA, Rogovin P (1966) Kinetics of polysaccharide B-1459 fermentation. Biotechnol Bioeng 8: 511–524

    Article  CAS  Google Scholar 

  • Oren A (ed) (1999) Microbiology and biogeochemistry of hypersaline environments. CRC Press, Boca Raton

    Google Scholar 

  • Páez R, Morito JC, Llamas I, Quesada E (2000) Production of bacterial exopolysaccharides from sugar industry products. Abstracts of 14th Forum for Applied Biotechnology. Bruges, Belgium

    Google Scholar 

  • Pérez-Fernández ME, Quesada E, Gálvez J, Ruiz C (2000) Effect of exopolysaccharide V2–7 isolated from Halomonas eurihalina on the proliferation in vitro of human peripheral blood lymphocytes. Inmunopharmacol lmmunotoxicol 22: 131–141

    Article  Google Scholar 

  • Quesada E, Ventosa A, Ruiz-Berraquero F, Ramos-Cormenzana A (1984) Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34: 287–292

    Google Scholar 

  • Quesada E, Valderrama MJ, Béjar V, Ventosa A, Gutiérrez MC, Ruíz-Berraquero F, RamosCormenzana A (1990) Volcaniella eurihalina gen nov., sp. nov., a moderately halophilic nonmotile gram-negative rod. Int J Syst Bacteriol 40: 261–267

    Google Scholar 

  • Quesada E, Béjar V, Calvo C (1993) Exopolysaccharide production by Volcaniella eurihalina. Experientia 49: 1037–1041

    Article  CAS  Google Scholar 

  • Quesada E, del Moral A, Béjar V (1994) Comparative methods for isolation of Volcaniella eurihalina exopolysaccharide. Biotechnol Technol 8: 701–706

    Article  CAS  Google Scholar 

  • Quesada E, del Moral A, Ferrer MR, Béjar V (2001) Halomonas maura and Halomonas eurihalina: two bacteria which produce exopolysaccharides with emulsifying and viscosifying properties. In: Ventosa A, Arahal DR (eds) International conference on halophilic microorganisms, Sevilla, Spain, Abstract L-54

    Google Scholar 

  • Rodríguez-Valera F (1988) Halophilic bacteria, vol I. CRC Press, Boca Raton

    Google Scholar 

  • Rodríguez-Valera F (1991) General and applied aspects of halophilic microorganisms. Plenum Press, New York

    Book  Google Scholar 

  • Rodríguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A (1981) Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. Microb Ecol 7: 235–243

    Article  Google Scholar 

  • Swarbrick (1998) Dispersiones groseras. In: Gennaro AR (ed) Remington Farmacia, vol I, 19th edn. Editorial Medica Panamericana, Buenos Aires, pp 405–425

    Google Scholar 

  • Sutherland IW (1990) Biotechnology of microbial exopolysaccharides. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Sutherland IW (1998) Novel and established application of microbial polysaccharides. TIB Technol 16: 41–46

    Article  CAS  Google Scholar 

  • Sutherland IW (2001) Biofilm exopolysaccharides: a strong and sticky framework. Microbiology 147: 3–9

    PubMed  CAS  Google Scholar 

  • Tegos G, Vargas C, Perysinakis A, Koukkou AI, Christogianni A, Nieto JJ, Ventosa A, Drainas C (2000) Release of cell-free ice nuclei from Halomonas elongata expressing the ice nucleation gene inaZ of Pesudomonas syringae. J Appl Microbiol 89: 785–792

    Article  PubMed  CAS  Google Scholar 

  • Tombs M, Harding SE (1998) An introduction to polysaccharide biotechnology. Taylor and Francis, London

    Google Scholar 

  • Valderrama MJ, Quesada E, Béjar V, Ventosa A, Gutierrez MC, Ruíz-Berraquero F, RamosCormenzana A (1991) Deleya salina sp. nov. a moderately halophilic gram-negative bacterium. Int J Syst Bacteriol 41: 377–384

    Google Scholar 

  • Vargas C, Fernández-Castillo R, Cánovas D, Ventosa A (1995) Isolation of cryptic plasmids from moderately halophilic eubacteria of the genus Halomonas. Characterization of a small plasmid from Halomonas elongata and its use for shuttle vector construction. Mol Gen Genet 246: 411–418

    Article  PubMed  CAS  Google Scholar 

  • Ventosa A, Nieto JJ, Oren A (1998) Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62: 504–544

    PubMed  CAS  Google Scholar 

  • Vreeland RH (1992) The family Halomonadaceae. In: Ballows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, 2nd edn, vol I V. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Vreeland RH, Hochstein LI (1993) The biology of halophilic bacteria. CRC Press, Boca Raton

    Google Scholar 

  • Wilson PW Knight SC (eds) (1952) Experiments in bacterial physiology. Burguess, Minneapolis

    Google Scholar 

Download references

Authors
  1. Emilia Quesada
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Victoria Béjar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Rita Ferrer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Concepción Calvo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Inmaculada Llamas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fernando Martínez-Checa
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Soledad Arias
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Cristina Ruiz-García
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Rafael Páez
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. M. José Martínez-Cánovas
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Ana del Moral
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Microbiology and Parasitology Faculty of Pharmacy, University of Sevilla, 41012, Sevilla, Spain

    Antonio Ventosa

Rights and permissions

Reprints and permissions

Copyright information

© 2004 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Quesada, E. et al. (2004). Moderately Halophilic, Exopolysaccharide-Producing Bacteria. In: Ventosa, A. (eds) Halophilic Microorganisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-07656-9_22

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-07656-9_22

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05664-2

  • Online ISBN: 978-3-662-07656-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation