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Detection of bovine leukemia virus by the polymerase chain reaction in dried blood spots using a membrane system of a new format

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Moscow University Chemistry Bulletin Aims and scope

Abstract

Detection of bovine leukemia virus in dried blood spots by the polymerase chain reaction (PCR) was performed using a new format of membrane system for sample collection. The analysis results of liquid and dried samples of bovine whole blood were entirely consistent with each other and with the data of a veterinary laboratory. It was found that any part of a membrane strip with size of 0.5 × 0.5 cm containing an applied sample can be used for detection of a viral DNA copy. Provirus DNA in dried blood spots was stable for 8 days at 37°C and for 24 hours at 60°C. The new system for the sampling of whole blood in the form of dried spots can be used for sampling in field conditions for subsequent laboratory analysis.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119991, Russia

    J. V. Samsonova & A. S. Chadina

  2. National University of Science and Technology “MISiS”, Moscow, Russia

    A. P. Osipov & S. E. Kondakov

  3. Biocom, Moscow, Russia

    T. E. Makarova & A. B. Komarov

Authors
  1. J. V. Samsonova
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  2. A. S. Chadina
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  3. A. P. Osipov
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  4. S. E. Kondakov
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  5. T. E. Makarova
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  6. A. B. Komarov
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Corresponding author

Correspondence to J. V. Samsonova.

Additional information

Original Russian Text © J.V. Samsonova, A.S. Chadina, A.P. Osipov, S.E. Kondakov, T.E. Makarova, A.B. Komarov, 2014, published in Vestnik Moskovskogo Universiteta. Khimiya, 2014, No. 6, pp. 368–372.

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Samsonova, J.V., Chadina, A.S., Osipov, A.P. et al. Detection of bovine leukemia virus by the polymerase chain reaction in dried blood spots using a membrane system of a new format. Moscow Univ. Chem. Bull. 69, 282–285 (2014). https://doi.org/10.3103/S0027131414060066

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  • DOI: https://doi.org/10.3103/S0027131414060066

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