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The Third Dimension of Telocytes Revealed by FIB-SEM Tomography

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Telocytes

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 913))

Abstract

Lately, spatial three-dimensional (3D) identity of cells and their interrelations with the environment that surrounds it represent a challenging trend with the purpose to achieve a holistic view over the functions. Combining data from different imaging of cells in the third dimension can offer insight into behavior modalities making a world of difference. This chapter outlines a breakthrough in telocyte research by volume electron microscopy with the aid of focused ion beam scanning electron microscopy (FIB-SEM). Reconstructing 3D (three-dimensional) appearance of telocytes from a set of two-dimensional (2D) images by FIB-SEM tomography allowed to extract valuable data about their volume in nanoscale dimensions such as the three-dimensional morphology of telopodes and extracellular vesicles.

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Acknowledgments

This project was supported by a grant of the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, project number 82/2012 and 194/2014.

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Authors and Affiliations

  1. Victor Babes National Institute of Pathology, Bucharest, 050096, Romania

    Dragos Cretoiu

  2. Division of Cellular and Molecular Biology and Histology, Carol Davila University of Medicine and Pharmacy, Bucharest, 050474, Romania

    Dragos Cretoiu

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  1. Dragos Cretoiu
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Correspondence to Dragos Cretoiu .

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Editors and Affiliations

  1. Zhongshan Hospital, Fudan University Medical School, Shanghai, China

    Xiangdong Wang

  2. Department of Cellular and Molecular Med, Carol Davila Univ of Medicine&Pharmacy, Bucharest, Romania

    Dragos Cretoiu

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Cretoiu, D. (2016). The Third Dimension of Telocytes Revealed by FIB-SEM Tomography. In: Wang, X., Cretoiu, D. (eds) Telocytes. Advances in Experimental Medicine and Biology, vol 913. Springer, Singapore. https://doi.org/10.1007/978-981-10-1061-3_21

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