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Gene Therapy of Solid Cancers

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Wolfgang Walther

    1. Translational Oncology of Solid Tumors, Experimental and Clinical Research Center, Charité University Medicine Berlin and Max-Delbrück-Center for Molecular Medicine, Germany

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  2. Ulrike Stein

    1. Translational Oncology of Solid Tumors, Experimental and Clinical Research Center, Charité University Medicine Berlin and Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1317)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Immunogene Therapy

    1. Front Matter

      Pages 1-1
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    2. Aptamer Targeting the ERBB2 Receptor Tyrosine Kinase for Applications in Tumor Therapy

      • Georg Mahlknecht, Michael Sela, Yosef Yarden
      Pages 3-15

    3. Gene Gun Her2/neu DNA Vaccination: Evaluation of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model

      • Tam Nguyen-Hoai, Antonio Pezzutto, Jörg Westermann
      Pages 17-37

    4. MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine for Cancer Therapy

      • Manuel Schmidt, Barbara Volz, Patrick Großmann, Kerstin Heinrich, Burghardt Wittig
      Pages 39-51

  3. Suicide Gene Therapy

    1. Front Matter

      Pages 53-53
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    2. Evaluation of Bystander Cell Killing Effects in Suicide Gene Therapy of Cancer: Engineered Thymidylate Kinase (TMPK)/AZT Enzyme-Prodrug Axis

      • Takeya Sato, Anton Neschadim, Ryo Nakagawa, Teruyuki Yanagisawa, Jeffrey A. Medin
      Pages 55-67

    3. Oncoleaking: Use of the Pore-Forming Clostridium perfringens Enterotoxin (CPE) for Suicide Gene Therapy

      • Jessica Pahle, Jutta Aumann, Dennis Kobelt, Wolfgang Walther
      Pages 69-85

    4. iCaspase 9 Suicide Gene System

      • Xiaoou Zhou, Antonio Di Stasi, Malcolm K. Brenner
      Pages 87-105

  4. Gene Replacement Therapies

    1. Front Matter

      Pages 107-107
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    2. p53-Encoding pDNA Purification by Affinity Chromatography for Cancer Therapy

      • Ângela Sousa, João A. Queiroz, Fani Sousa
      Pages 109-124

    3. A qRT-PCR Method for Determining the Biodistribution Profile of a miR-34a Mimic

      • Kevin Kelnar, Andreas G. Bader
      Pages 125-133

  5. Gene Suppression and Signaling Modulation Therapy

    1. Front Matter

      Pages 135-135
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    2. Design and Selection of Antisense Oligonucleotides Targeting Transforming Growth Factor Beta (TGF-β) Isoform mRNAs for the Treatment of Solid Tumors

      • Frank Jaschinski, Hanna Korhonen, Michel Janicot
      Pages 137-151

    3. RNA Interference for Antimetastatic Therapy

      • Mathias Dahlmann, Ulrike Stein
      Pages 153-165

    4. STAT3 Decoy ODN Therapy for Cancer

      • Xiaoxia Sun, Jian Zhang
      Pages 167-183

  6. Oncolytic Virotherapy and Bacterial Vectors for Cancer Treatment

    1. Front Matter

      Pages 185-185
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    2. Oncolytic Viral Therapy Using Reovirus

      • Chandini Thirukkumaran, Don G. Morris
      Pages 187-223

    3. Use of GLV-1h68 for Vaccinia Virotherapy and Monitoring

      • Alexa Frentzen, Ulrike Geissinger, Desislava Tsoneva, Jochen Stritzker
      Pages 225-237

    4. Back to the Future: Are Tumor-Targeting Bacteria the Next-Generation Cancer Therapy?

      • Robert M. Hoffman
      Pages 239-260
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Keywords

About this book

This volume provides insight into recent developments on experimental and clinical strategies for cancer gene therapy. Gene Therapy of Solid Cancers: Methods and Protocols guides readers through protocols on gene therapeutic strategies in combination with helpful technical notes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, Gene Therapy of Solid Cancers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Translational Oncology of Solid Tumors, Experimental and Clinical Research Center, Charité University Medicine Berlin and Max-Delbrück-Center for Molecular Medicine, Germany

    Wolfgang Walther

  • Translational Oncology of Solid Tumors, Experimental and Clinical Research Center, Charité University Medicine Berlin and Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ulrike Stein

Bibliographic Information

  • Book Title: Gene Therapy of Solid Cancers

  • Book Subtitle: Methods and Protocols

  • Editors: Wolfgang Walther, Ulrike Stein

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2727-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2726-5Published: 15 June 2015

  • Softcover ISBN: 978-1-4939-5592-3Published: 09 October 2016

  • eBook ISBN: 978-1-4939-2727-2Published: 13 June 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 387

  • Number of Illustrations: 30 b/w illustrations, 30 illustrations in colour

  • Topics: Cancer Research, Gene Therapy

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